Integrated Analysis of Transcriptomics and Proteomics Provides Insights into the Accumulation Mechanism of Ascorbic Acid in Rosa roxburghii Tratt
Foods,
Год журнала:
2025,
Номер
14(5), С. 748 - 748
Опубликована: Фев. 22, 2025
Rosa
roxburghii
Tratt
(RRT)
is
widely
cultivated
in
Guizhou
Province,
China.
In
recent
years,
RRT
has
emerged
as
one
of
the
most
promising
new
fruit
crops
China,
primarily
because
its
remarkably
high
levels
ascorbic
acid
(AsA).
this
research,
we
assessed
AsA
across
various
growth
phases.
The
findings
demonstrate
that
concentration
fruits
progressively
increased
a
linear
fashion
throughout
development,
peaking
at
2274.60
mg/(100
g
FW)
when
reached
maturity
(84
DAA).
Furthermore,
conducted
an
integrated
analysis
transcriptomic
and
proteomic
data
for
first
time
to
investigate
mechanisms
responsible
accumulation
RRT.
Our
results
show
differentially
expressed
genes
(DEGs)
abundant
proteins
(DAPs)
were
associated
with
ascorbate
aldarate
metabolism
pathway,
suggesting
pathway
plays
crucial
role
regulating
This
study
elucidates
molecular
mechanism
underlying
provides
robust
scientific
foundation
subsequent
research
on
Язык: Английский
Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis
AMB Express,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 5, 2025
Acute
pancreatitis
(AP)
is
a
prevalent
acute
gastrointestinal
disease,
which
may
be
prevented
and
alleviated
by
probiotics.
Bifidobacterium
animalis
subsp.
lactis
BB-12
(BB-12)
widely
studied
probiotic
strain;
however,
its
specific
effects
in
this
context
remain
unexplored.
In
study,
we
aimed
to
investigate
the
prophylactic
therapeutic
of
AP.
Our
findings
revealed
that
administration
via
gavage
significantly
reduced
pathological
pancreatic
damage
serum
amylase
activity.
Microbiome
analysis
showed
treatment
increased
relative
abundance
Ligilactobacillus
decreased
Bilophila
gut
microbiota
mice
with
Transcriptome
mitigated
AP-induced
dysregulation
several
pathways,
specifically
attenuating
upregulation
secretion
ascorbate
aldarate
metabolism
pathways
while
reversing
downregulation
ribosome,
oxidative
phosphorylation,
thermogenesis
pathways.
Spearman's
correlation
positive
between
abundances
ASF356
Furthermore,
were
correlated
BB-12-regulated
genes
predominantly
enriched
ribosome
pathway.
conclusion,
pretreatment
AP,
likely
regulating
intestinal
Lactobacillus,
Bilophila,
ASF356,
as
well
secretion,
metabolism,
Язык: Английский
The gut–kidney axis is regulated by astragaloside IV to inhibit cyclosporine A-induced nephrotoxicity
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 27, 2025
Introduction
Chronic
nephrotoxicity
caused
by
CNIs
(CICN)
manifests
clinically
as
chronic
kidney
disease
(CKD).
Astragaloside
IV
(AS-IV)
plays
a
certain
role
in
the
treatment
of
CKD.
This
study
aimed
to
verify
ameliorative
effects
AS-IV
on
CICN
and
further
explore
mechanisms
underlying
modulation
“gut–transcriptome–metabolome
coexpression
network”
within
context
“gut–kidney
axis”
improve
CICN.
Methods
Five
groups
40
mice
were
studied:
normal
group
(N,
olive
oil),
model
(M,
CsA,
30
mg
kg
-−1
d
−1
),
low-dose
(CsA
+
AS-IV,
10
high-dose
20
valsartan
Val,
).
The
gut
microbiota,
renal
transcriptome,
urine
metabolome
separately
detected
construct
gut–transcriptome–metabolome
network.
target
species,
genes,
metabolites
evaluated.
Results
CsA
led
increased
proteinuria
deterioration
function,
accompanied
inflammation
oxidative
stress,
whereas
improved
damage.
inhibited
intestinal
permeability
disrupted
microbiota
structure,
increasing
abundance
Lactobacillus
reuteri
,
Bifidobacterium
animalis
Ignatzschineria
indica
Blautia
glucerasea.
Six
pathways
related
transcription
metabolism,
including
citrate
cycle
ascorbate
aldarate
metabolism
proximal
tubule
bicarbonate
reclamation
glycolysis/gluconeogenesis,
ferroptosis,
drug
metabolism–cytochrome
P450
identified.
