Quantification of dose-mortality responses in adult Diptera: Validation using Ceratitis capitata and Drosophila suzukii responses to spinosad

PLoS ONE, Год журнала: 2019, Номер 14(2), С. e0210545 - e0210545

Опубликована: Фев. 7, 2019

Quantitative laboratory bioassay methods are required to evaluate the toxicity of novel insecticidal compounds for pest control and determine presence resistance traits. We used a radioactive tracer based on 32P-ATP estimate volume droplet ingested by two dipteran pests: Ceratitis capitata (Tephritidae) Drosophila suzukii (Drosophilidae). Using blue food dye it was possible distinguish between individuals that solution from those did not. The average C. adults 1.968 μl. Females ~20% greater than males. Adults D. an 0.879 μl females ~30% feeding method validated using naturally-derived insecticide spinosad as active ingredient (a.i.). For capitata, concentration-mortality response not differ sexes or among three different batches insects. Lethal dose values were calculated mean volumes. LD50 1.462 1.502 ng a.i./insect males females, respectively, equivalent 0.274 0.271 a.i./mg when sex-specific variation in body weight considered. same process suzukii, value estimated at 2.927 a.i./insect, 1.994 1.67 mg both together. conclude this technique could be readily employed determination status dose-mortality responses many species pestiferous Diptera.

Язык: Английский

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Raspberry as a Source for the Development of Drosophila suzukii Attractants: Laboratory and Commercial Polytunnel Trials

Insects, Год журнала: 2019, Номер 10(5), С. 137 - 137

Опубликована: Май 10, 2019

Several commercial products and home-made attractants have been developed for monitoring mass-trapping of the spotted wing drosophila, Drosophila suzukii. Growers in Mexico adopted an attractant based on a fermenting mixture raspberry pulp sucrose, with anecdotally promising results. We compared capture rates traps baited + sucrose captures from range alternative attractants. Raspberry alone or was more attractive than apple cider vinegar (ACV) SuzukiiTrap similar to baker's yeast laboratory cage studies. Synthetic aroma (0.1-10% concentration), water mixed ACV, did not improve laboratory. Traps ACV had D. suzukii blackberry polytunnels Michoacán, Mexico. captured significantly higher numbers other drosophilid species, leading total flies (all species), which may explain why Mexican growers favor raspberry-based attractant. The Z-Kinol lower polytunnels, although highest selectivity (81% captured). A two-component trap (2C trap) ethanol as drowning solution ventilated tube device markedly effective currently used by growers. conclude that is attraction under polytunnel conditions. 2C performed better transparent cup berry producers

Язык: Английский

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Marking Drosophila suzukii (Diptera: Drosophilidae) with Fluorescent Dusts

Insects, Год журнала: 2020, Номер 11(3), С. 152 - 152

Опубликована: Март 1, 2020

The marking of Drosophila suzukii can be an important instrument for studying the ecology and behaviour this economically fruit pest, aiding development new Integrated Pest Management (IPM) tools or strategies. There is, however, a need cost-effective methodology that provides easily detectable stable mark. Whereas fluorescent pigment powders are often used in entomological research, pigments (series, dyes), application techniques, doses to evaluated each studied species terms their efficacy possible adverse effects on performance insect. effectiveness different techniques dyes (RadGlo® TP-series) effect survival adult D. were investigated laboratory. Furthermore, influence flies was examined laboratory trap assays (olfaction) field recapture study (general orientation). persistence detectability marks both living (for techniques) dead under trapping/storage conditions. use mark yielded clearly highly persistent mark, without any flies.

Язык: Английский

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Olfactory Cues From Host- and Non-host Plant Odor Influence the Behavioral Responses of AdultDrosophila suzukii(Diptera: Drosophilidae) to Visual Cues

Environmental Entomology, Год журнала: 2021, Номер 50(3), С. 571 - 579

Опубликована: Янв. 20, 2021

Abstract While trapping methods for Drosophila suzukii Matsumura (Diptera: Drosophilidae) have typically relied on fermentation volatiles alone or in association with a visual stimulus, the relative contribution of and olfactory stimuli to food- host-seeking behavior D. is poorly understood. This study quantified type response exhibited by male female color effects that (fermentation, fresh fruit, leaf) exert outcome. Seven-, four- two-choice assays were used quantify interactions between cues. When no present seven-choice assay, preferred red, black, green pigments. Black red when yeast odors present, black was most attractive blueberry odor present. A strawberry leaf terpenoid, β-cyclocitral, seemed overridden flies’ color. In four-choice assays, more likely interact synergistically than β-cyclocitral. demonstrates modulates multimodal sensory modalities (vision olfaction) depending, some extent, stimuli. Our findings also provide insight into importance vision as function quality this invasive species.

