Journal of Visualized Experiments,
Год журнала:
2021,
Номер
172
Опубликована: Июнь 14, 2021
Quantifying
food
intake
in
Drosophila
is
used
to
study
the
genetic
and
physiological
underpinnings
of
consumption-associated
traits,
their
environmental
factors,
toxicological
pharmacological
effects
numerous
substances.
Few
methods
currently
implemented
are
amenable
high
throughput
measurement.
The
Microplate
Feeder
Assay
(MFA)
was
developed
for
quantifying
consumption
liquid
individual
flies
using
absorbance.
In
this
assay,
consume
medium
from
select
wells
a
1536-well
microplate.
By
incorporating
dilute
tracer
dye
into
loading
known
volume
each
well,
absorbance
measurements
well
acquired
before
after
reflect
resulting
change
(i.e.,
consumed).
To
enable
analysis
with
method,
3D-printed
coupler
designed
that
allows
be
sorted
individually
96-well
microplates.
This
device
precisely
orients
96-
microplates
give
fly
access
up
4
consumption,
thus
enabling
preference
quantification
addition
regular
consumption.
Furthermore,
has
barrier
strips
toggle
between
open
closed
positions
allow
controlled
containment
release
column
samples
at
time.
method
enables
aqueous
solutions
by
many
simultaneously.
It
also
potential
adapted
other
insects
screen
nutrients,
toxins,
or
pharmaceuticals.
Journal of Applied Entomology,
Год журнала:
2024,
Номер
148(3), С. 339 - 350
Опубликована: Янв. 20, 2024
Abstract
Toxic
baits
comprising
a
combination
of
food
attractants
and
toxicant
could
contribute
to
the
control
spotted
wing
drosophila,
Drosophila
suzukii
(Diptera:
Drosophilidae),
major
invasive
pest
soft
fruit
berries.
Laboratory
cage
experiments
revealed
that
flies
both
sexes
were
significantly
more
attracted
dried
red
droplets
0.3%
sucrose
solution
likely
consume
red‐coloured
compared
blue,
green
or
colourless
droplets.
Flies
showed
tendency
feed
on
placed
floor
rather
than
presented
upside‐down
roof
side‐wall.
When
offered
commercial
insecticides
(5–50
parts
per
million
active
ingredient)
in
solution,
fly
mortality
was
highest
spinosad
treatment,
lowest
abamectin
intermediate
deltamethrin
spinetoram‐based
products.
Male
had
higher
females.
A
mixture
25
ppm
with
1.3%
+1.3%
corn
syrup
glycerol
(named
4%
mixture)
consumed
by
female
any
components
alone.
Addition
1%
apple
juice
resulted
an
additional
increase
spinosad‐induced
mortality.
In
contrast,
addition
cider
vinegar,
increasing
concentrations
(5%–10%),
yeasts
Saccharomyces
cerevisiae
Hanseniaspora
uvarum
(5
×
10
6
cells/mL)
did
not
for
reasons
unclear.
We
conclude
+1%
prove
be
useful
bait
delivery
other
biorational
D
.
control,
although
this
requires
field
testing
production
settings.
Journal of Visualized Experiments,
Год журнала:
2021,
Номер
172
Опубликована: Июнь 14, 2021
Quantifying
food
intake
in
Drosophila
is
used
to
study
the
genetic
and
physiological
underpinnings
of
consumption-associated
traits,
their
environmental
factors,
toxicological
pharmacological
effects
numerous
substances.
Few
methods
currently
implemented
are
amenable
high
throughput
measurement.
The
Microplate
Feeder
Assay
(MFA)
was
developed
for
quantifying
consumption
liquid
individual
flies
using
absorbance.
In
this
assay,
consume
medium
from
select
wells
a
1536-well
microplate.
By
incorporating
dilute
tracer
dye
into
loading
known
volume
each
well,
absorbance
measurements
well
acquired
before
after
reflect
resulting
change
(i.e.,
consumed).
To
enable
analysis
with
method,
3D-printed
coupler
designed
that
allows
be
sorted
individually
96-well
microplates.
This
device
precisely
orients
96-
microplates
give
fly
access
up
4
consumption,
thus
enabling
preference
quantification
addition
regular
consumption.
Furthermore,
has
barrier
strips
toggle
between
open
closed
positions
allow
controlled
containment
release
column
samples
at
time.
method
enables
aqueous
solutions
by
many
simultaneously.
It
also
potential
adapted
other
insects
screen
nutrients,
toxins,
or
pharmaceuticals.
PLoS ONE,
Год журнала:
2023,
Номер
18(9), С. e0286108 - e0286108
Опубликована: Сен. 28, 2023
The
medfly
Ceratitis
capitata
is
one
of
the
most
damaging
fruit
pests
with
quarantine
significance
due
to
its
extremely
wide
host
range.
use
entomopathogenic
fungi
constitutes
a
promising
approach
potential
applications
in
integrated
pest
management.
Furthermore,
developing
insect
control
methods
can
involve
fungal
machinery
cause
metabolic
disruption,
which
may
increase
effectiveness
by
impairing
development.
