Organ structure and bacterial microbiogeography in a reproductive organ of the Hawaiian bobtail squid reveal dimensions of a defensive symbiosis
Applied and Environmental Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 15, 2025
ABSTRACT
Many
plants
and
animals
house
symbiotic
microorganisms
in
specialized
tissues
or
organs.
Here,
we
used
multidimensional
situ
imaging
techniques
to
illuminate
how
host
organ
structure
bacterial
microbiogeography
contribute
the
function
of
an
Hawaiian
bobtail
squid,
Euprymna
scolopes
.
Along
with
well-studied
light
organ,
female
E.
harbor
a
community
bacteria
accessory
nidamental
gland
(ANG).
The
ANG
is
dense
network
epithelium-lined
tubules,
some
which
are
dominated
by
single
taxon.
These
deposited
into
squid
eggs,
where
they
defend
developing
embryos
from
harmful
biofouling.
This
study
combination
visualize
different
dimensions
its
communities.
Imaging
entire
organs
sheet
microscopy
revealed
that
composite
tissue
individual,
non-intersecting
tubules
each
their
own
population.
bisected,
converging
toward
two
points
at
posterior
end.
At
these
points,
empty
space
can
mix
jelly
be
onto
eggs.
Observations
correlated
taxa
cell
morphology
tubule
populations
varied:
contained
mixed
taxa,
whereas
others
only
one
genus.
Together,
data
shed
on
interact
within
uses
physical
maintain
employ
population
defensive
context.
IMPORTANCE
Sequence-based
microbiome
studies
have
much
about
hosts
communities
microbiota
but
often
lack
spatial
understanding
microbes
relate
other
reside.
reveal
,
houses
diverse,
beneficial
deploys
them
for
egg
defense.
findings
suggest
partitioning
may
key
harboring
diverse
antimicrobial-producing
establishing
foundation
further
structures
mediate
interactions.
Язык: Английский
Artificial Selection and the Skin Microbiome Independently Predict Parasite Resistance
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 22, 2024
It
is
well
established
that
host
genetics
determine
much
of
hosts
response
to
parasites,
but
recent
research
has
highlighted
the
microbiome
plays
a
role
in
defense
against
parasites.
Interactions
between
resistance
parasites
and
are
widely
acknowledged;
for
example,
can
modulate
immune
response,
vice
versa.
However,
it
remains
unclear
how
system
may
together
influence
host's
overall
In
many
species
males
females
differ
both
their
genetically-based
parasite
composition
structure
associated
microbiomes.
We
therefore
expect
interactions
females,
potentially
explaining
often-observed
variation
To
investigate
interact
shape
we
imposed
truncation
selection
on
Trinidadian
guppies
(Poecilia
reticulata)
specialist
ectoparasite
Gyrodactylus
turnbulli.
The
30%
with
lowest
number
'parasite
load',
during
experimental
infection
founded
resistant
line,
highest
load
susceptible
while
randomly
chosen
uninfected
fish
control
line.
After
3-6
generations
breeding
absence
sampled
skin-associated
from
these
lines
then
infected
them
G.
used
Dirichlet
multinomial
modeling
(DMM)
machine
learning
identify
bacterial
community
types
across
evaluated
importance
line
type
over
time
'infection
severity'.
Among
developed
significantly
lower
severity,
higher
than
any
other
treatment.
males,
however,
severity
lines,
there
was
no
difference
lines.
also
found
female
had
tolerance
compared
or
females.
This
apparent
tradeoff
supported
by
analysis
individual-level
data.
host-associated
microbiomes
explain
as
genetics,
two
factors
appear
independent
additive
effects
severity.
Furthermore,
post-hoc
test
showed
model
including
artificial
better
at
models
either
variable
alone.
Our
results
suggest
an
active
interaction
not
passive
reflection
genetics.
