Unraveling antiviral efficacy of multifunctional immunomodulatory triterpenoids against SARS-COV-2 targeting main protease and papain-like protease DOI Creative Commons

Shweta Choudhary,

Sanketkumar Nehul, Ankur Singh

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 26, 2023

Abstract The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2) may be over, but its variants continue to emerge, and patients with mild symptoms having long COVID is still under investigation. SARS-CoV-2 infection leading elevated cytokine levels suppressed immune responses set off storm, fatal systemic inflammation, tissue damage, multi-organ failure. Thus, drug molecules targeting the virus-specific proteins or capable of suppressing host inflammatory viral would provide an effective antiviral therapy against emerging concern. Evolutionarily conserved papain-like protease (PLpro) main (Mpro) play indispensable role in virus life cycle evasion. Direct-acting antivirals both these proteases represent attractive strategy that also expected reduce inflammation. present study has evaluated anti-inflammatory potential natural triterpenoids: azadirachtin, withanolide_A, isoginkgetin. These inhibit Mpro PLpro proteolytic activities half-maximal inhibitory concentrations (IC 50 ) values ranging from 1.42 32.7 µM. Isothermal titration calorimetry (ITC) analysis validated binding compounds PLpro. As expected, two compounds, withanolide_A exhibit potent anti-SARS-CoV-2 activity cell-based assays, half- maximum concentration (EC 21.73 µM 31.19 µM, respectively. azadirachtin when assessed using HEK293T cells were found significantly CXCL10, TNFα, IL6, IL8 cytokines, which are cases COVID-19. Interestingly, rescue decreased type-I interferon response (IFN-α1). results this clearly highlight triterpenoids as target specific enzymes pathways involved mediated

Язык: Английский

An Overview of Biofilm-Associated Infections and the Role of Phytochemicals and Nanomaterials in Their Control and Prevention DOI Creative Commons

Tsvetozara Damyanova,

Petya D. Dimitrova,

Dayana Borisova

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(2), С. 162 - 162

Опубликована: Янв. 24, 2024

Biofilm formation is considered one of the primary virulence mechanisms in Gram-positive and Gram-negative pathogenic species, particularly those responsible for chronic infections promoting bacterial survival within host. In recent years, there has been a growing interest discovering new compounds capable inhibiting biofilm formation. This promising antivirulence strategy that could potentially overcome antibiotic resistance issues. Effective antibiofilm agents should possess distinctive properties. They be structurally unique, enable easy entry into cells, influence quorum sensing signaling, synergize with other antibacterial agents. Many these properties are found both natural systems isolated from plants synthetic like nanoparticles nanocomposites. this review, we discuss clinical nature biofilm-associated some associated their tolerance. We focus on advantages efficacy various as therapeutic approach to control biofilms address multidrug bacteria.

Язык: Английский

Процитировано

16
Green and efficient extraction of phenolic compounds from Neem leaves using deep eutectic solvents based ultrasonic-assisted extraction DOI
Kirandeep Κaur, Philippe Schmitt‐Kopplin, Ashok Kumar Malik

и другие.

Food Chemistry, Год журнала: 2024, Номер 451, С. 139500 - 139500

Опубликована: Апрель 27, 2024

Язык: Английский

Процитировано

8
Chromobacterium Violaceum: A Model for Evaluating the Anti-Quorum Sensing Activities of Plant Substances DOI Creative Commons

Petya D. Dimitrova,

Tsvetozara Damyanova,

Tsvetelina Paunova‐Krasteva

и другие.

Scientia Pharmaceutica, Год журнала: 2023, Номер 91(3), С. 33 - 33

Опубликована: Июль 3, 2023

In the new antibiotic era, exponential increase in multiresistant bacterial strains has become main global health problem. Many researchers have focused their efforts on exploring novel or combined strategies for combating resistance. Good knowledge of molecular mechanisms resistance and virulence factors as key targets provides us with a good basis resolving One particularly attractive promising strategy is to attack regulatory “network” determinants known quorum sensing (QS). The inhibition QS signals will be means screening more effective quorum-sensing inhibitors (QSIs) play role use next-generation antimicrobials battle against This motivated present review provide comprehensive clarification signaling pathways Chromobacterium violaceum discovery potential plant inhibitors.

Язык: Английский

Процитировано

20
Unraveling antiviral efficacy of multifunctional immunomodulatory triterpenoids against SARS‐COV‐2 targeting main protease and papain‐like protease DOI Open Access

Shweta Choudhary,

Sanketkumar Nehul, Ankur Singh

и другие.

IUBMB Life, Год журнала: 2023, Номер 76(5), С. 228 - 241

Опубликована: Дек. 7, 2023

Abstract The coronavirus disease 2019 (COVID‐19) pandemic, caused by severe acute respiratory syndrome 2 (SARS‐CoV‐2) may be over, but its variants continue to emerge, and patients with mild symptoms having long COVID is still under investigation. SARS‐CoV‐2 infection leading elevated cytokine levels suppressed immune responses set off storm, fatal systemic inflammation, tissue damage, multi‐organ failure. Thus, drug molecules targeting the virus‐specific proteins or capable of suppressing host inflammatory viral would provide an effective antiviral therapy against emerging concern. Evolutionarily conserved papain‐like protease (PLpro) main (Mpro) play indispensable role in virus life cycle evasion. Direct‐acting antivirals both these proteases represent attractive strategy that also expected reduce inflammation. present study has evaluated anti‐inflammatory potential natural triterpenoids: azadirachtin, withanolide_A, isoginkgetin. These inhibit Mpro PLpro proteolytic activities half‐maximal inhibitory concentrations (IC 50 ) values ranging from 1.42 32.7 μM. Isothermal titration calorimetry (ITC) analysis validated binding compounds PLpro. As expected, two compounds, withanolide_A exhibit potent anti‐SARS‐CoV‐2 activity cell‐based assays, half‐maximum concentration (EC 21.73 31.19 μM, respectively. roles azadirachtin when assessed using HEK293T cells, were found significantly CXCL10, TNFα, IL6, IL8 cytokines, which are cases COVID‐19. Interestingly, rescue decreased type‐I interferon response (IFN‐α1). results this clearly highlight triterpenoids as target SARS‐CoV‐2‐specific enzymes pathways involved virus‐mediated

