Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
rapid
intracellular
calcium
increase,
phosphorylation
of
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
The
insect
gut
microbiota
forms
a
complex,
multifunctional
system
that
significantly
affects
phenotypic
traits
linked
to
environmental
adaptation.
Strong
reproductive
potential
underpins
the
migratory
success,
population
growth
and
destructive
impact
of
fall
armyworm,
Spodoptera
frugiperda
(J.E.
Smith).
However,
precise
role
bacteria
in
S.
processes,
distribution
transmission
dynamics
remains
unclear.
We
examined
major
invasive
agricultural
pest,
identifying
Enterococcus,
Enterobacter,
Klebsiella
as
core
microorganisms
present
throughout
its
life
cycle.
These
microbes
showed
heightened
activity
during
egg
stage,
early
larval
stages
pre-oviposition
period
females.
Using
an
axenic
re-infection
system,
Enterococcus
quebecensis
FAW181,
michiganensis
FAW071
Enterobacter
hormaechei
FAW049
were
found
enhance
host
fecundity,
increasing
production
by
62.73%,
59.95%,
56.71%,
respectively.
Metagenomic
haemolymph
metabolomic
analyses
revealed
positive
correlation
between
symbiotic
hormone
metabolism
female
frugiperda.
Further
analysis
metabolites
biosynthesis
pathway,
along
with
exogenous
injection
juvenile
III
20-hydroxyecdysone,
regulate
these
hormones,
maintaining
levels
equivalent
those
control
insects.
This
regulation
supports
improved
fecundity
frugiperda,
aiding
rapid
colonization
expansion.
findings
emphasize
pivotal
E.
FAW049,
K.
enhancing
reproduction
modulating
JH
through
JHAMT
concurrently
20E
precursors
via
PHM.
Our
results
provide
novel
insights
into
microbe-host
symbiosis
pest
management
strategies
for
alien
species.
Video
Abstract.
Insect
fat
body,
a
central
tissue
for
nutrient
storage,
energy
metabolism,
and
protein
synthesis,
degrades
by
apoptosis
autophagy
during
larval
metamorphosis.
After
adult
emergence,
the
body
grows
rapidly
with
cell
proliferation
polyploidization
previtellogenic
period
but
ceases
in
vitellogenic
phase.
So
far,
regulatory
mechanisms
underlying
fate
decisions
adulthood
remain
unknown.
Transcriptomic
analysis
of
locust
revealed
enrichment
pathways
associated
cycle,
nuclear
division,
DNA
replication.
Decapentaplegic
(Dpp)
was
among
top
differentially
expressed
genes
signaling
cascades
involved
regulating
proliferation.
Abundance
Dpp,
phosphorylated
Mad
(p-Mad),
Medea
increased
stage
subsequently
declined
Knockdown
Mad,
resulted
suppressed
proliferation,
along
remarkably
reduced
number
blocked
vitellogenin
(Vg)
expression
as
well
consequent
arrest
egg
development.
Mad/Medea
complex
bound
to
promoters
cyclin
B
(CycB)
polo-like
kinase
1
(Plk1)
stimulated
their
expression.
Depletion
CycB
Plk1
caused
defective
phenotypes
resembling
knockdown.
In
phase,
high
levels
juvenile
hormone
(JH)
promoted
degradation
via
fizzy-related
(Fzr)-mediated
ubiquitination,
leading
inhibited
The
results
suggest
that
development
is
bone
morphogenetic
(BMP)
pathway,
whereas
JH
antagonize
BMP
ceasing
findings
provide
novel
insights
into
regulation
transition
growth
Vg
synthesis
reproductive
requirements.
Ecotoxicology and Environmental Safety,
Год журнала:
2023,
Номер
257, С. 114951 - 114951
Опубликована: Апрель 26, 2023
Modern
agriculture
has
many
environmental
consequences,
such
as
soil
contamination,
accumulation
of
toxic
compounds
in
the
environment
or
risk
adverse
effects
on
nontarget
organisms
and
for
these
reasons,
scientists
are
seeking
a
more
environmentally
friendly
alternative
to
synthetic
insecticides.
This
study
investigated
four
plant
secondary
metabolites
classified
volatile
organic
(VOCs),
which
have
potential
bioinsecticides,
(E)-2-decenal,
furfural,
2-undecanone
(E,E)-2-4-decadienal,
concentrations
10-5
10-7
M,
female
reproductive
processes
larval
hatchability
Tenebrio
molitor
beetle.
Our
indicates
proper
development
ovaries
after
application
however
volume
terminal
oocytes
was
significantly
reduced,
with
strongest
effect
(E)-
2-decenal
reduced
approximately
three
times.
The
relative
vitellogenin
expression
level
observed
(E,E)-
2-4-decadienal
concentration
at
same
time
patency
index
up
2-times
furfural
M.
What
is
important
morphological
changes
translated
into
physiological
ones.
number
laid
eggs
affected,
inhibition
(∼43%
reduction),
(∼33%)
M
(∼33%).
Moreover,
we
13%
(in
case
2-undecanone)
decrease
hatchability.
Tested
exhibited
repellent
caused
60%
reduction
insect
survivability
Altogether,
VOCs
seems
like
bioactive
protection.
Abstract
Background
Juvenile
hormones
(JH)
play
crucial
role
in
regulating
development
and
reproduction
insects.
The
most
common
form
of
JH
is
III,
derived
from
MF
through
epoxidation
by
CYP15
enzymes.
However,
the
higher
dipterans,
such
as
fruitfly,
Drosophila
melanogaster
,
a
bis-epoxide
JHB3,
accounted
detected.