Язык: Английский

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Selection of Lactic Acid Bacteria Species and Strains for Efficient Trapping of Drosophila suzukii

Insects, Год журнала: 2021, Номер 12(2), С. 153 - 153

Опубликована: Фев. 11, 2021

(1) Monitoring of Drosophila suzukii is based on the use effective traps and baits. The current baits are insufficient to provide efficient monitoring. bacteria as bio-catalyzers produce bioactive volatiles may improve flies’ attraction. Thus, we conducted this work Droskidrink® bait’s attractiveness using lactic acid bacteria. (2) Different that were assessed for attraction in a Droso-Trap® vineyard. Oenococcus oeni, Pediococcus spp., Lactobacillus spp. used. performance most attractive species, O. inoculated into was laboratory tests. responses female flies produced by with oeni strains recorded electroantennography. (3) Preliminary field assessment species. Three strain groups showed adaptation test conditions. Volatiles extracted headspace elicited electroantennographic from fly antennae. (4) highly bait These findings will be useful improving D. commercial utilization LAB strain-dependent manner.

Язык: Английский

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Efficacy of food-based attractants for monitoring Drosophila suzukii (Diptera: Drosophilidae) in berry crops

Crop Protection, Год журнала: 2021, Номер 150, С. 105797 - 105797

Опубликована: Авг. 23, 2021

Язык: Английский

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Drosophila suzukii in Argentina: new reports, review, and expansion of its distribution

Revista de la Sociedad Entomológica Argentina, Год журнала: 2023, Номер 82(01)

Опубликована: Март 31, 2023

Drosophila suzukii Matsumura, 1931 (Diptera: Drosophilidae) is an invasive pest species that causes damage to fruit crops due premature deterioration of ripe fruits by oviposition inside them. A review D. detections in Argentina was carried out and we updated this list with the results our sampling Northwest, Cuyo Northeast regions. The surveys determined first provinces Jujuy Córdoba, revealing continuous geographical expansion species.

Язык: Английский

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Canopy strip applications of Hanseniaspora uvarum combined with spinosad reduce insecticide use without compromising Drosophila suzukii control in cherry

Crop Protection, Год журнала: 2024, Номер 186, С. 106868 - 106868

Опубликована: Июль 31, 2024

Drosophila suzukii, a significant sweet cherry pest, has traditionally been controlled using broad-spectrum insecticides. Various yeasts are known to attract and stimulate flies feed on yeast-covered surfaces. We investigated an attract-and-kill formulation based the yeast Hanseniaspora uvarum insecticide spinosad, applied 80–100 cm wide canopy strip at 1-m height in six field trials across Italy Germany manage D. suzukii infestation cherry. The formulation, dose ranging from 21.6 34.9 g per hectare of according training system, decreased percentage infested cherries by 41.74–84.26% average number deposited eggs 79.51–95.56%. efficacy was comparable conventional application spinosad alone, which 84 216 hectare. 53.00–92.00% reduced 77.10–92.96%. technique led reduction up 90% use targeting limited section tree. targeted approach not only minimized quantities hectare, but also resulted lower residue levels treated fruits.

Язык: Английский

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Volatile Analysis of Sucrose-Grown Saccharomyces cerevisiae

ACS Agricultural Science & Technology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 25, 2024

Язык: Английский

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The Use of Novel Alginate Capsules in a Monitoring System for Drosophila suzukii in a Cherry Orchard in the Region of La Araucanía, Chile

Insects, Год журнала: 2024, Номер 16(1), С. 13 - 13

Опубликована: Дек. 27, 2024

Spotted wing drosophila (SWD) is a pest that causes damage due to the female laying eggs under skin of ripe fruit, from which larva emerges, causing its collapse and reducing commercial value. Due importance this pest, monitoring population starting point for any control program; however, there no early plan within management tasks, nor are studies on behavior, optimization traps, or their baits. This research proposes evaluation system with encapsulated baits adhesive traps allow effective control. The bait was selected after evaluating three options in olfactometric tests laboratory; most attractive WVM, 70% visits stimulus 30% control, unlike SAG I II, whose values did not exceed 40% attraction. Among expected results availability new format SWD better release rate over time, information obtained will generation curves area, essential decision-making. study contribute perspective nanomaterials, insect biology, agricultural entomology, monitoring.

Язык: Английский

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