Язык: Английский
Which microbiome are we talking about? Contrasted diversity patterns and eco-evolutionary processes between gill and intestinal microbiomes of Antarctic fairy shrimps
Frontiers in Ecology and Evolution,
Год журнала:
2024,
Номер
12
Опубликована: Ноя. 7, 2024
Metazoans
comprise
multiple
physical
niches
(“microenvironments”),
each
colonized
by
unique
microbiomes
that
contribute
to
their
hosts’
evolutionary
dynamics,
influencing
health,
physiology,
and
adaptation
changing
environments.
Most
wildlife
microbiome
studies
focus
on
higher
metazoans
host
microenvironments,
while
of
lower
species
often
concentrate
a
single
microenvironment,
sometimes
pooling
whole
bodies
or
specimens.
This
is
particularly
evident
in
small-sized
animals,
such
as
freshwater
meiofaunal
invertebrates,
thus
impeding
holistic
understanding
assembly
across
microenvironments
its
relation
with
population
genetics.
Leveraging
the
anostracan
fairy
shrimp
Branchinecta
,
which
has
easily
discernible
organs
expected
high
levels
intraspecific
genetic
divergence,
we
aimed
investigate
processes
test
phylosymbiosis
signal
two
(gill
intestine)
four
populations
gaini
within
Maritime
Antarctica,
using
16S
rRNA
metabarcoding.
Our
results
showed
gill
intestine
harbor
strikingly
different
resulting
from
B.
ecological
filtering
surrounding
environment
microbial
community.
Both
exhibit
respective
core
microbiomes,
yet
gill’s
narrower
constitutes
smaller
proportion
overall
bacterial
community
compared
intestine.
Within
(
i.e.
sampling
site),
assembles
through
distinct
eco-evolutionary
both
mostly
stochastically
(ecological
drift)
deterministically
(variable
selection)
Across
populations,
variable
selection
dominates
driving
compositional
divergence
microenvironment
although
lesser
extent
gill.
Lastly,
our
study
reveals
robust
correlation
between
structure
composition,
providing
evidence
anostracans.
Contrastingly,
was
less
pronounced
microbiome.
We
discuss
potential
differences
may
underlie
difference
strength
phylosymbiosis.
highlights
relevance
considering
testing
hypothesis
better
understand
intricate
relationships
hosts
microbiomes.
Язык: Английский
Experimental Evolution of a Mammalian Holobiont? Genetic and Maternal Effects on the Cecal Microbiome in Bank Voles Selectively Bred for Herbivorous Capability
Deleted Journal,
Год журнала:
2024,
Номер
97(5), С. 274 - 291
Опубликована: Авг. 15, 2024
AbstractMammalian
herbivory
represents
a
complex
adaptation
requiring
evolutionary
changes
across
all
levels
of
biological
organization,
from
molecules
to
morphology
behavior.
Explaining
the
evolution
such
traits
major
challenge
in
biology,
as
it
is
simultaneously
muddled
and
enlightened
by
growing
awareness
crucial
role
symbiotic
associations
shaping
organismal
adaptations.
The
concept
hologenomic
includes
partnered
unit
holobiont,
host
with
its
microbiome,
selection
that
may
undergo
adaptation.
Here,
we
test
some
assumptions
underlying
using
unique
experimental
model:
lines
bank
vole
(
Язык: Английский
A strong priority effect in the assembly of a specialized insect-microbe symbiosis
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 18, 2024
ABSTRACT
Specialized
host-microbe
symbioses
are
ecological
communities,
whose
composition
is
shaped
by
various
processes.
Microbial
community
assembly
in
these
determined
part
interactions
between
taxa
that
colonize
niches
available
within
habitat
patches.
The
outcomes
of
interactions,
and
extension
the
trajectory
assembly,
can
display
priority
effects—dependency
on
order
which
first
occupy
niches.
underlying
mechanisms
phenomena
vary
from
system
to
often
not
well
resolved.
Here,
we
characterize
effects
colonization
squash
bug
(
Anasa
tristis
)
bacterial
symbionts
genus
Caballeronia
,
using
pairs
strains
known
strongly
compete
during
host
colonization,
as
isogenic
thus
functionally
identical.
