Integrated Analysis of Transcriptomics and Proteomics Provides Insights into the Accumulation Mechanism of Ascorbic Acid in Rosa roxburghii Tratt

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 748 - 748

Published: Feb. 22, 2025

Rosa roxburghii Tratt (RRT) is widely cultivated in Guizhou Province, China. In recent years, RRT has emerged as one of the most promising new fruit crops China, primarily because its remarkably high levels ascorbic acid (AsA). this research, we assessed AsA across various growth phases. The findings demonstrate that concentration fruits progressively increased a linear fashion throughout development, peaking at 2274.60 mg/(100 g FW) when reached maturity (84 DAA). Furthermore, conducted an integrated analysis transcriptomic and proteomic data for first time to investigate mechanisms responsible accumulation RRT. Our results show differentially expressed genes (DEGs) abundant proteins (DAPs) were associated with ascorbate aldarate metabolism pathway, suggesting pathway plays crucial role regulating This study elucidates molecular mechanism underlying provides robust scientific foundation subsequent research on

Language: Английский

---

Role of Bifidobacterium animalis subsp. lactis BB-12 in mice with acute pancreatitis

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 5, 2025

Acute pancreatitis (AP) is a prevalent acute gastrointestinal disease, which may be prevented and alleviated by probiotics. Bifidobacterium animalis subsp. lactis BB-12 (BB-12) widely studied probiotic strain; however, its specific effects in this context remain unexplored. In study, we aimed to investigate the prophylactic therapeutic of AP. Our findings revealed that administration via gavage significantly reduced pathological pancreatic damage serum amylase activity. Microbiome analysis showed treatment increased relative abundance Ligilactobacillus decreased Bilophila gut microbiota mice with Transcriptome mitigated AP-induced dysregulation several pathways, specifically attenuating upregulation secretion ascorbate aldarate metabolism pathways while reversing downregulation ribosome, oxidative phosphorylation, thermogenesis pathways. Spearman's correlation positive between abundances ASF356 Furthermore, were correlated BB-12-regulated genes predominantly enriched ribosome pathway. conclusion, pretreatment AP, likely regulating intestinal Lactobacillus, Bilophila, ASF356, as well secretion, metabolism,

Language: Английский

---

The gut–kidney axis is regulated by astragaloside IV to inhibit cyclosporine A-induced nephrotoxicity

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 27, 2025

Introduction Chronic nephrotoxicity caused by CNIs (CICN) manifests clinically as chronic kidney disease (CKD). Astragaloside IV (AS-IV) plays a certain role in the treatment of CKD. This study aimed to verify ameliorative effects AS-IV on CICN and further explore mechanisms underlying modulation “gut–transcriptome–metabolome coexpression network” within context “gut–kidney axis” improve CICN. Methods Five groups 40 mice were studied: normal group (N, olive oil), model (M, CsA, 30 mg kg -−1 d −1 ), low-dose (CsA + AS-IV, 10 high-dose 20 valsartan Val, ). The gut microbiota, renal transcriptome, urine metabolome separately detected construct gut–transcriptome–metabolome network. target species, genes, metabolites evaluated. Results CsA led increased proteinuria deterioration function, accompanied inflammation oxidative stress, whereas improved damage. inhibited intestinal permeability disrupted microbiota structure, increasing abundance Lactobacillus reuteri , Bifidobacterium animalis Ignatzschineria indica Blautia glucerasea. Six pathways related transcription metabolism, including citrate cycle ascorbate aldarate metabolism proximal tubule bicarbonate reclamation glycolysis/gluconeogenesis, ferroptosis, drug metabolism–cytochrome P450 identified. Seven identified 6 pathways, UDP-D-galacturonic acid, 2-phenylethanol glucuronide, dehydroascorbic isopentenyl pyrophosphate, alpha-D-glucose, 3-carboxy-1-hydroxypropylthiamine diphosphate citalopram aldehyde. genes Ugt1a2, Ugt1a9, Ugt1a5, Pck1, Slc7a11, also predicted NONMMUT144584.1, MSTRG.30357.1 ENSMUST00000174821. was highly correlated with function AS-IV. validated. intestinal-derived urinary toxins tissue apoptosis, lipid accumulation, collagen deposition, mitochondrial Conclusion through six energy driven L. alderate tube are important mechanisms.

Language: Английский

---

Serum metabolic profiling of patients with diabetic kidney disease based on gas chromatography-mass spectrometry

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 12

Published: March 17, 2025

Given the increasing incidence rate of diabetic kidney disease (DKD), there is an urgent need for methods to diagnose and treat DKD in clinics. Serum samples were collected from 56 patients 32 healthy controls (HCs) at First Affiliated Hospital Ningbo University, metabolic profiles obtained through untargeted metabolomics using gas chromatography mass spectrometry. The data then analyzed principal components analysis, orthogonal partial least-squares discriminant Pearson correlation receiver operating characteristic (ROC) curve. It was found that serum significantly different those HCs. A total 68 potential differential metabolites identified involved arginine biosynthesis, ascorbate aldarate metabolism, galactose among others; a 31 also between early-stage (EDG) late-stage (LDG) patients. Additionally, 30 significant differences observed EDG, LDG, HC groups. Based on analysis abundances clinical markers (estimated glomerular filtration rate, blood urea nitrogen, creatinine, urinary albumin/creatinine ratio) area under ROC curve (AUROC) AUROC values myoinositol gluconic acid be 0.992 0.991, respectively, which can used distinguish These results indicate could possibly as biomarkers DKD.

Language: Английский

---

Dendrobium officinale leaf phenolic extracts alleviate diabetes mellitus in mice via modulating metabolism and reshaping gut microbiota

Journal of the Science of Food and Agriculture, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

Abstract BACKGROUND Diabetes mellitus is a chronic metabolic disorder posing significant public health challenge. Dendrobium officinale valuable edible‐medicinal homologous plant. Phenolic extracts from fermented D. leaves (DOLP) demonstrate hypoglycemic effect. However, the effects of DOLP on physiological metabolism and gut microbiota under diabetic conditions remain unknown. RESULTS Untargeted metabolomics analysis revealed that reversed 98 significantly differential metabolites (SDMs) in mice. Indoxyl sulfate palmitoyl sphingomyelin were identified as primary candidate biomarkers associated with KEGG pathway enrichment underscored significance arginine proline metabolism, ascorbate aldarate fatty acid regulatory DOLP. Furthermore, dysbiosis mice, influencing relative abundance genera Intestinimonas , GCA_900066575 Muribaculum Enterorhabdus . These exhibited various correlations SDMs, including l ‐hydroxyarginine, γ‐guanidinobutyrate, ‐threonate, d ‐galactarate, ‐ascorbic acid, palmitic cis ‐9‐palmitoleic octadecanoic oleic acid. CONCLUSION The protective effect against diabetes closely linked to its regulation microbiota. may serve promising agent for prevention treatment mellitus. © 2025 Society Chemical Industry.

Language: Английский

---

Untargeted Metabolite Profiling Reveals Acute Toxicity of Pentosidine on Adipose Tissue of Rats

Metabolites, Journal Year: 2024, Volume and Issue: 14(10), P. 539 - 539

Published: Oct. 9, 2024

: Pentosidine is an advanced glycation end product that commonly found in heat-processed foods. has been involved the occurrence and development of some chronic diseases. It was reported pentosidine exposure can impair function liver kidneys. Adipose tissue, as active endocrine organ, plays important role maintaining normal physiological cells. However, metabolic mechanism causes to induce toxicity adipose tissue remains unclear.

Language: Английский

---