Cell Cycle,
Год журнала:
2023,
Номер
22(18), С. 1986 - 2002
Опубликована: Сен. 17, 2023
Transcription
is
a
crucial
stage
in
gene
expression.
An
integrated
study
of
34
RNA
polymerase
subunits
(RNAPS)
the
six
most
frequent
cancer
types
identified
several
genetic
and
epigenetic
modification.
We
discovered
nine
mutant
RNAPS
with
mutation
frequency
more
than
1%
at
least
one
tumor
type.
POLR2K
POLR2H
were
found
to
be
amplified
overexpressed,
whereas
POLR3D
was
deleted
downregulated.
Multiple
also
observed
regulated
by
variations
promoter
methylation.
5-Aza-2-deoxycytidine
mediated
re-expression
cell
lines
verified
methylation-driven
inhibition
POLR2F
POLR2L
expression
BRCA
NSCLC,
respectively.
Next,
we
showed
that
CD3EAP,
Pol
I
subunit,
overexpressed
all
associated
worst
survival
breast,
liver,
lung,
prostate
cancers.
The
knockdown
studies
CD3EAP
required
for
proliferation
induces
autophagy
but
not
apoptosis.
Furthermore,
rescued
cells.
correlated
S
G2
phase
cycle
regulators,
inhibited
CDK/cyclins.
POLR2D,
an
pol
II
as
commonly
prognostic
multiple
POLR2D
decreased
proliferation.
related
transcription
just
subset
POL
transcribe
genes,
indicating
distinct
role.
Taken
together,
have
shown
regulation
genes
common
tumors.
demonstrated
cancer-specific
function
genes.
