Spatiotemporal properties of glutamate input support direction selectivity in the dendrites of retinal starburst amacrine cells DOI Creative Commons
Prerna Srivastava, Geoff deRosenroll, Benjamin L. Murphy‐Baum

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Июль 13, 2022

A bstract The asymmetric summation of kinetically distinct glutamate inputs across the dendrites retinal “starburst” amacrine cells is proposed to underlie their direction selective properties, but experimentally verifying input kinetics has been a challenge. Here, we used two-photon sensor (iGluSnFR) imaging directly measure individual starburst dendrites. We found that signals measured from proximal were relatively sustained compared those distal These differences observed range stimulus sizes and appeared be shaped mainly by excitatory rather than inhibitory network interactions. Temporal deconvolution analysis suggests steady-state vesicle release rate was ∼ 3 times larger at sites sites. Using connectomics-inspired computational model, demonstrate play an important role in shaping selectivity low velocities. Together, these results provide direct support for ‘space-time wiring’ model selectivity.

Язык: Английский

Origins of direction selectivity in the primate retina DOI Creative Commons
Yeon Jin Kim,

Beth B. Peterson,

Joanna D. Crook

и другие.

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Май 23, 2022

From mouse to primate, there is a striking discontinuity in our current understanding of the neural coding motion direction. In non-primate mammals, directionally selective cell types and circuits are signature feature retina, situated at earliest stage visual process. primates, by contrast, direction selectivity hallmark processing areas cortex, but has not been found despite significant effort. Here we combined functional recordings light-evoked responses connectomic reconstruction identify diverse direction-selective macaque monkey retina with distinctive physiological properties synaptic motifs. This circuitry includes an ON-OFF ganglion type, spiking, polyaxonal amacrine starburst cell, all which show selectivity. Moreover, discovered that cells possess strong, non-GABAergic, antagonistic surround mediated input from excitatory bipolar critical for generation radial sensitivity these cells. Our findings open door investigation precortical computes primate system.

Язык: Английский

Процитировано

51
Spatiotemporal properties of glutamate input support direction selectivity in the dendrites of retinal starburst amacrine cells DOI Creative Commons
Prerna Srivastava,

Geoff de Rosenroll,

Akihiro Matsumoto

и другие.

eLife, Год журнала: 2022, Номер 11

Опубликована: Ноя. 8, 2022

The asymmetric summation of kinetically distinct glutamate inputs across the dendrites retinal ‘starburst’ amacrine cells is one several mechanisms that have been proposed to underlie their direction-selective properties, but experimentally verifying input kinetics has a challenge. Here, we used two-photon sensor (iGluSnFR) imaging directly measure individual starburst dendrites. We found signals measured from proximal were relatively sustained compared those distal These differences observed range stimulus sizes and appeared be shaped mainly by excitatory rather than inhibitory network interactions. Temporal deconvolution analysis suggests steady-state vesicle release rate was ~3 times larger at sites sites. Using connectomics-inspired computational model, demonstrate play an important role in shaping direction selectivity low velocities. Taken together, these results provide direct support for ‘space-time wiring’ model selectivity.

Язык: Английский

Процитировано

25
Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Open Access

John A. Gaynes,

Samuel A. Budoff,

Michael J. Grybko

и другие.

Опубликована: Сен. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Язык: Английский

Процитировано

1
Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Creative Commons

John A. Gaynes,

Samuel A. Budoff,

Michael J. Grybko

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Сен. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties BCs along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity (DS) when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar-SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal DS are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation SACs.

Язык: Английский

Процитировано

1
Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Creative Commons

John A. Gaynes,

Samuel A. Budoff,

Michael J. Grybko

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 5, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar-SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Язык: Английский

Процитировано

0
Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Open Access

John A. Gaynes,

Samuel A. Budoff,

Michael J. Grybko

и другие.

Опубликована: Дек. 5, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF- murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Язык: Английский

Процитировано

0
Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Creative Commons

John A. Gaynes,

Samuel A. Budoff,

Michael J. Grybko

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Дек. 27, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties BCs along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity (DS) when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal DS are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation SACs.

Язык: Английский

Процитировано

0
eLife assessment: Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Open Access
Marla B. Feller

Опубликована: Сен. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Язык: Английский

Процитировано

0
Reviewer #3 (Public Review): Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells DOI Open Access

John A. Gaynes,

Samuel A. Budoff,

Michael J. Grybko

и другие.

Опубликована: Сен. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Язык: Английский

Процитировано

0
Spatiotemporal properties of glutamate input support direction selectivity in the dendrites of retinal starburst amacrine cells DOI Creative Commons
Prerna Srivastava, Geoff deRosenroll, Benjamin L. Murphy‐Baum

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Июль 13, 2022

A bstract The asymmetric summation of kinetically distinct glutamate inputs across the dendrites retinal “starburst” amacrine cells is proposed to underlie their direction selective properties, but experimentally verifying input kinetics has been a challenge. Here, we used two-photon sensor (iGluSnFR) imaging directly measure individual starburst dendrites. We found that signals measured from proximal were relatively sustained compared those distal These differences observed range stimulus sizes and appeared be shaped mainly by excitatory rather than inhibitory network interactions. Temporal deconvolution analysis suggests steady-state vesicle release rate was ∼ 3 times larger at sites sites. Using connectomics-inspired computational model, demonstrate play an important role in shaping selectivity low velocities. Together, these results provide direct support for ‘space-time wiring’ model selectivity.

Язык: Английский

Процитировано

0