Neurospine, Год журнала: 2023, Номер 20(2), С. 413 - 414
Опубликована: Июнь 30, 2023
Язык: Английский
Bioactive Materials, Год журнала: 2024, Номер 38, С. 438 - 454
Опубликована: Май 12, 2024
Spinal cord injury (SCI) is a traumatic condition that results in impaired motor and sensory function. Ferroptosis one of the main causes neural cell death loss neurological function spinal cord, ferroptosis inhibitors are effective reducing inflammation repairing SCI. Although human umbilical mesenchymal stem cells (Huc-MSCs) can ameliorate inflammatory microenvironments promote regeneration SCI, their efficacy greatly limited by local microenvironment after Therefore, this study, we constructed drug-release nanoparticle system with synergistic Huc-MSCs inhibitor, which anchored Tz-A6 peptide based on CD44-targeting sequence, combined reactive oxygen species (ROS)-responsive drug nanocarrier mPEG-b-Lys-BECI-TCO at other end for SCI repair. Meanwhile, also modified classic inhibitor Ferrostatin-1 (Fer-1) synthesized new prodrug Feborastatin-1 (Feb-1). The showed treatment regimen significantly inhibited response promoted recovery rats This study developed combination therapy provides strategy construction drug-coordinated system.
Язык: Английский
Процитировано
13
Molecular Neurobiology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 31, 2025
Spinal cord injury (SCI) is a severe central nervous system without effective therapies. PANoptosis involved in the development of many diseases, including brain and spinal injuries. However, biological functions molecular mechanisms PANoptosis-related genes remain unclear. In bioinformatics analysis public data SCI, differentially expressed (DEGs) identified by GSE151371 were hybridized with (PRGs) to obtain (DE-PRGs). Through three machine learning algorithms, we obtained hub genes. Then, constructed functional analysis, drug prediction, regulatory network construction, immune infiltrating cell analysis. Finally, expression gene was verified GSE93561, GSE45376, qRT-PCR above 14 DE-PRGs intersecting 3582 DEGs 46 PRGs. Five key genes, CASP4, GSDMB, NAIP, NLRC4, NLRP3, 3 algorithms. All five enriched phagocytosis mediated FC GAMMA R. The 11 cells significantly different between group human control (HC) group, such as mast gamma delta T cell. transcription factor (TF)-hub contained 10-nodes (4 6 TFs) 8-edges. miRNA-hub consisting 5-nodes (3 2 miRNAs) 3-edges constructed. Moreover, CASP4 predicted 1 small molecule NLRP3 9 drugs. 5 GSE45376 GSE93561 (SCI vs. HC) mice SCI model (Sham SCI). Collectively, (CASP4, NLRP3) associated PANoptosis, providing potential directions for treating injury.
Язык: Английский
Процитировано
0
Extracellular Vesicle, Год журнала: 2024, Номер 3, С. 100044 - 100044
Опубликована: Май 13, 2024
Spinal cord injury (SCI) is the damage to spinal causing paralysis below site compromised patient life quality. Exosomes are nanosized vesicles released during physiological or pathological processes. They present a promising avenue for therapeutic drug delivery due their tissue specificity and biocompatibility, offering enhanced efficacy with reduced accumulation-based toxicity. In SCI, exosomes target inflammation, apoptosis, microglial polarization, autophagy, contributing interventions. This article comprehensively explores pathophysiology of diverse exosome synthesis processes, limitations challenges overcome, potential from various cellular sources in SCI treatment.
Язык: Английский
Процитировано
4
Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(2), С. 70 - 70
Опубликована: Янв. 21, 2025
Spinal cord injury (SCI) involves complex pathological processes that often result in significant and long-term neurological deficits. Increasingly, research has identified microRNA-21 (miR-21) as a pivotal regulator SCI, with studies focusing on its roles inflammation, apoptosis, tissue repair. This review synthesizes current findings miR-21’s involvement post-injury molecular events, emphasizing interactions regulatory targets such Phosphatase Tensin Homolog (PTEN) Programmed Cell Death Protein 4 (PDCD4), well broader effects inflammatory apoptotic signaling pathways. Evidence from both vitro vivo suggests modulating miR-21 influences lesion size, cellular dynamics, functional recovery, highlighting potential therapeutic target for SCI. Nonetheless, the clinical translation of miR-21-based therapies poses challenges, including need to optimize dosages, delivery mechanisms, safety profiles. Further is crucial fully delineate determine feasibility integration into SCI treatment protocols. aims provide comprehensive overview pathology, offering insights mechanisms underlying recovery emerging management enhance outcomes quality life affected patients.
