Transcription factor BbCDR1 regulates the orchestration between conidial formation and maturation in the entomopathogenic fungus Beauveria bassiana
Pest Management Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 24, 2025
Abstract
BACKGROUND
The
entomopathogenic
fungus
Beauveria
bassiana
has
been
widely
used
for
pest
biocontrol
with
conidia
serving
as
the
main
active
agents.
Conidial
yield
and
quality
are
two
important
characteristics
in
fungal
development,
however,
regulatory
mechanisms
that
orchestrate
conidial
formation
development
not
well
understood.
RESULTS
In
this
study,
we
identified
a
Zn
2
Cys
6
transcription
factor
BbCDR1
inhibits
production
while
promoting
maturation.
Compared
wild
type,
ΔBbCDR1
mutant
exhibited
1.88‐fold
increase
but
reduction
quality,
including
decreased
cell
wall
integrity
trehalose
synthesis.
deletion
of
also
led
to
reduced
germination
rates
under
oxidative,
osmotic,
disruption
UV
stresses.
Interestingly,
an
virulence
12.5%
decrease
LT
50
compared
type.
CONCLUSIONS
Further
analysis
revealed
represses
expression
BbbrlA
activates
BbwetA
,
components
central
developmental
pathway
(CDP)
regulate
initiation
maturation,
respectively.
These
findings
suggest
plays
crucial
role
new
target
gene
genetic
engineering
highly
insecticides.
©
2025
Society
Chemical
Industry.
Язык: Английский
Effects of microbial biocontrol agents on tea plantation microecology and tea plant metabolism: a review
Yixin Xie,
Chunxia Cao,
Daye Huang
и другие.
Frontiers in Plant Science,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 20, 2025
The
quality
of
fresh
tea
leaves
is
crucial
to
the
final
product,
and
maintaining
microbial
stability
in
plantations
essential
for
optimal
plant
growth.
Unique
communities
play
a
critical
role
shaping
flavor
enhancing
resilience
against
biotic
stressors.
Tea
production
frequently
challenged
by
pests
diseases,
which
can
compromise
both
yield
quality.
While
stress
generally
has
detrimental
effects
on
plants,
it
also
activates
defense
metabolic
pathways,
leading
shifts
communities.
Microbial
biocontrol
agents
(MBCAs),
including
entomopathogenic
antagonistic
microorganisms,
present
promising
alternative
synthetic
pesticides
mitigating
these
stresses.
In
addition
controlling
MBCAs
influence
composition
communities,
potentially
health
resilience.
However,
despite
significant
advances
laboratory
research,
field-level
impacts
microecology
remain
insufficiently
explored.
This
review
provides
insights
into
interactions
among
insects,
offering
strategies
improve
pest
disease
management
plantations.
Язык: Английский
Unlocking Rhizosphere Dynamics: Exploring Mechanisms of Plant–Microbe Interactions for Enhanced Tea (Camellia sinensis (L.) O. Kuntze) Productivity
Current Microbiology,
Год журнала:
2025,
Номер
82(6)
Опубликована: Апрель 22, 2025
Язык: Английский
The effect of population density on the phenotype, metabolic and immunological adaptations in the cuticle of Spodoptera litura larvae
Liang Wen,
Tingting Jin,
X. L. Luo
и другие.
Pest Management Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 8, 2025
Abstract
BACKGROUND
Species
that
experience
outbreaks
and
those
display
density‐dependent
phase
polymorphism
demonstrate
prophylaxis
(DDP)
by
increasing
their
immune
investment
in
response
to
densities.
Despite
this
phenomenon,
the
mechanisms
of
DDP
remain
largely
unexplored.
RESULTS
Here,
we
showed
Spodoptera
litura
exhibited
heightened
cuticular
melanization
enhanced
responses
when
reared
at
higher
population
density.
Transcriptomic
analysis
identified
differentially
expressed
genes
(DEGs)
associated
with
responses,
nutritional
metabolism,
synthesis
cuticle,
revealing
molecular
underpinnings
plasticity
larval
cuticles.
Gregarious
S.
larvae
significant
up‐regulation
immune‐related
genes,
particularly
Toll
deficiency
(IMD)
signaling
pathways
tyrosine
suggesting
a
strategic
enhancement
defenses.
Concurrently,
reduced
lipid
metabolism
was
observed
cuticle
gregarious
larvae,
suppressed
expression
key
fatty
acid
synthesis,
leading
decrease
integument
triglyceride
content.
The
defense
further
amplified
increased
cuticle‐
melanin
formation‐related
chitin
degradation,
reinforcing
as
physical
barrier
against
pathogens.
CONCLUSION
Our
findings
clarify
shifts
phenotypic
plasticity,
metabolic
pathways,
underscore
adaptability
insects
density
changes
consequent
vulnerability
pathogens,
offering
new
directions
insights
for
uncovering
underlying
pest
enhancing
effectiveness
biological
control
measures
targeting
immunity.
©
2025
Society
Chemical
Industry.
Язык: Английский
Fungal biocontrol agents in the management of soil-borne pathogens, insect pests, and nematodes: Mechanisms and implications for sustainable agriculture
The Microbe,
Год журнала:
2025,
Номер
unknown, С. 100391 - 100391
Опубликована: Май 1, 2025
Язык: Английский
Engineered fungus containing a caterpillar gene kills insects rapidly by disrupting their ecto- and endo-microbiomes
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Авг. 7, 2024
Similar
to
the
physiological
importance
of
gut
microbiomes,
recent
works
have
shown
that
insect
ectomicrobiotas
can
mediate
defensive
colonization
resistance
against
fungal
parasites
infect
via
cuticle
penetration.
