Comprehensive analysis of vaginal microbiota in Chinese women with genital tuberculosis: implications for diagnosis and treatment

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 28, 2025

Tuberculosis remains an infectious disease of global concern, with potential impacts on respiratory and intestinal microbiota owing to prolonged broad-spectrum antibiotic therapy. Despite its cause infertility, the vaginal women genital tuberculosis poorly understood. We comprehensively analyzed in Chinese tuberculosis. recruited pelvic (n = 28), endometrial 16), pulmonary 12) as research group, healthy 11) control group. Vaginal discharges were collected for metagenomic analysis microbiota. The alpha diversity was slightly higher than that women, though difference not statistically significant (P 0.23). Similarly, no differences observed between 0.82) or those 0.82). Notably, lowest recorded six months one year after initiating anti-tuberculosis treatment, this decline being 0.023). dominance Lactobacillus iners more common crispatus. Furthermore, abundance short-chain fatty acid -producing anaerobes, such Actinomycetes, Streptococcus, Finegoldia, significantly increased. Short-chain precursor pathways, including ko03010 ribosome pathway, ko00970 aminoacyl-tRNA synthesis, ko00230 purine metabolism, ko00240 pyrimidine ko00010 glycolysis gluconeogenesis upregulated Extrapulmonary tuberculosis, particularly associated dysbiosis female fecundity. is pronounced when M. invades endometrium. Given effect antibiotics flora, probiotic combined interventions could be used a future direction. Not applicable.

Язык: Английский

---

Peripheral blood microbiome signature and Mycobacterium tuberculosis-derived rsRNA as diagnostic biomarkers for tuberculosis in human

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 19, 2025

Tuberculosis (TB) is a major global health issue. Early diagnosis of TB still challenge. Studies are seeking non-sputum biomarker-based test. Emerging evidence indicates potential significance blood microbiome signatures for diseases. However, RNA profiles unknown in TB. We aimed to characterize the patients and identify Mycobacterium tuberculosis (Mtb) genome-derived small molecules serve as diagnostic biomarkers sequencing data from healthy controls were retrieved NCBI-SRA database analyzing identifying rRNA-derived (rsRNA) Mtb. Small RNA-seq was performed on plasma exosomes controls. The levels candidate Mtb rsRNAs determined by real-time quantitative reverse transcription PCR (RT-qPCR) separate cohort 73 62 consisted signals bacteria, fungi, archaea, viruses, with bacteria accounting more than 97% total. Reduced microbial diversity abundance 6 Mycobacterium-associated bacterial genera, including Mycobacterium, Priestia, Nocardioides, Agrobacterium, Bradyrhizobium, Escherichia, significantly altered patients. A model based genera achieved an area under curve (AUC) 0.8945. mapped genome identified RT-qPCR results showed that 2 Mtb-derived rsRNAs, 16 S-L1 S-L2, could be used differentiate controls, high co-diagnostic efficacy (AUC = 0.7197). panel can diagnosis.

Язык: Английский

---

The Microbiome in Asthma Heterogeneity: The Role of Multi‐Omic Investigations

Immunological Reviews, Год журнала: 2025, Номер 330(1)

Опубликована: Март 1, 2025

ABSTRACT Asthma is one of the most prevalent and extensively studied chronic respiratory conditions, yet heterogeneity asthma remains biologically puzzling. Established factors like exogenous exposures treatment adherence contribute to variability in risk clinical outcomes. It also clear that endogenous genetics immune system response patterns play key roles asthma. Despite significant existing knowledge above, divergent trajectories outcomes are still observed, even among individuals with similar profiles, biomarkers, optimal medical management. This suggests uncaptured biological interactions asthma's heterogeneity, for which role host microbiota has lately attracted much research attention. review will highlight recent evidence this area, focusing on bedside‐to‐bench investigations have leveraged omic technologies uncover microbiome links immunobiology. Studies centered use multi‐omics noted particular. These represent a new generation reverse‐translational revealing potential functional crosstalk microbiomes may drive phenotypic diseases Multi‐omic data offer wide lens into ecosystem within host. informs hypotheses experimental work elucidate mechanistic pathways unresolved endotypes. Further incorporation patient‐centered can yield insights hopefully lead more precise, microbiome‐informed strategies reduce burden.

Язык: Английский

---

The clinical value of metagenomic next-generation sequencing in the diagnosis of pulmonary tuberculosis and the exploration of lung microbiota characteristics

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 12, 2025

The lung microbiota plays a critical role in many important physiological processes and is linked with various pulmonary infectious diseases. present study aimed to characterize the patients tuberculosis (PTB), explore association between abundance of Mycobacterium complex (MTBC) microbiota. This retrospective included 190 MTBC infection. enrolled were classified into three groups based on results bronchoalveolar lavage fluid (BALF) mNGS: low [reads per ten million (RPTM) = 1 ~ 1000], medium (RPTM 1001 10000) high > 10000). In high-abundance group, there more bilateral lobar involvement symptoms cavitation. addition mNGS, highest positivity rates T-spot (92.36%), GeneXpert (71.58%), culture (68.95%) AFB smear (17.84%). positive culture, increased increase abundance, group. Both alpha beta diversity showed significant difference groups, lowest diversity. positively associated longer time hospital stay. To sum up, PTB significantly distinct different abundant levels MTBC. Combined imaging features, suggests patient severely ill has poorer prognosis.