Язык: Английский
Процитировано
0
Acta Neurochirurgica, Год журнала: 2025, Номер 167(1)
Опубликована: Март 24, 2025
To evaluate metformin's efficacy in locomotion recovery, alleviating neuropathic pain, and modulating underlying molecular mechanisms Spinal Cord Injury (SCI) rodent models through a systematic review meta-analysis. We conducted comprehensive literature search across Medline, Embase, Scopus, Web of Science from inception to May 2024. included studies that utilized traumatic SCI treated with metformin versus untreated controls. Data on locomotor related secondary injury were extracted. Standardized mean differences (SMDs) synthesized as the pooled effect sizes. Twenty-three comprising 1,567 animals met inclusion criteria. Metformin significantly enhanced function (SMD = 2.23, 95% CI: 1.74, 2.73, p < 0.001) improved both mechanical allodynia 1.18; CI, 0.35 2.00; 0.005) thermal hyperalgesia 2.40; 1.65 3.16; 0.001). It reduces inflammation, oxidative stress, microglial activation, astrogliosis promotes myelination autophagy flux via activating adenosine monophosphate-activated protein kinase (AMPK) signaling pathway. This resulted decreased apoptosis lesion size increased tissue preservation neuronal survival. Subgroup analyses indicated greater improvements when was administered acute (< 3 days injury) phase (meta-regression coefficient 1.65; 0.37 2.93; 0.011). shows significant therapeutic benefits for models, promoting recovery pain. These results underscore its translational potential clinical management.
Язык: Английский
Процитировано
0
ACS Biomaterials Science & Engineering, Год журнала: 2025, Номер unknown
Опубликована: Апрель 11, 2025
Liver disorders like hepatitis, cirrhosis, and hepatocellular carcinoma present a significant global health challenge, with high morbidity mortality rates. Key factors contributing to liver include inflammation, oxidative stress, apoptosis. Due their multifaceted action, natural compounds are promising candidates for mitigating liver-related disorders. Research studies revealed the antioxidant, anti-inflammatory, detoxifying properties of curcumin, glycyrrhizin, silymarin potential detoxification protection. With advancements in nanotechnology drug delivery, have improved stability targetability, thereby enhancing bioavailability therapeutic efficiency. Further, recent genomics an increased understanding genetic influencing hepatoprotective effects agents made way personalized medicine. Moreover, combinatorial therapy products, synthetic drugs, or other has outcomes. Even though clinical trials confirmed efficiency as agents, several challenges remain unanswered translation practice. Therefore, it is logical integrate further advance hepatoprotection. This review gives overview substantial progress field hepatoprotection, specific emphasis on integration genomics. provides valuable insights future research innovations developing strategies
Язык: Английский
Процитировано
0
Frontiers in Cellular Neuroscience, Год журнала: 2024, Номер 17
Опубликована: Янв. 16, 2024
Background Apoptosis after spinal cord injury (SCI) plays a pivotal role in the secondary mechanisms, which cause ultimate neurologic insults. A better understanding of molecular and cellular basis apoptosis SCI allows for improved glial neuronal survival via administrations anti-apoptotic biomarkers. The knowledge structure, development trends, research hotspots have not yet been systematically investigated. Methods Articles reviews on SCI, published from 1st January 1994 to Oct 2023, were retrieved Web Science™. Bibliometrix R was used evaluate annual publications, countries, affiliations, authors, sources, documents, key words, hot topics. Results total 3,359 publications accordance with criterions obtained, exhibited an ascending trend publications. most productive countries USA China. Journal Neurotrauma impactive journal; Wenzhou Medical University prolific affiliation; Cuzzocrea S influential author. “Apoptosis,” “spinal-cord-injury,” “expression,” “activation,” “functional recovery” frequent words. Additionally, “transplantation,” “mesenchymal stemness-cells,” “therapies,” “regeneration,” “repair,” “autophagy,” “exosomes,” “nlrp3 inflammasome,” “neuroinflammation,” “knockdown” latest emerging may inform hottest themes. Conclusions neurological damages. Development novel treatments mainly depends apoptosis-related mechanisms levels. Such therapeutic interventions involve application agents, free radical scavengers, as well anti-inflammatory drugs, can be targeted inhibit core events cascades pathway.
Язык: Английский
Процитировано
3
Pharmaceutical Medicine, Год журнала: 2023, Номер 37(6), С. 463 - 490
Опубликована: Сен. 12, 2023
Язык: Английский
Процитировано
8
Life Sciences, Год журнала: 2023, Номер 334, С. 122193 - 122193
Опубликована: Окт. 20, 2023
Язык: Английский
Процитировано
8
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(20), С. 15285 - 15285
Опубликована: Окт. 18, 2023
Spinal cord injury (SCI) harms patients' health and social economic well-being. Unfortunately, fully effective therapeutic strategies have yet to be developed treat this disease, affecting millions worldwide. Apoptosis autophagy are critical cell death signaling pathways after SCI that should targeted for early interventions mitigate their adverse effects promote functional recovery. Tibolone (TIB) is a selective tissue estrogen activity regulator (STEAR) with neuroprotective properties demonstrated in some experimental models. This study aimed investigate the effect of TIB on apoptotic verify whether promotes motor function A moderate contusion was produced at thoracic level 9 (T9) male Sprague Dawley rats. Subsequently, animals received daily dose orally were sacrificed 1, 3, 14 or 30 days post-injury. Tissue samples collected morphometric immunofluorescence analysis identify damage percentage neurons site. Autophagic (Beclin-1, LC3-I/LC3-II, p62) (Caspase 3) markers also analyzed via Western blot. Finally, assessed using BBB scale. administration significantly increased amount preserved (p < 0.05), improved recovery 0.001) modulated expression time-dependent manner while consistently inhibiting apoptosis 0.05). Therefore, could alternative SCI.
Язык: Английский
Процитировано
4