Association of secondary metabolite gene clusters with host specialization in the cereal blast fungusPyricularia oryzae DOI Creative Commons
Khyati Mehta, Jorge C. Navarro-Muñoz,

Sayali Bakore

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 8, 2023

Abstract Fungal plant pathogens constantly evolve and deploy novel peptide metabolite effectors to break down resistance adapt new host plants. The blast fungal pathogen Pyricularia oryzae is a single species subdivided into multiple host-specific lineages that have evolved through gain and/or loss of virulence effector related genes chromosomal rearrangement. Here, we mined 68 genomes P. , belonging six lineages, identify secondary (SM) biosynthetic gene clusters (BGCs) likely associated with potential involved in specialization. A similarity network analysis grouped total 4501 BGCs 283 cluster families (GCFs), based on the content architecture BGCs. While most GCFs were present all two (BGC-O1 BGC-O2) found specifically Oryza lineage one (BGC-TLE) was specific Triticum, Lolium Eleusine hosts. Further phylogenetic relationships between core confirmed BGC-O1, which comprises reducing polyketide synthase (MGG_08236) four putative tailoring genes, only lineage. Importantly, including MGG_08236, from BGC-O1 expressed during pathogenesis. We propose lineage-specific produces specialization rice host. In addition, identified five SM under positive or balancing selection lineage, suggesting role interaction specifically. Our findings highlight importance further mining fungus different cereal

Язык: Английский

Pyricularia oryzae: Lab star and field scourge DOI Creative Commons
Maël Baudin, Marie Le Naour‐‐Vernet, Pierre Gladieux

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(4)

Опубликована: Апрель 1, 2024

Abstract Pyricularia oryzae (syn. Magnaporthe ), is a filamentous ascomycete that causes major disease called blast on cereal crops, as well wide variety of wild and cultivated grasses. Blast diseases have tremendous impact worldwide particularly rice wheat, where the emerged in South America 1980s, before spreading to Asia Africa. Its economic importance, coupled with its amenability molecular genetic manipulation, inspired extensive research efforts aiming at understanding biology evolution. In past 40 years, this plant‐pathogenic fungus has model plant–microbe interactions. review, we focus clarification taxonomy structure species host range determinants. We also discuss recent studies deciphering lifecycle. Taxonomy Kingdom: Fungi , phylum: Ascomycota sub‐phylum: Pezizomycotina class: Sordariomycetes order: Magnaporthales family: Pyriculariaceae genus: Pyricularia. Host P. ability infect Poaceae . It structured into different host‐specialized lineages are each associated few plant genera. The best known cause damage but it can attack other economically important crops such maize, barley, finger millet. Disease symptoms necrotic lesions or bleaching all aerial parts plants, including leaf blades, sheaths, inflorescences (panicles, spikes, seeds). Characteristic leaves diamond‐shaped silver often brown margin whose appearance influenced by numerous factors genotype environmental conditions. USEFUL WEBSITES Resources URL Genomic data repositories http://genome.jouy.inra.fr/gemo/ http://openriceblast.org/ http://openwheatblast.net/ Genome browser for fungi (including ) http://fungi.ensembl.org/index.html Comparative genomics database https://mycocosm.jgi.doe.gov/mycocosm/home T‐DNA mutant http://atmt.snu.kr/ http://www.phi‐base.org/ SNP expression https://fungidb.org/fungidb/app/

Язык: Английский

Процитировано

8
Genome-Wide Profiling of bZIP Transcription Factors and FocbZIP11’s Impact on Fusarium TR4 Pathogenicity DOI Open Access
Yanling Xie, H. C. Huang,

Yile Huo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1452 - 1452

Опубликована: Фев. 9, 2025

The basic leucine zipper (bZIP) transcription factor (TF) family performs diverse functions in fungal processes, including vegetative growth, nutrient utilization, stress responses, and invasion. Despite their importance, little is known about the bZIP members Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), a highly virulent banana pathogen. In this study, we systematically identified 17 bZIPs distributed across 10 Foc TR4 chromosomes classified them into four types based on protein sequences. Phylogenetic analysis of TFs revealed that FocbZIP proteins cluster 12 groups shared species. A cis-element showed each promoter contains at least one type response-related element. Furthermore, RNA-seq RT-qPCR analyses gene expression patterns demonstrated these genes may serve distinct roles during infection. Notably, deletion FocbZIP11 led to reduced heightened sensitivity osmotic, oxidative, cell wall stresses, diminished virulence toward plantlets. Overall, our findings indicate plays critical role abiotic TR4. This study provides foundation for further functional characterization genes, might as promising target RNA-based biopesticide control FWB.

Язык: Английский

Процитировано

0
A Comprehensive and Intricate Dynamics of Aspergillus: Implications, Therapeutic Challenges, and Drug Resistance DOI

Nabajit Kumar Borah,

Yukti Tripathi,

Aditi Parashar

и другие.

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

1
Systematic characterization of the bZIP gene family in Colletotrichum siamense and functional analysis of three family members DOI
Hong Yang, Weidong Huang, Songle Fan

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 286, С. 138463 - 138463

Опубликована: Дек. 5, 2024

Язык: Английский

Процитировано

0
Association of secondary metabolite gene clusters with host specialization in the cereal blast fungusPyricularia oryzae DOI Creative Commons
Khyati Mehta, Jorge C. Navarro-Muñoz,

Sayali Bakore

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 8, 2023

Abstract Fungal plant pathogens constantly evolve and deploy novel peptide metabolite effectors to break down resistance adapt new host plants. The blast fungal pathogen Pyricularia oryzae is a single species subdivided into multiple host-specific lineages that have evolved through gain and/or loss of virulence effector related genes chromosomal rearrangement. Here, we mined 68 genomes P. , belonging six lineages, identify secondary (SM) biosynthetic gene clusters (BGCs) likely associated with potential involved in specialization. A similarity network analysis grouped total 4501 BGCs 283 cluster families (GCFs), based on the content architecture BGCs. While most GCFs were present all two (BGC-O1 BGC-O2) found specifically Oryza lineage one (BGC-TLE) was specific Triticum, Lolium Eleusine hosts. Further phylogenetic relationships between core confirmed BGC-O1, which comprises reducing polyketide synthase (MGG_08236) four putative tailoring genes, only lineage. Importantly, including MGG_08236, from BGC-O1 expressed during pathogenesis. We propose lineage-specific produces specialization rice host. In addition, identified five SM under positive or balancing selection lineage, suggesting role interaction specifically. Our findings highlight importance further mining fungus different cereal

Язык: Английский

Процитировано

0