Microbial functional assemblages predicted by the FAPROTAX analysis are impacted by physicochemical properties, but C, N and S cycling genes are not in mangrove soil in the Beibu Gulf, China

Ecological Indicators, Год журнала: 2022, Номер 139, С. 108887 - 108887

Опубликована: Апрель 22, 2022

Mangroves and their global significance in climate change based on the storage of blue carbon release CO2, CH4, N2O have attracted great attention. Microbial communities functions are essential for biogeochemical cycle (C), nitrogen (N), sulfur (S). Therefore, assessment microbial mangrove habitats, which suffer from different external environments, is necessary to evaluate unique ecological service functions. In present work, functional profiles four reserves (one sandy three muddy, Beibu Gulf, China) were detected by 16 s RNA gene amplicon-based Functional Annotation Procaryotic Taxa (FAPROTAX) analysis as well C, N, S q-PCR chips. The FAPROTAX showed that metabolic potential cycling indicated a difference regions. Moreover, some groups involved significantly correlated with physicochemical characteristics. However, genes chip did not same changes following variation environmental conditions those predicted analysis. Most equally distributed mangroves. Several N (amoA, amoB, nirK, nosZ), degradation (amyA, apu, mnp, lig, chiA), fixation (aclB), oxidation H2S (SoxY) higher sampling site Beihai City (Bei), was only soils. We hypothesized soil texture (sandy or muddy) rather than elements contributes more abundance S, C sediment. Our study demonstrated property easier modify genes. should pay attention redundancy microbiota when using microorganisms indicator access activity habitats.

Язык: Английский

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Analysis and Interpretation of metagenomics data: an approach

Biological Procedures Online, Год журнала: 2022, Номер 24(1)

Опубликована: Ноя. 19, 2022

Abstract Advances in next-generation sequencing technologies have accelerated the momentum of metagenomic studies, which is increasing yearly. The metagenomics field one versatile applications microbiology, where any interaction environment involving microorganisms can be topic study. Due to this versatility, number omics technology reached its horizons. Agriculture a crucial sector crop plants and interacting together. Hence, studying these interactions through lenses would completely disclose new meaning health development. rhizosphere an essential reservoir microbial community for agricultural soil. we focus on R&D studies crops such as rice, wheat, legumes, chickpea, sorghum. These recent developments are impossible without continuous advancement seen platforms; thus, brief introduction analysis available platforms presented here clear picture workflow. Concluding discussion about different pipelines applied analyze data produced by techniques significant role interpreting outcome particular experiment. A plethora software tools incorporated automated or individually perform manual analysis. Here describe 8–10 advanced, efficient used that explain their respective workflows simplify whole process.

Язык: Английский

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Zonation of bulk and rhizosphere soil bacterial communities and their covariation patterns along the elevation gradient in riparian zones of three Gorges reservoir, China

Environmental Research, Год журнала: 2024, Номер 249, С. 118383 - 118383

Опубликована: Фев. 7, 2024

Язык: Английский

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Spartina alterniflora invasion and mangrove restoration alter diversity and composition of sediment diazotrophic community

Applied Soil Ecology, Год журнала: 2022, Номер 177, С. 104519 - 104519

Опубликована: Май 7, 2022

Язык: Английский

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Chemoautotrophic sulphur oxidizers dominate microbial necromass carbon formation in coastal blue carbon ecosystems

Functional Ecology, Год журнала: 2023, Номер 37(10), С. 2634 - 2651

Опубликована: Авг. 24, 2023

Abstract Coastal blue carbon (C) ecosystems are recognized as efficient natural C sinks and play key roles in mitigating global climate change. Microbially driven C, nitrogen (N) sulphur (S) cycles crucial for ecosystem functioning, but how microorganisms drive sink formation sequestration coastal sediments remains unclear. In this study, we conducted a comprehensive analysis of amino sugars, N S cycling genes/pathways their associated taxa native ( Cyperus malaccensis Kandelia obovata ) alien Spartina alterniflora Sonneratia apetala vegetation. Compared to the alien‐vegetated sediment, native‐vegetated sediment had significantly p < 0.05) higher microbial necromass functional potentials chemoautotrophic fixation, degradation, methane cycling, 2 oxidation sulphate reduction. Also, our microbiomes showed that could be coupled with fixation and/or nitrate/nitrite oxidation, degradation were found pathways predicting C. Additionally, sulphur‐oxidizing Burkholderiales metagenome‐assembled genomes (MAGs) group dominated sediments. These results suggested oxidizers, particular novel lineage, might dominates through anabolism (C fixation);the coupling microbially processes; deposition derived This study provides insights into importance oxidizers shed new light on mechanism ecosystems, which also has important implications enhancing wetlands. Read free Plain Language Summary article Journal blog.