Here
we
show
engineering
entomopathogenic
fungus
Metarhizium
robertsii
with
a
potent
antibacterial
moricin
gene
from
silkworms
substantially
enhances
ability
kill
mosquitos,
locusts,
and
two
Drosophila
species.
Further
use
melanogaster
as
an
infection
model,
quantitative
microbiome
analysis
reveals
engineered
strains
designed
suppress
cuticular
bacteria
additionally
disrupt
microbiomes.
An
overgrowth
harmful
such
opportunistic
pathogens
Providencia
species
is
detected
accelerate
death.
In
support,
antimicrobial
genes
in
fly
fat
bodies
guts
indicates
topical
infections
result
compromise
intestinal
immune
responses.
addition
providing
innovative
strategy
for
improving
potency
mycoinsecticides,
our
data
solidify
both
ecto-
endo-microbiomes
maintaining
wellbeing.
Язык: Английский
Conserved signaling modules regulate filamentous growth in fungi: a model for eukaryotic cell differentiation
Genetics,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 6, 2024
Abstract
Eukaryotic
organisms
are
composed
of
different
cell
types
with
defined
shapes
and
functions.
Specific
produced
by
the
process
differentiation,
which
is
regulated
signal
transduction
pathways.
Signaling
pathways
regulate
differentiation
sensing
cues
controlling
expression
target
genes
whose
products
generate
specific
attributes.
In
studying
how
cells
differentiate,
fungi
have
proved
valuable
models
because
their
ease
genetic
manipulation
striking
morphologies.
Many
fungal
species
undergo
filamentous
growth—a
specialized
growth
pattern
where
produce
elongated
tube-like
projections.
Filamentous
promotes
expansion
into
new
environments,
including
invasion
plant
animal
hosts
pathogens.
The
same
signaling
that
in
also
control
throughout
eukaryotes
include
highly
conserved
mitogen-activated
protein
kinase
(MAPK)
pathways,
focus
this
review.
many
species,
mucin-type
sensors
MAPK
to
response
diverse
stimuli.
Once
activated,
reorganize
polarity,
induce
changes
adhesion,
promote
secretion
degradative
enzymes
mediate
access
environments.
However,
pathway
regulation
complicated
related
can
share
components
each
other
yet
unique
responses
(i.e.
specificity).
addition,
function
integrated
networks
regulatory
integration).
Here,
we
discuss
specificity
integration
several
yeast
(mainly
Saccharomyces
cerevisiae
Candida
albicans)
focusing
on
filamentation
pathway.
Because
strong
evolutionary
ties
between
a
deeper
understanding
established
increasingly
reveal
fundamentally
mechanisms
underlying
eukaryotic
differentiation.
Язык: Английский
An iron-binding protein of entomopathogenic fungus suppresses the proliferation of host symbiotic bacteria
Microbiome,
Год журнала:
2024,
Номер
12(1)
Опубликована: Окт. 15, 2024
Entomopathogenic
fungal
infection-induced
dysbiosis
of
host
microbiota
offers
a
window
into
understanding
the
complex
interactions
between
pathogenic
fungi
and
symbionts.
Such
insights
are
critical
for
enhancing
efficacy
mycoinsecticides.
However,
utilization
these
in
pest
control
remains
largely
unexplored.
Язык: Английский
Viral- and fungal-mediated behavioral manipulation of hosts: summit disease
Applied Microbiology and Biotechnology,
Год журнала:
2024,
Номер
108(1)
Опубликована: Окт. 23, 2024
Summit
disease,
in
which
infected
hosts
seek
heights
(gravitropism),
first
noted
modern
times
by
nineteenth-century
naturalists,
has
been
shown
to
be
induced
disparate
pathogens
ranging
from
viruses
fungi.
Infection
results
dramatic
changes
normal
activity
patterns,
and
such
parasite
manipulation
of
host
behaviors
suggests
a
strong
selection
for
convergent
outcomes
albeit
evolved
via
widely
divergent
mechanisms.
The
two
best-studied
examples
involve
subset
viral
fungal
insects
that
induce
"summiting"
hosts.
Summiting
presumably
functions
as
means
increasing
the
dispersal
pathogen,
thus
significantly
fitness.
Here,
we
review
current
advances
our
understanding
viral-
fungal-induced
summit
disease
behavioral
involved.
Viral
genes
implicated
this
process
include
hormone-targeting
ecdysteroid
UDP-glucosyltransferase
(apparently
essential
mediating
some
but
not
all)
protein
tyrosine
phosphatase,
with
light
dependance
implicated.
For
disease-causing
fungi,
though
much
remains
obscure,
targeting
molting,
circadian
rhythms,
sleep,
responses
patterns
appear
Targeting
neuronal
pathways
summit-inducing
fungi
also
appears
production
effector
molecules
secondary
metabolites
affect
muscular,
immune,
and/or
neurological
processes.
It
is
hypothesized
brain
structures,
particularly
Mushroom
Bodies
(no
relation
fungus
itself),
important
olfactory
association
learning
control
locomotor
activity,
are
critical
targets
summiting
during
infection.
This
phenomenon
expands
diversity
microbial
pathogen-interactions
dynamics.
KEY
POINTS:
•
or
height
seeking
(gravitropism)
manipulating
insect
increase
pathogen
dispersal.
Insect
baculoviruses
select
exhibit
evolution
use
molecular
Targets
affecting
behavior
hormones,
feeding,
locomotion,
circadian,
pathways.
Язык: Английский