By
introducing
symbiont
into
individual
bugs
a
sequential
manner,
show
within-host
populations
established
colonist
extremely
resistant
invasion,
regardless
strain
identity
competitive
interactions.
knocking
down
population
an
initial
with
antibiotics,
further
success
second
still
diminished
even
when
space
symbiotic
organ
ostensibly
accessible
for
colonization.
We
speculate
resident
exclude
subsequent
infections
manipulating
environment,
partially
but
exclusively
eliciting
tissue
remodeling
organ.
IMPORTANCE
Host-associated
microbial
communities
underpin
critical
ecosystem
processes
human
health,
their
ability
do
so
turn
shape
composition.
While
selection
deterministically
acts
competing
genotypes
species
manner
determines
differ
depending
sequence
community.
document
this
phenomenon,
effect,
experimental
North
American
insect
pest,
its
betaproteobacterial
Caballeronia.
Our
study
demonstrates
how
stark,
strain-level
variation
emerge
specialized
simply
through
differences
host.
Understanding
mechanistic
drivers
structure
host-associated
microbiomes
highlight
both
pitfalls
opportunities
engineering
constituent
societal
benefit.
Язык: Английский
Organ structure and bacterial microbiogeography in a reproductive organ of the Hawaiian bobtail squid reveal dimensions of a defensive symbiosis
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 7, 2024
ABSTRACT
Many
plants
and
animals
house
symbiotic
microorganisms
in
specialized
tissues
or
organs.
Here,
we
used
multidimensional
in-situ
imaging
techniques
to
illuminate
how
host
organ
structure
bacterial
microbiogeography
contribute
the
function
of
an
Hawaiian
bobtail
squid,
Euprymna
scolopes
.
Along
with
well-studied
light
organ,
female
E.
harbor
a
community
bacteria
accessory
nidamental
gland
(ANG).
The
ANG
is
dense
network
epithelium-lined
tubules,
some
which
are
dominated
by
single
taxon.
These
deposited
into
squid
eggs,
where
they
defend
developing
embryos
from
harmful
biofouling.
This
study
combination
visualize
different
dimensions
its
communities.
Imaging
entire
organs
sheet
microscopy
revealed
that
composite
tissue
individual,
non-intersecting
tubules
each
their
own
population.
bisected,
converging
towards
two
points
organ.
At
these
points,
empty
space
can
mix
jelly
be
onto
eggs.
Observations
populations
correlated
taxa
cell
morphology
show
tubule
varied:
contained
mixed
phyla
while
only
one
genus
bacteria.
Together,
data
shed
on
interact
within
uses
physical
maintain
employ
population
defensive
context.
IMPORTANCE
Sequence-based
microbiome
studies
have
much
about
hosts
communities
microbiota,
but
often
lack
spatial
understanding
microbes
relate
other
reside.
reveal
houses
diverse,
beneficial
deploys
them
for
egg
defense.
findings
suggest
partitioning
may
key
harboring
diverse
antimicrobial-producing
establish
foundation
further
structures
mediate
interactions.
Язык: Английский
Variance inC. elegansgut bacterial load suggests complex host-microbe dynamics
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 12, 2024
Abstract
Variation
in
bacterial
composition
inside
a
host
is
result
of
complex
dynamics
microbial
community
assembly,
but
little
known
about
these
dynamics.
To
deconstruct
the
factors
that
contribute
to
this
variation,
we
used
combination
experimental
and
modeling
approaches.
We
found
demographic
stochasticity
stationary
heterogeneity
carrying
capacity
or
growth
rate
are
insufficient
explain
quantitatively
variation
observed
our
empirical
data.
Instead,
data
can
be
understood
if
host-bacteria
system
viewed
as
stochastically
switching
between
high
low
rates
phenotypes.
This
suggests
significantly
more
than
logistic
canonical
models
microbiome
assembly.
develop
mathematical
process
various
aspects
highlight
limitations
snapshot
describing
host-associated
communities
importance
using
time-series
along
with
understand
within
host.
Язык: Английский