Young transposable elements rewired gene regulatory networks in human and chimpanzee hippocampal intermediate progenitors DOI Open Access
Sruti Patoori, Samantha M. Barnada, Christopher R. L. Large

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2021, Номер unknown

Опубликована: Ноя. 24, 2021

Abstract The hippocampus is associated with essential brain functions such as learning and memory. Human hippocampal volume significantly greater than expected when compared to non-human apes, suggesting a recent expansion. Intermediate progenitors, which are able undergo multiple rounds of proliferative division before final neurogenic division, may have played role in the evolutionary To investigate evolution gene regulatory networks underpinning neurogenesis we leveraged differentiation human chimpanzee induced Pluripotent Stem Cells into TBR2-positive intermediate progenitors (hpIPCs). We find that active hpIPCs different between humans chimpanzees, ∼2,500 genes differentially expressed. demonstrate species-specific transposon-derived enhancers contribute these transcriptomic differences. Young transposons, predominantly Endogenous Retroviruses (ERVs) SINE-Vntr-Alus (SVAs), were co-opted manner. Human-specific SVAs provided substrates for thousands novel TBR2 binding sites, CRISPR-mediated repression attenuates expression ∼25% upregulated relative same cell population chimpanzee. Summary statement Evolution development was mediated by co-option young retrotransposons enhancers.

Язык: Английский

Young transposable elements rewired gene regulatory networks in human and chimpanzee hippocampal intermediate progenitors DOI Open Access
Sruti Patoori, Samantha M. Barnada, Christopher R. L. Large

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2021, Номер unknown

Опубликована: Ноя. 24, 2021

Abstract The hippocampus is associated with essential brain functions such as learning and memory. Human hippocampal volume significantly greater than expected when compared to non-human apes, suggesting a recent expansion. Intermediate progenitors, which are able undergo multiple rounds of proliferative division before final neurogenic division, may have played role in the evolutionary To investigate evolution gene regulatory networks underpinning neurogenesis we leveraged differentiation human chimpanzee induced Pluripotent Stem Cells into TBR2-positive intermediate progenitors (hpIPCs). We find that active hpIPCs different between humans chimpanzees, ∼2,500 genes differentially expressed. demonstrate species-specific transposon-derived enhancers contribute these transcriptomic differences. Young transposons, predominantly Endogenous Retroviruses (ERVs) SINE-Vntr-Alus (SVAs), were co-opted manner. Human-specific SVAs provided substrates for thousands novel TBR2 binding sites, CRISPR-mediated repression attenuates expression ∼25% upregulated relative same cell population chimpanzee. Summary statement Evolution development was mediated by co-option young retrotransposons enhancers.

Язык: Английский

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