Язык: Английский

---

Role of the gut lung axis and microbiota based approaches in the treatment of tuberculosis

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 159 - 175

Опубликована: Янв. 1, 2025

Язык: Английский

---

Airway microbiome signature accurately discriminates Mycobacterium tuberculosis infection status

iScience, Год журнала: 2024, Номер 27(6), С. 110142 - 110142

Опубликована: Май 28, 2024

remains one of the deadliest infectious agents globally. Amidst efforts to control TB, long treatment duration, drug toxicity, and resistance underscore need for novel therapeutic strategies. Despite advances in understanding interplay between microbiome disease humans, specific role predicting susceptibility discriminating infection status tuberculosis still needs be fully investigated. We investigated impact

Язык: Английский

---

A Robust In Vitro Anti‐tuberculosis, Antimicrobial, and Anti‐inflammatory Activities Based on Azomethine Chelates Incorporating Co(II), Ni (II), Cu(II), and Zn(II) Ions: Synthesis, Characterization, and Investigation of the Aspects of Docking Interaction

Applied Organometallic Chemistry, Год журнала: 2024, Номер 38(11)

Опубликована: Авг. 12, 2024

ABSTRACT In recent times, there has been a growing exploration of transition metal complexes as potential solutions for significant health challenges, including tuberculosis, microbes infection, and inflammation. Therefore, in our ongoing effort to identify biologically effective agents, Co(II), Ni(II), Cu(II), Zn(II) H 2 L 1 –H hydrazone ligands were synthesized. The structural features synthesized compounds recognized by employing several techniques such FT‐IR, NMR, 13 C powder x‐ray diffraction (XRD), UV‐Vis, ESR, TG‐DTA, mass spectrometry, molar conductance measurements. bonding via O phenolic , enolic N azomethine donor atoms the attachment three water molecules with ion form octahedral structure corroborated different spectroscopic techniques. anti‐tuberculosis, antimicrobial, anti‐inflammatory activities assessed using microplate alamar blue assay, serial dilution, bovine serum albumin (BSA) methods, respectively, highlighted more potency than ligands. Cu(II) (9 ) ( 10 exhibited excellent ability inhibit growth 37 R v strain Mycobacterium tuberculosis comparison standard drug streptomycin. 6 9 showed superb antimicrobial whereas 5 ability. vitro findings on antituberculosis activity reinforced molecular docking study, which become crucial component computational research utilizing enzyme Mtb Pks13 thioesterase domain M. . Additionally, this work, absorption–distribution–metabolism–excretion–toxicity (ADMET) study sparked compounds' drug‐like behavior.

Язык: Английский

---

Impact of the gut-lung axis on tuberculosis susceptibility and progression

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Июль 6, 2023

Tuberculosis (TB) has remained at the forefront of global infectious disease burden for centuries. Concerted efforts to eliminate TB have been hindered by complexity Mycobacterium tuberculosis ( Mtb ), emergence antibiotic resistant strains and recent impact ongoing pandemic coronavirus 2019 (COVID19). Examination immunomodulatory role gastrointestinal microbiota presents a new direction research. The gut microbiome is well-established as critical modulator early immune development inflammatory responses in humans. Recent studies animal models further substantiated existence ‘gut-lung axis’, where distal commensals modulate lung function. This microbiome-lung crosstalk postulated an important correlation with pathophysiology TB. Further evaluation this immunomodulation may provide novel avenue exploration therapeutic targets. mini-review assesses proposed mechanisms which gut-lung axis impacts susceptibility progression. It also examines current anti-TB therapy on effects dysbiosis treatment outcomes. Finally, it investigates targets, particularly use probiotics informs future developments field.

Язык: Английский

---

Exploring the Potential of Fecal Microbiota Transplantation as a Therapy in Tuberculosis and Inflammatory Bowel Disease

Pathogens, Год журнала: 2023, Номер 12(9), С. 1149 - 1149

Опубликована: Сен. 9, 2023

This review explores the potential benefits of fecal microbiota transplantation (FMT) as an adjunct treatment in tuberculosis (TB), drawing parallels from its efficacy inflammatory bowel disease (IBD). FMT has shown promise restoring gut microbial balance and modulating immune responses IBD patients. Considering similarities immunomodulation dysbiosis between TB, this hypothesizes that may offer therapeutic therapy TB. Methods: We conducted a systematic existing literature on highlighting mechanisms implications management both conditions. The findings contribute to understanding FMT’s role TB underscore necessity for future research direction fully leverage clinical applications. Conclusion: integration into comprehensive could potentially enhance outcomes, reduce drug resistance, mitigate side effects conventional therapies. Future endeavors should focus well-designed trials develop guidelines concerning safety short- long-term patients, well assess risks.

Язык: Английский

---

Gut microbiota composition and diversity before, during, and two months after rifamycin-based tuberculosis preventive therapy

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Ноя. 2, 2023

Abstract Tuberculosis (TB) preventive therapy (TPT) is an effective strategy to eliminate TB in low-incidence settings. Shorter TPT regimens incorporating the antimicrobial class of rifamycins are designed improve adherence and completion rates but carry risk modifications gut microbiota. We enrolled six subjects diagnosed with latent infection (LTBI) who accepted initiate TPT. also healthy volunteers unexposed rifamycins. profiled microbiota using 16S rRNA amplicon sequencing (V1-V2 region) document immediate effect rifamycin-based on composition tracked recovery baseline two months after Overall, accounted for 17% variance microbial community dissimilarity. This induced dysbiosis was characterized by a depletion butyrate-producing taxa (Clostridium-XIVa Roseburia) expansion potentially pathogenic within Firmicutes Proteobacteria phyla. Recovery incomplete Robust clinical studies necessary comprehensively catalogue TPT-induced inform strategies mitigate potential long-term sequelae this important control intervention.

Язык: Английский

---