Moreover,
these
dipterans
have
lost
gene
their
genomes.
As
result,
identity
P450
epoxidase
biosynthesis
pathway
remains
unknown.
Results
In
this
study,
we
show
that
Cyp6g2
serves
major
responsible
for
JHB3
III
D.
.
predominantly
expressed
corpus
allatum
(CA),
concurring
with
expression
pattern
jhamt
another
well-studied
last
steps
biosynthesis.
Mutation
leads
to
severe
disruptions
larval-pupal
metamorphosis
exhibits
reproductive
deficiencies,
exceeding
those
seen
mutants.
Notably,
−/−
::jhamt
2
double
mutants
all
died
at
pupal
stage
but
could
be
rescued
topical
application
analogs.
titer
analyses
revealed
both
mutant
lacking
while
overexpression
or
caused
significant
increase
titer.
Conclusions
These
findings
collectively
established
laid
groundwork
further
understanding
pave
way
developing
specific
inhibitors
insect
growth
regulators
insecticides.
The
halophilic
zooplankton
brine
shrimp
Artemia
has
been
used
as
an
experimental
animal
in
multidisciplinary
studies.
However,
the
reproductive
patterns
and
its
regulatory
mechanisms
remain
unclear.
In
this
study,
ovarian
development
process
of
parthenogenetic
(A.
parthenogenetica)
was
divided
into
five
stages,
oogenesis
or
egg
formation
identified
six
phases.
mode
assumed
to
be
polytrophic.
We
also
traced
dynamic
translocation
candidate
germline
stem
cells
(cGSCs)
using
EdU
labelling
elucidated
several
key
cytological
events
through
haematoxylin
eosin
staining
fluorescence
imaging.
Distinguished
from
ovary
structure
insects
crustaceans,
germarium
originated
ovariole
buds
are
located
at
base
ovarioles.
RNA-seq
based
on
stages
2657
upregulated
genes
related
reproduction
by
pair-to-pair
comparison.
Gbb,
Dpp,
piwi,
vasa,
nanos,
VgA
VgR
associated
with
cGSCs
recognition
were
screened
verified
qPCR.
Silencing
gene
A.
parthenogenetica
(Ap-VgR)
Stage
II
led
a
low
level
expression
(less
than
10%)
within
5
days,
which
resulted
variations
oogenesis-related
significantly
inhibited
vitellogenesis,
impeded
oocyte
maturation,
eventually
decreased
number
offspring.
conclusion,
we
have
illustrated
development,
outlined
spatio-temporal
features
negative
impacts
knockdown
animal.
findings
study
lay
foundation
for
further
biology
invertebrates.
Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
rapid
intracellular
calcium
increase,
phosphorylation
of
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
Journal of Agricultural and Food Chemistry,
Год журнала:
2022,
Номер
71(1), С. 300 - 310
Опубликована: Дек. 20, 2022
The
insulin
receptor
substrate
(IRS),
as
the
core
cytoplasmic
adapter
protein
in
insulin/insulin-like
signaling
(IIS)
pathway,
is
an
important
mediator
of
cellular
signaling.
However,
it
still
unknown
how
IRS
crosstalk
with
hormone
regulates
insect
growth,
development,
and
reproduction.
In
this
study,
we
demonstrated
that
knockdown
IRS1
significantly
inhibited
oogenesis,
vitellogenesis,
development
nurse
cells
follicular
epithelial
cells.
addition,
qRT-PCR
results
showed
FOXO
transcription
factors
responded
to
silencing
gene.
had
no
significant
effect
on
expression
juvenile
hormone/20-hydroxyecdysone
(JH/20E)-signaling
genes,
JH
synthesis,
degradation
enzyme-related
genes
JH/20E
titers.
Our
suggested
IIS
pathway
regulated
ovarian
Vg
production
through
FOXO,
independent
20E
pathways.
This
study
revealed
reproductive
regulation
mechanism
Propylea
japonica,
which
provides
a
theoretical
basis
for
large-scale
expansion
P.
japonica
environment-friendly
biological
control
strategy.
Parasites & Vectors,
Год журнала:
2023,
Номер
16(1)
Опубликована: Сен. 13, 2023
Abstract
Background
Female
mosquitoes
need
a
blood
meal
after
mating
for
their
eggs
to
develop,
and
this
behavior
leads
the
spread
of
pathogens.
Therefore,
understanding
molecular
regulation
reproduction
in
female
is
essential
control
mosquito
vector
populations.
In
study,
we
reported
that
microRNA-989
(miR-989),
which
targets
5-HTR1
(encoding
secreted
5-hydroxytryptamine
receptor1
),
reproduction.
Methods
The
spatiotemporal
expression
profile
miR-989
was
detected
using
quantitative
real-time
reverse
transcription
PCR
(RT-qPCR).
antagomirs
antagomir-negative
(NC)
were
designed
synthesized
knock
down
endogenous
mosquitoes.
RNA
sequencing
used
analyze
ovarian
response
deletion.
predicted
confirmed
RNAhybrid
dual-luciferase
assays.
Results
exclusively
expressed
ovaries
responds
feeding.
Injection
antagomir
resulted
smaller
reduced
egg
production.
demonstrated
as
target
miR-989.
deletion
contributed
upregulation
expression.
Knockdown
rescued
adverse
production
caused
by
silencing.
Thus,
might
play
an
role
targeting
.
Conclusions
We
found
participates
These
findings
expand
our
reproduction-related
miRNAs
promote
new
strategies
Graphical