Insect
species,
including
C.
capitata,
relies
on
reproduction
potential,
nutrient
reserves,
activities,
and
immune
response
for
survival.
Accordingly,
purpose
this
study
was
investigate
impacts
fungus
Purpureocillium
lilacinum
pre-mortality.
V8
strain
subjected
laboratory
bioassays,
consisted
determining
virulence
P.
medfly.
applied
abdominal
topical
5-day-old
males
females.
Following
inoculation,
we
have
confirmed
(i)
significant
tissue
sugar
content,
(ii)
decrease
carbohydrase
digestive
glycosyl
hydrolase,
proteinase
activities
whole
midguts
treated
flies,
(iii)
antimicrobial
peptides
(AMPs)
genes
expression
profile
significantly
influenced
fly
gender,
status
(virgin,
mature,
mated),
time
after
infection,
but
infection
itself
had
no
discernible
impact
AMPs
that
were
examined.
This
provides
first
insight
into
how
could
affect
physiological
mechanisms
foundation
considering
as
novel,
biocontrol
agent.
Journal of Environmental Science,
Год журнала:
2019,
Номер
47(3), С. 47 - 64
Опубликована: Сен. 1, 2019
To
estimate
toxic
actions
of
two
insecticides
on
{lambda-cyhalothrin
(Karilot
gold
5%EC)
and
the
biopesticide
spinosad,
(Conserve
0.24%CB)},
adults
laboratory
strain
Mediterranean
fruit
fly,
Ceratitis
capitata
(Wied.)were
treated
with
different
concentrations.Discriminating
value
(LC99)
was
selected
for
monitoring
resistance
in
three
field
populations
collected
from
governorates
(Qaluobia,
Behira
Sharkia).Data
showed
that,
males
as
well
colony
strains
were
more
susceptible
than
females.Qaluobia
exhibited
highest
level
to
lambda-cyhalothrin
followed
by
Sharkia
colonies
both
male
female
flies
comparing
strain.Sharkia
remarkable
higher
spinosad
females
Qaluobia
colonies.On
other
hand,
remarked
levels
enzymes
activity
strain.Males
acid
phosphatase
Qaluobia,
whereas
females.The
alkaline
sexes.Behira
had
enzymatic
AChE
flies.The
total
protein
males,
but
case
flies.
Research Square (Research Square),
Год журнала:
2021,
Номер
unknown
Опубликована: Дек. 8, 2021
Abstract
Ceratitis
capitata
(medfly),
is
one
of
the
most
injurious
pests
fruits
with
quarantine
importance
because
its
extremely
wide
host
range.
The
use
entomopathogenic
fungi
constitutes
a
promising
approach
for
potential
applications
in
integrated
pest
management.
Nonetheless,
developing
methods
insect
control
can
also
involve
fungal
machinery
to
produce
metabolic
disturbance
that
increase
effectiveness
by
producing
detrimental
effect
on
development.
Insect
species,
such
as
capitata,
depend
reproduction
potential,
nutrient
reserves,
activities
and
immune
response
their
survival.
Accordingly,
purpose
this
study
fungus
Purpureocillium
lilacinum
investigate,
sublethal
effects
.
Laboratory
bioassays
were
conducted
medfly
V8
strain.
monitored
determine
virulence
P.
fruit
fly.
was
tested
against
5
days-old
males
females,
through
abdominal
topical
applications.
Following
inoculation,
we
showed
(i)
significant
sugar
amount
tissues,
(ii)
decrease
carbohydrase
activities,
digestive
glycosyl
hydrolase
proteinase
whole
midguts
treated
flies,
(iii)
an
over-expression
Takeout
Attacin-A
genes
induced
infection.
Moreover,
up-regulations
observed
relish
,
cecropin
1
ceratotox-A
defensin
are
due
physiological
mechanisms
occurring
during
Journal of Visualized Experiments,
Год журнала:
2021,
Номер
172
Опубликована: Июнь 14, 2021
Quantifying
food
intake
in
Drosophila
is
used
to
study
the
genetic
and
physiological
underpinnings
of
consumption-associated
traits,
their
environmental
factors,
toxicological
pharmacological
effects
numerous
substances.
Few
methods
currently
implemented
are
amenable
high
throughput
measurement.
The
Microplate
Feeder
Assay
(MFA)
was
developed
for
quantifying
consumption
liquid
individual
flies
using
absorbance.
In
this
assay,
consume
medium
from
select
wells
a
1536-well
microplate.
By
incorporating
dilute
tracer
dye
into
loading
known
volume
each
well,
absorbance
measurements
well
acquired
before
after
reflect
resulting
change
(i.e.,
consumed).
To
enable
analysis
with
method,
3D-printed
coupler
designed
that
allows
be
sorted
individually
96-well
microplates.
This
device
precisely
orients
96-
microplates
give
fly
access
up
4
consumption,
thus
enabling
preference
quantification
addition
regular
consumption.
Furthermore,
has
barrier
strips
toggle
between
open
closed
positions
allow
controlled
containment
release
column
samples
at
time.
method
enables
aqueous
solutions
by
many
simultaneously.
It
also
potential
adapted
other
insects
screen
nutrients,
toxins,
or
pharmaceuticals.