Язык: Английский

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Anthropogenic activities and geographic locations regulate microbial diversity, community assembly and species sorting in Canadian and Indian freshwater lakes

The Science of The Total Environment, Год журнала: 2022, Номер 826, С. 154292 - 154292

Опубликована: Март 4, 2022

Язык: Английский

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A Tight Interaction between the Native Seagrass Cymodocea nodosa and the Exotic Halophila stipulacea in the Aegean Sea Highlights Seagrass Holobiont Variations

Plants, Год журнала: 2023, Номер 12(2), С. 350 - 350

Опубликована: Янв. 11, 2023

Seagrasses harbour bacterial communities with which they constitute a functional unit called holobiont that responds as whole to environmental changes. Epiphytic rapidly respond both biotic and abiotic factors, potentially contributing the host fitness. The Lessepsian migrant Halophila stipulacea has high phenotypical plasticity harbours highly diverse epiphytic community, could support its invasiveness in Mediterranean Sea. current study aimed evaluate Halophila/Cymodocea competition Aegean Sea by analysing each of two seagrasses meadow zone where these intermingled, well their monospecific zones, at depths. Differences holobionts were evaluated using seagrass descriptors (morphometric, biochemical, elemental, isotopic composition) assess changes, 16S rRNA gene identify community structure composition. An Indicator Species Index was used bacteria significantly associated host. In mixed meadows, native C. nodosa shown be affected presence exotic H. stipulacea, terms plant communities, while responded only factors rather than proximity. This provided evidence competitive advantage on suggests possible use an ecological descriptor.

Язык: Английский

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Assembly processes underlying bacterial community differentiation among geographically close mangrove forests

mLife, Год журнала: 2023, Номер 2(1), С. 73 - 88

Опубликована: Март 1, 2023

Abstract Bacterial communities play pivotal roles in nutrient cycling mangrove forests. The assembly of microbial has been found to be influenced by complex factors, such as geographic distance, physicochemical conditions, and plant identity, but the relative importance these factors how shape assembling process remain elusive. We analyzed bacterial sampled from three species ( Aegiceras corniculatum , Bruguiera sexangula Kandelia obovata ) at locations along estuarine Dongzhai Harbor Hainan, China. revealed larger differences rhizosphere among geographical than species, indicated diversity, composition, interaction networks. that dispersal limitation homogeneous selection have substantial contributions all locations. Following phylogenetic‐bin‐based null model analysis (iCAMP) framework, we also showing dominance some bins. greater may mainly attributed proportions even a short distance. beta diversity was positively correlated with environmental distances, implying more similar conditions (such rich carbon nitrogen contents) shaped communities. concluded which are associated limitation, played key role communities, while identity contributed less.

Язык: Английский

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Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of Croceibacterium aestuarii sp. nov., a Novel Carotenoid-Producing Species in the Family Erythrobacteraceae

Journal of Marine Science and Engineering, Год журнала: 2024, Номер 12(1), С. 99 - 99

Опубликована: Янв. 3, 2024

Carotenoids are extensively used in drugs, cosmetics, nutrients, and foods, owing to their antioxidant anti-inflammatory characteristics. Diverse marine heterotrophic prokaryotes can accumulate carotenoids become promising alternatives for the advancement of production. In this research, 55 strains were isolated cultivated from tidal flat sediment Zhoushan classified into phyla Pseudomonadota (n = 24), Bacillota 18), Bacteroidota 9), Actinomycetota 4). Nine them accumulated carotenoids, most belonged families Flavobacteriaceae 4) Erythrobacteraceae Among those carotenoid-producing strains, one strain, designated as D39T, was proposed novel species belonging genus Croceibacterium through polyphasic taxonomy approaches. Genomic annotations carotenoid compound determinations revealed that strain D39T encoded crtEBIYZG genes mainly zeaxanthin major carotenoids. Furthermore, biosynthesis pathway majority identical with implying members be sources producing zeaxanthin. This study enhances knowledge microbial biodiversity flats, proposes a species, elucidates Croceibacterium, which contribute enriching promoting comprehensive insight genomic contents them.

Язык: Английский

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Uncovering potential mangrove microbial bioindicators to assess urban and agricultural pressures on Martinique island in the eastern Caribbean Sea

The Science of The Total Environment, Год журнала: 2024, Номер 928, С. 172217 - 172217

Опубликована: Апрель 6, 2024

Язык: Английский

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