Язык: Английский

Процитировано

9
Bactericidal action of cost-effective colorimetric acid-base sensor of dyed cotton-based fabrics as a promising wound dressing mat DOI
Mervat El‐Sedik,

Bahaa A. Hemdan,

Mohamed Hashem

и другие.

Journal of Photochemistry and Photobiology A Chemistry, Год журнала: 2024, Номер 456, С. 115837 - 115837

Опубликована: Июнь 15, 2024

Язык: Английский

Процитировано

3
Unveiling the antiviral potential of Plant compounds from the Meliaceae family against the Zika virus through QSAR modeling and MD simulation analysis DOI
Divyanshi Shukla, Amer M. Alanazi, Siva Prasad Panda

и другие.

Journal of Biomolecular Structure and Dynamics, Год журнала: 2023, Номер 42(20), С. 11064 - 11079

Опубликована: Сен. 20, 2023

Zika virus (ZIKV) is a flavivirus transmitted by mosquitoes, causing neurological disorders and congenital malformations. RNA-dependent RNA polymerase (RdRp) one of its essential enzymes promising drug target for antiviral therapy due to involvement in the growth multiplication virus. In this study, we conducted QSAR-based chemical library screening from Meliaceae family identify potential RdRp inhibitors. The QSAR model was built using known inhibitors NS5 ZIKV their biological activity (EC50), along with structural characteristics compounds. top two hit compounds were selected further analysis molecular docking evaluate binding energies intermolecular interactions RdRp, including critical residue Trp

Язык: Английский

Процитировано

8
Synthesis and application of a new multi-functional biopolymer-based aerogel loaded with bistriazole derivative as highly efficient adsorbent and disinfectant DOI
Emad K. Radwan,

Bahaa A. Hemdan,

Shaimaa T. El‐Wakeel

и другие.

Journal of Cleaner Production, Год журнала: 2023, Номер 434, С. 139932 - 139932

Опубликована: Дек. 2, 2023

Язык: Английский

Процитировано

7
Swieteliacates S–U, phragmalin limonoids, from the leaves of Swietenia macrophylla DOI

Yunpeng Sun,

Zhe Xie,

Wenfang Jin

и другие.

Organic & Biomolecular Chemistry, Год журнала: 2024, Номер 22(11), С. 2182 - 2186

Опубликована: Янв. 1, 2024

Three novel phragmalin-class limonoids (1–3) along with four known analogues were isolated and identified from the leaves of S. macrophylla .

Язык: Английский

Процитировано

2
Antibacterial Properties of Methanolic Leaf Extracts of Melia azedarach L. against Gram-Positive and Gram-Negative Pathogenic Bacteria DOI Creative Commons
Soraya Naila Touzout, Abderrahmen Merghni, Aïcha Laouani

и другие.

Microorganisms, Год журнала: 2023, Номер 11(8), С. 2062 - 2062

Опубликована: Авг. 11, 2023

Melia azedarach L., a Meliaceae family tree, is widely used in traditional folkloric medicine for its pharmaceutical properties. In the present study, we investigated phytochemical composition of four methanolic leaf extracts M. various origins (Algeria and Tunisia) using high-performance liquid chromatography (HPLC). The antibacterial efficacy mechanisms action against Gram-positive Gram-negative pathogenic microorganisms were then evaluated. Our findings revealed presence phenolic acids flavonoids, such as gallic acid, chlorogenic caffeic hyperoside, isoquercetin, quercetin, isorhamnetin both Algerian Tunisian localities, with an abundance compared to flavonoids. Additionally, studied exhibit broad spectrum activities, MIC values ranging from 31.25 mg/mL 125 mg/mL. Methanolic Algeria exhibited more potent biofilm eradication, percentage inhibition reaching 72.17% S. aureus strain. Furthermore, inhibitory concentrations tested substances, particularly extract Relizane area, capable disrupting membrane integrity treated bacteria well producing oxidative stress through ROS generation. Likewise, our results reveal that plant induces lipid peroxidation by raising MDA levels comparison untreated cells, Blida. also reduced synthesis antioxidant enzymes (CAT SOD). illustrate remains significant potential distinct are closely related this specimen.

Язык: Английский

Процитировано

5
Natural Antimicrobials: A Reservoir to Contrast Listeria monocytogenes DOI Creative Commons
Annalisa Ricci, Camilla Lazzi, Valentina Bernini

и другие.

Microorganisms, Год журнала: 2023, Номер 11(10), С. 2568 - 2568

Опубликована: Окт. 15, 2023

Natural environments possess a reservoir of compounds exerting antimicrobial activity that are forms defence for some organisms against others. Recently, they have become more and attractive in the food sector due to increasing demand natural capacity protect from pathogenic microorganisms. Among foodborne pathogens, Listeria monocytogenes can contaminate during production, distribution, or storage, its presence is especially detected fresh, raw ready-to-eat products. The interest this microorganism related listeriosis, severe disease with high mortality rate occur after ingestion. Starting premise, present review aims investigate plant extract fermented matrices, as well mixtures produced microbial fermentation processes anti-listeria activity.

Язык: Английский

Процитировано

5