Seven
identified
6
pathways,
UDP-D-galacturonic
acid,
2-phenylethanol
glucuronide,
dehydroascorbic
isopentenyl
pyrophosphate,
alpha-D-glucose,
3-carboxy-1-hydroxypropylthiamine
diphosphate
citalopram
aldehyde.
genes
Ugt1a2,
Ugt1a9,
Ugt1a5,
Pck1,
Slc7a11,
also
predicted
NONMMUT144584.1,
MSTRG.30357.1
ENSMUST00000174821.
was
highly
correlated
with
function
AS-IV.
validated.
intestinal-derived
urinary
toxins
tissue
apoptosis,
lipid
accumulation,
collagen
deposition,
mitochondrial
Conclusion
through
six
energy
driven
L.
alderate
tube
are
important
mechanisms.
Язык: Английский
Serum metabolic profiling of patients with diabetic kidney disease based on gas chromatography-mass spectrometry
Frontiers in Molecular Biosciences,
Год журнала:
2025,
Номер
12
Опубликована: Март 17, 2025
Given
the
increasing
incidence
rate
of
diabetic
kidney
disease
(DKD),
there
is
an
urgent
need
for
methods
to
diagnose
and
treat
DKD
in
clinics.
Serum
samples
were
collected
from
56
patients
32
healthy
controls
(HCs)
at
First
Affiliated
Hospital
Ningbo
University,
metabolic
profiles
obtained
through
untargeted
metabolomics
using
gas
chromatography
mass
spectrometry.
The
data
then
analyzed
principal
components
analysis,
orthogonal
partial
least-squares
discriminant
Pearson
correlation
receiver
operating
characteristic
(ROC)
curve.
It
was
found
that
serum
significantly
different
those
HCs.
A
total
68
potential
differential
metabolites
identified
involved
arginine
biosynthesis,
ascorbate
aldarate
metabolism,
galactose
among
others;
a
31
also
between
early-stage
(EDG)
late-stage
(LDG)
patients.
Additionally,
30
significant
differences
observed
EDG,
LDG,
HC
groups.
Based
on
analysis
abundances
clinical
markers
(estimated
glomerular
filtration
rate,
blood
urea
nitrogen,
creatinine,
urinary
albumin/creatinine
ratio)
area
under
ROC
curve
(AUROC)
AUROC
values
myoinositol
gluconic
acid
be
0.992
0.991,
respectively,
which
can
used
distinguish
These
results
indicate
could
possibly
as
biomarkers
DKD.
Язык: Английский
Dendrobium officinale leaf phenolic extracts alleviate diabetes mellitus in mice via modulating metabolism and reshaping gut microbiota
Journal of the Science of Food and Agriculture,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 29, 2025
Abstract
BACKGROUND
Diabetes
mellitus
is
a
chronic
metabolic
disorder
posing
significant
public
health
challenge.
Dendrobium
officinale
valuable
edible‐medicinal
homologous
plant.
Phenolic
extracts
from
fermented
D.
leaves
(DOLP)
demonstrate
hypoglycemic
effect.
However,
the
effects
of
DOLP
on
physiological
metabolism
and
gut
microbiota
under
diabetic
conditions
remain
unknown.
RESULTS
Untargeted
metabolomics
analysis
revealed
that
reversed
98
significantly
differential
metabolites
(SDMs)
in
mice.
Indoxyl
sulfate
palmitoyl
sphingomyelin
were
identified
as
primary
candidate
biomarkers
associated
with
KEGG
pathway
enrichment
underscored
significance
arginine
proline
metabolism,
ascorbate
aldarate
fatty
acid
regulatory
DOLP.
Furthermore,
dysbiosis
mice,
influencing
relative
abundance
genera
Intestinimonas
,
GCA_900066575
Muribaculum
Enterorhabdus
.
These
exhibited
various
correlations
SDMs,
including
l
‐hydroxyarginine,
γ‐guanidinobutyrate,
‐threonate,
d
‐galactarate,
‐ascorbic
acid,
palmitic
cis
‐9‐palmitoleic
octadecanoic
oleic
acid.
CONCLUSION
The
protective
effect
against
diabetes
closely
linked
to
its
regulation
microbiota.
may
serve
promising
agent
for
prevention
treatment
mellitus.
©
2025
Society
Chemical
Industry.
Язык: Английский
Untargeted Metabolite Profiling Reveals Acute Toxicity of Pentosidine on Adipose Tissue of Rats
Metabolites,
Год журнала:
2024,
Номер
14(10), С. 539 - 539
Опубликована: Окт. 9, 2024
:
Pentosidine
is
an
advanced
glycation
end
product
that
commonly
found
in
heat-processed
foods.
has
been
involved
the
occurrence
and
development
of
some
chronic
diseases.
It
was
reported
pentosidine
exposure
can
impair
function
liver
kidneys.
Adipose
tissue,
as
active
endocrine
organ,
plays
important
role
maintaining
normal
physiological
cells.
However,
metabolic
mechanism
causes
to
induce
toxicity
adipose
tissue
remains
unclear.
Язык: Английский