Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations DOI Creative Commons

Vanessa Rodwell,

Manjiri Patil,

Helen J. Kuht

et al.

Biology, Journal Year: 2023, Volume and Issue: 13(1), P. 4 - 4

Published: Dec. 20, 2023

Optokinetic reflex (OKR) assays in zebrafish models are a valuable tool for studying diverse range of ophthalmological and neurological conditions. Despite its increasing popularity recent years, there no clear reporting guidelines the assay. Following research enhances reproducibility, reduces bias, mitigates underreporting poor methodologies published works. To better understand optimal standards an OKR assay zebrafish, we performed systematic literature review exploring animal, environmental, technical factors that should be considered. Using search criteria from three online databases, total 109 papers were selected review. Multiple crucial identified, including larval characteristics, sample size, fixing method, set-up, distance stimulus, detailed stimulus parameters, eye recording, movement analysis. The outcome analysis highlighted insufficient information provided past lack way to present parameters related each experimental factors. circumvent any future errors champion robust transparent research, have created optokinetic (ZOK) minimal guideline.

Language: Английский

Transcriptional patterns of human retinal pigment epithelial cells under protracted high glucose DOI
Hao Huang,

Jingshu Zeng,

Xielan Kuang

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: April 4, 2024

Language: Английский

Citations

1
Investigating G-quadruplex structures in RPGR gene: Implications for understanding X-linked retinal degeneration DOI Creative Commons
Luigi Donato, Concetta Scimone, Simona Alibrandi

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(8), P. e29828 - e29828

Published: April 1, 2024

This pilot study investigates the potential pathogenic role of G-quadruplex (G4) structures in

Language: Английский

Citations

1
FDXR-associated disease in a Chinese cohort: Unraveling expanded ocular phenotypes and genetic spectrum DOI
Xing Wei, Hui Li,

Tian Zhu

et al.

Experimental Eye Research, Journal Year: 2023, Volume and Issue: 234, P. 109600 - 109600

Published: July 20, 2023

Language: Английский

Citations

3
Pan-cancer integrated bioinformatic analysis of RNA polymerase subunits reveal RNA Pol I member CD3EAP regulates cell growth by modulating autophagy DOI

Nikita Bhandari,

Disha Acharya, Annesha Chatterjee

et al.

Cell Cycle, Journal Year: 2023, Volume and Issue: 22(18), P. 1986 - 2002

Published: Sept. 17, 2023

Transcription is a crucial stage in gene expression. An integrated study of 34 RNA polymerase subunits (RNAPS) the six most frequent cancer types identified several genetic and epigenetic modification. We discovered nine mutant RNAPS with mutation frequency more than 1% at least one tumor type. POLR2K POLR2H were found to be amplified overexpressed, whereas POLR3D was deleted downregulated. Multiple also observed regulated by variations promoter methylation. 5-Aza-2-deoxycytidine mediated re-expression cell lines verified methylation-driven inhibition POLR2F POLR2L expression BRCA NSCLC, respectively. Next, we showed that CD3EAP, Pol I subunit, overexpressed all associated worst survival breast, liver, lung, prostate cancers. The knockdown studies CD3EAP required for proliferation induces autophagy but not apoptosis. Furthermore, rescued cells. correlated S G2 phase cycle regulators, inhibited CDK/cyclins. POLR2D, an pol II as commonly prognostic multiple POLR2D decreased proliferation. related transcription just subset POL transcribe genes, indicating distinct role. Taken together, have shown regulation genes common tumors. demonstrated cancer-specific function genes.

Language: Английский

Citations

3
Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations DOI Creative Commons

Vanessa Rodwell,

Manjiri Patil,

Helen J. Kuht

et al.

Biology, Journal Year: 2023, Volume and Issue: 13(1), P. 4 - 4

Published: Dec. 20, 2023

Optokinetic reflex (OKR) assays in zebrafish models are a valuable tool for studying diverse range of ophthalmological and neurological conditions. Despite its increasing popularity recent years, there no clear reporting guidelines the assay. Following research enhances reproducibility, reduces bias, mitigates underreporting poor methodologies published works. To better understand optimal standards an OKR assay zebrafish, we performed systematic literature review exploring animal, environmental, technical factors that should be considered. Using search criteria from three online databases, total 109 papers were selected review. Multiple crucial identified, including larval characteristics, sample size, fixing method, set-up, distance stimulus, detailed stimulus parameters, eye recording, movement analysis. The outcome analysis highlighted insufficient information provided past lack way to present parameters related each experimental factors. circumvent any future errors champion robust transparent research, have created optokinetic (ZOK) minimal guideline.

Language: Английский

Citations

3