Oncogenic signaling in the Drosophila prostate-like accessory gland activates a pro-tumorigenic program in the absence of proliferation
Samuel Jaimian Church,
Ajai J. Pulianmackal,
Joseph A. Dixon
и другие.
Disease Models & Mechanisms,
Год журнала:
2025,
Номер
18(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Drosophila
models
for
tumorigenesis
have
revealed
conserved
mechanisms
of
signaling
involved
in
mammalian
cancer.
Many
these
use
highly
mitotically
active
tissues.
Few
adult
tissues,
when
most
cells
are
terminally
differentiated
and
postmitotic.
The
accessory
glands
prostate-like
a
model
prostate
using
this
tissue
has
been
explored.
In
prior
model,
oncogenic
was
induced
during
the
proliferative
stages
gland
development,
raising
question
how
activity
impacts
differentiated,
postmitotic
tissue.
Here,
we
show
that
leads
to
activation
pro-tumorigenic
program,
similar
mitotic
but
absence
proliferation.
our
experiments,
led
hypertrophy
with
nuclear
anaplasia,
part
through
endoreduplication.
Oncogene-induced
gene
expression
changes
overlapped
those
polyploid
cancer
after
chemotherapy,
which
potentially
mediate
tumor
recurrence.
Thus,
provide
useful
aspects
progression
lack
cellular
Язык: Английский
Quantifying Transcriptome Turnover on Phylogenies by Modeling Gene Expression as a Binary Trait
Molecular Biology and Evolution,
Год журнала:
2025,
Номер
42(5)
Опубликована: Апрель 30, 2025
Abstract
Changes
in
gene
expression
are
a
key
driver
of
phenotypic
evolution,
leading
to
persistent
interest
the
evolution
transcriptomes.
Traditionally,
is
modeled
as
continuous
trait,
leaving
qualitative
transitions
largely
unexplored.
In
this
paper,
we
detail
development
new
Bayesian
inference
techniques
study
evolutionary
turnover
organ-specific
transcriptomes,
which
define
instances
where
orthologous
genes
gain
or
lose
particular
organ.
To
test
these
techniques,
analyze
transcriptomes
2
male
reproductive
organs,
testes
and
accessory
glands,
across
11
species
Drosophila
melanogaster
group.
We
first
discretize
states
by
estimating
probability
that
each
expressed
organ
species.
then
phylogenetic
model
correlated
transcriptome
more
organs
fit
it
state
data.
Inferences
under
imply
many
have
gained
lost
organ,
experienced
accelerated
on
different
branches
phylogeny.
Язык: Английский
Male Reproductive Glands and Their Secretions in Insects
Elsevier eBooks,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Post-eclosion growth in theDrosophilaEjaculatory Duct is driven by Juvenile Hormone signaling and is essential for male fertility
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 13, 2024
Abstract
The
Drosophila
Ejaculatory
duct
(ED)
is
a
secretory
tissue
of
the
somatic
male
reproductive
system.
ED
involved
in
secretion
seminal
fluid
components
and
ED-specific
antimicrobial
peptides
that
aid
fertility
female
post-mating
response.
composed
epithelial
cells
surrounded
by
layer
innervated
contractile
muscle.
grows
young
adult
males
during
first
24h
post-eclosion,
but
cell
cycle
status
role
post-eclosion
growth
have
been
unexplored.
Here,
we
show
undergo
variant
cycles
lacking
mitosis
called
endocycle,
lead
to
an
increase
organ
size
post
eclosion.
largely
exit
endocycle
day
3
adulthood,
when
ceases,
resulting
containing
ploidies
ranging
from
8C-32C.
directly
correlates
with
ploidy
cells,
additional
ectopic
endocycles
increasing
size.
When
endoreplication
compromised
it
leads
reduced
size,
protein
synthesis
fertility.
We
provide
evidence
endocycling
dependent
on
Juvenile
hormone
(JH)
signaling
suggest
hormone-induced
early
required
for
optimal
function
tissue.
propose
use
as
post-mitotic
model
study
polyploidy
regulating
function.
Язык: Английский
Quantifying transcriptome turnover on phylogenies by modeling gene expression as a binary trait
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 4, 2024
Abstract
Changes
in
gene
expression
are
a
key
driver
of
phenotypic
evolution,
leading
to
persistent
interest
the
evolution
transcriptomes.
Traditionally,
is
modeled
as
continuous
trait,
leaving
qualitative
transitions
largely
unexplored.
In
this
paper,
we
detail
development
new
Bayesian
inference
techniques
study
evolutionary
turnover
organ-specific
transcriptomes,
which
define
instances
where
orthologous
genes
gain
or
lose
particular
organ.
To
test
these
techniques,
analyze
transcriptomes
two
male
reproductive
organs,
testes
and
accessory
glands,
across
11
species
Drosophila
melanogaster
group.
We
first
discretize
states
by
estimating
probability
that
each
expressed
organ
species.
then
phylogenetic
model
correlated
transcriptome
more
organs
fit
it
state
data.
Inferences
under
show
many
have
gained
lost
organ,
experienced
accelerated
on
different
branches
phylogeny.
Язык: Английский
Post-eclosion growth in the Drosophila Ejaculatory Duct is driven by Juvenile Hormone signaling and is essential for male fertility
Developmental Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Oncogenic signaling in the adult Drosophila prostate-like accessory gland leads to activation of a conserved pro-tumorigenic program, in the absence of proliferation.
Samuel Jaimian Church,
Ajai J. Pulianmackal,
Joseph A. Dixon
и другие.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 12, 2024
Abstract
Drosophila
models
for
tumorigenesis
and
metastasis
have
revealed
conserved
mechanisms
of
signaling
that
are
also
involved
in
mammalian
cancer.
Many
these
use
the
proliferating
tissues
larval
stages
development,
when
highly
mitotically
active,
or
stem
cells
abundant.
Fewer
adult
animals
to
initiate
tumor
formation
many
largely
terminally
differentiated
postmitotic.
The
accessory
glands
prostate-like
a
model
some
aspects
prostate
using
this
tissue
has
been
explored.
In
model,
oncogenic
was
induced
during
proliferative
stage
gland
raising
question
how
activity
would
impact
postmitotic
tissue.
Here,
we
show
leads
activation
pro-tumorigenic
program,
similar
observed
mitotic
tissues,
but
absence
proliferation.
Oncogenic
hyperplasia
with
nuclear
anaplasia
aneuploidy
through
endoreduplication,
which
increases
polyploidy
occasionally
results
non-mitotic
neoplastic-like
extrusions.
We
compare
gene
expression
changes
our
endocycling
cancer
by
chemotherapy,
potentially
mediate
recurrence
after
treatment.
Similar
pathways
activated
cells,
suggesting
provide
useful
progression
do
not
involve
cellular
Язык: Английский