Oncogenic signaling in the Drosophila prostate-like accessory gland activates a pro-tumorigenic program in the absence of proliferation

Disease Models & Mechanisms, Journal Year: 2025, Volume and Issue: 18(4)

Published: April 1, 2025

ABSTRACT Drosophila models for tumorigenesis have revealed conserved mechanisms of signaling involved in mammalian cancer. Many these use highly mitotically active tissues. Few adult tissues, when most cells are terminally differentiated and postmitotic. The accessory glands prostate-like a model prostate using this tissue has been explored. In prior model, oncogenic was induced during the proliferative stages gland development, raising question how activity impacts differentiated, postmitotic tissue. Here, we show that leads to activation pro-tumorigenic program, similar mitotic but absence proliferation. our experiments, led hypertrophy with nuclear anaplasia, part through endoreduplication. Oncogene-induced gene expression changes overlapped those polyploid cancer after chemotherapy, which potentially mediate tumor recurrence. Thus, provide useful aspects progression lack cellular

Language: Английский

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Male Reproductive Glands and Their Secretions in Insects

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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Oncogenic signaling in the adult Drosophila prostate-like accessory gland leads to activation of a conserved pro-tumorigenic program, in the absence of proliferation.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 12, 2024

Abstract Drosophila models for tumorigenesis and metastasis have revealed conserved mechanisms of signaling that are also involved in mammalian cancer. Many these use the proliferating tissues larval stages development, when highly mitotically active, or stem cells abundant. Fewer adult animals to initiate tumor formation many largely terminally differentiated postmitotic. The accessory glands prostate-like a model some aspects prostate using this tissue has been explored. In model, oncogenic was induced during proliferative stage gland raising question how activity would impact postmitotic tissue. Here, we show leads activation pro-tumorigenic program, similar observed mitotic tissues, but absence proliferation. Oncogenic hyperplasia with nuclear anaplasia aneuploidy through endoreduplication, which increases polyploidy occasionally results non-mitotic neoplastic-like extrusions. We compare gene expression changes our endocycling cancer by chemotherapy, potentially mediate recurrence after treatment. Similar pathways activated cells, suggesting provide useful progression do not involve cellular

Language: Английский

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Post-eclosion growth in theDrosophilaEjaculatory Duct is driven by Juvenile Hormone signaling and is essential for male fertility

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 13, 2024

Abstract The Drosophila Ejaculatory duct (ED) is a secretory tissue of the somatic male reproductive system. ED involved in secretion seminal fluid components and ED-specific antimicrobial peptides that aid fertility female post-mating response. composed epithelial cells surrounded by layer innervated contractile muscle. grows young adult males during first 24h post-eclosion, but cell cycle status role post-eclosion growth have been unexplored. Here, we show undergo variant cycles lacking mitosis called endocycle, lead to an increase organ size post eclosion. largely exit endocycle day 3 adulthood, when ceases, resulting containing ploidies ranging from 8C-32C. directly correlates with ploidy cells, additional ectopic endocycles increasing size. When endoreplication compromised it leads reduced size, protein synthesis fertility. We provide evidence endocycling dependent on Juvenile hormone (JH) signaling suggest hormone-induced early required for optimal function tissue. propose use as post-mitotic model study polyploidy regulating function.

Language: Английский

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Quantifying transcriptome turnover on phylogenies by modeling gene expression as a binary trait

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

Abstract Changes in gene expression are a key driver of phenotypic evolution, leading to persistent interest the evolution transcriptomes. Traditionally, is modeled as continuous trait, leaving qualitative transitions largely unexplored. In this paper, we detail development new Bayesian inference techniques study evolutionary turnover organ-specific transcriptomes, which define instances where orthologous genes gain or lose particular organ. To test these techniques, analyze transcriptomes two male reproductive organs, testes and accessory glands, across 11 species Drosophila melanogaster group. We first discretize states by estimating probability that each expressed organ species. then phylogenetic model correlated transcriptome more organs fit it state data. Inferences under show many have gained lost organ, experienced accelerated on different branches phylogeny.

Language: Английский

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Post-eclosion growth in the Drosophila Ejaculatory Duct is driven by Juvenile Hormone signaling and is essential for male fertility

Developmental Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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