A gapless genome sequence of the fungus Botrytis cinerea

Molecular Plant Pathology, Год журнала: 2016, Номер 18(1), С. 75 - 89

Опубликована: Фев. 26, 2016

Following earlier incomplete and fragmented versions of a genome sequence for the grey mould Botrytis cinerea, gapless, near-finished B. cinerea strain B05.10 is reported. The assembly comprised 18 chromosomes was confirmed by an optical map genetic based on approximately 75 000 single nucleotide polymorphism (SNP) markers. All contained fully assembled centromeric regions, 10 had telomeres both ends. consisted 4153 cM comparison distances with physical identified 40 recombination hotspots. linkage also two mutations, located in previously described genes Bos1 BcsdhB, that conferred resistance to fungicides boscalid iprodione. predicted encode 11 701 proteins. RNAseq data from >20 different samples were used validate improve gene models. Manual curation chromosome 1 revealed interesting features, such as occurrence dicistronic transcript overlapping opposite orientations, well many spliced antisense transcripts. untranslated regions (UTRs) can be complex long, UTRs exceeding lengths kb possessing multiple introns. Community annotation progress.

Язык: Английский

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Many Shades of Grey in Botrytis–Host Plant Interactions

Trends in Plant Science, Год журнала: 2018, Номер 23(7), С. 613 - 622

Опубликована: Апрель 30, 2018

Язык: Английский

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Lytic Polysaccharide Monooxygenases: The Microbial Power Tool for Lignocellulose Degradation

Trends in Plant Science, Год журнала: 2016, Номер 21(11), С. 926 - 936

Опубликована: Авг. 13, 2016

Язык: Английский

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Molecular aspects in pathogen-fruit interactions: Virulence and resistance

Postharvest Biology and Technology, Год журнала: 2016, Номер 122, С. 11 - 21

Опубликована: Май 1, 2016

Язык: Английский

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Circadian rhythms: pervasive, and often times evasive

Philosophical Transactions of the Royal Society B Biological Sciences, Год журнала: 2025, Номер 380(1918)

Опубликована: Янв. 23, 2025

Most circadian texts begin by stating that clocks are pervasive throughout the tree of life. Indeed, clock mechanisms have been described from cyanobacteria to humans, representing a notable example convergent evolution: yet, there several phyla in animals, protists or within fungi and bacteria, which homologs some-or all-known components seem be absent, posing inevitable questions about evolution systems. Moreover, as we move away model organisms, taxa core elements can identified at genomic levels. However, functional description those putative has hard achieve, rhythmicity is not observed unless defined abiotic nutritional cues provided. The 'conditioning' functionality remain uncertain, emphasizing need delve further into non-model As absence evidence absence, lack known core-clock observable rhythms given organism, cannot an priori criterion discard presence clock, may limited yet untested experimental conditions phenotypes. This article seeks reflect on these topics, highlighting some pressing awaiting addressed.This part Theo Murphy meeting issue 'Circadian infection immunity'.

Язык: Английский

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Stress tolerance and virulence of insect-pathogenic fungi are determined by environmental conditions during conidial formation

Current Genetics, Год журнала: 2015, Номер 61(3), С. 383 - 404

Опубликована: Март 19, 2015

Язык: Английский

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DHN melanin biosynthesis in the plant pathogenic fungus Botrytis cinerea is based on two developmentally regulated key enzyme (PKS)‐encoding genes

Molecular Microbiology, Год журнала: 2015, Номер 99(4), С. 729 - 748

Опубликована: Окт. 30, 2015

Summary B otrytis cinerea is the causal agent of gray mold disease in various plant species and produces grayish macroconidia and/or black sclerotia at end infection cycle. It has been suggested that pigmentation due to accumulation 1,8‐dihydroxynaphthalene ( DHN ) melanin. To unravel its basis regulation, putative melanogenic regulatory genes were identified functionally characterized. Unlike other melanin‐producing fungi, . Leotiomycetes contain two key enzyme PKS )‐encoding enzymes. Bcpks12 bcpks13 are developmentally regulated required for melanogenesis conidia respectively. BcYGH 1 converts BcPKS13 product contributes thereby conidial melanogenesis. In contrast, enzymes acting downstream conversion products BcBRN 2, BcSCD 1) both, sclerotial melanogenesis, suggesting follows a non‐linear pathway rather unusual secondary metabolic pathways. Regulation involves three pathway‐specific transcription factors TFs clustered with bcpks12 or developmental regulators such as light‐responsive Melanogenic dispensable vegetative mycelia proper growth virulence. However, melanin considered contribute longevity reproduction structures.

Язык: Английский

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How light affects the life of Botrytis

Fungal Genetics and Biology, Год журнала: 2017, Номер 106, С. 26 - 41

Опубликована: Июнь 23, 2017

Fungi, like other organisms, actively sense the environmental light conditions in order to drive adaptive responses, including protective mechanisms against light-associated stresses, and regulate development. Ecological niches are characterized by different regimes, for instance is absent underground, spectra from sunlight changed underwater or under canopy of foliage due absorption distinct wavelengths bacterial, algal plant pigments. Considering fact that fungi have evolved adapt their habitats, complexities 'visual' systems may vary significantly. Fungi pathogenic on plants experience a special regime because host always seeks optimum photosynthesis – pathogen has cope with this environment. When lives indirectly exposed sunlight, it confronted an altered spectrum enriched green far-red light. Botrytis cinerea, gray mold fungus, aggressive mainly infecting above-ground parts plant. As outlined review, Leotiomycete maintains highly sophisticated signaling machinery, integrating (near)-UV, blue, green, red signals use at least eleven potential photoreceptors trigger variety i.e. protection (pigmentation, enzymatic systems), morphogenesis (conidiation, apothecial development), entrainment circadian clock, positive negative tropism multicellular (conidiophores, apothecia) unicellular structures (conidial germ tubes). In sense, 'looking through eyes' will expand our knowledge fungal photobiology.

Язык: Английский

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A circadian oscillator in the fungus Botrytis cinerea regulates virulence when infecting Arabidopsis thaliana

Proceedings of the National Academy of Sciences, Год журнала: 2015, Номер 112(28), С. 8744 - 8749

Опубликована: Июнь 29, 2015

Significance Circadian clocks are molecular machineries that allow organisms to anticipate daily cyclic challenges and temporally modulate different processes. Thus, plant defense mechanisms against pathogens have been reported vary in Arabidopsis thaliana . Although the plant–pathogen interaction is a two-sided story, nothing known regarding circadian regulation of pathogenic traits. Herein we characterize functional clock necrotrophic fungal pathogen Botrytis cinerea , postulating additional roles for BcFRQ1, ortholog core component FREQUENCY Neurospora crassa By using clock-null mutants, demonstrate between this its host varies with time day, being B. key regulating outcome.

Язык: Английский

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Fungal photobiology: visible light as a signal for stress, space and time

Current Genetics, Год журнала: 2014, Номер 61(3), С. 275 - 288

Опубликована: Окт. 16, 2014

Язык: Английский

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