Acta Pharmaceutica Sinica B,
Год журнала:
2023,
Номер
13(12), С. 5107 - 5120
Опубликована: Июнь 20, 2023
Oxidative
stress
injury
and
mitochondrial
dysfunction
are
major
obstacles
to
neurological
functional
recovery
after
ischemic
stroke.
The
development
of
new
approaches
simultaneously
diminish
oxidative
resist
is
urgently
needed.
Inspired
by
the
overproduced
reactive
oxygen
species
(ROS)
at
neuron
mitochondria,
multifunctional
nanoparticles
with
ROS-responsiveness
mitochondrial-targeted
(SPNPs)
were
engineered,
achieving
specific
targeting
delivery
controllable
drug
release
penumbra.
Due
nose-to-brain
pathway,
SPNPs
which
encapsulated
in
a
thermo-sensitive
gel
intranasal
administration
directly
delivered
penumbra
bypassing
blood‒brain
barrier
(BBB)
enhancing
efficiency.
potential
for
stroke
treatment
was
systematically
evaluated
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
162, С. 114581 - 114581
Опубликована: Март 24, 2023
Puerarin
is
an
isoflavone
compound
derived
from
Pueraria
lobata
in
traditional
Chinese
medicine.
Accumulating
evidence
has
indicated
that
puerarin
demonstrates
multiple
pharmacological
effects
and
exhibits
treatment
potential
for
various
neurological
disorders.
Based
on
the
latest
research
progress
as
a
neuroprotective
agent,
its
activity,
molecular
mechanism,
therapeutic
application
were
systematically
reviewed
with
emphasis
pre-clinical
studies.
The
related
information
was
extracted
compiled
major
scientific
databases,
including
PubMed,
ScienceDirect,
SpringerLink,
National
Knowledge
Infrastructure,
using
'Puerarin',
'Neuroprotection',
'Apoptosis',
'Autophagy',
'Antioxidant',
'Mitochondria',
'Anti-inflammation'
keywords.
This
review
complied
Preferred
Reporting
Items
Systematic
Reviews
criteria.
Forty-three
articles
met
established
inclusion
exclusion
shown
against
variety
of
disorders,
ischemic
cerebrovascular
disease,
subarachnoid
hemorrhage,
epilepsy,
cognitive
traumatic
brain
injury,
Parkinson's
Alzheimer's
anxiety,
depression,
diabetic
neuropathy,
neuroblastoma/glioblastoma.
anti-apoptosis,
proinflammatory
mediator
inhibitory,
autophagy
regulatory,
anti-oxidative
stress,
mitochondria
protection,
Ca2+
influx
anti-neurodegenerative
activities.
exerts
noticeable
models
disorders
vivo
(animal).
will
contribute
to
development
novel
clinical
drug
candidate
However,
well-designed,
high-quality,
large-scale,
multicenter
randomized
studies
are
needed
determine
safety
utility
patients
Frontiers in Pharmacology,
Год журнала:
2023,
Номер
14
Опубликована: Май 22, 2023
Introduction:
Puerarin
(PUE)
is
a
natural
compound
isolated
from
Puerariae
Lobatae
Radix,
which
has
neuroprotective
effect
on
IS.
We
explored
the
therapeutic
and
underlying
mechanism
of
PUE
cerebral
I/R
injury
by
inhibiting
oxidative
stress
related
to
PI3K/Akt/Nrf2
pathway
in
vitro
vivo.
Methods:
The
middle
artery
occlusion
reperfusion
(MCAO/R)
rats
oxygen-glucose
deprivation
(OGD/R)
were
selected
as
models,
respectively.
was
observed
using
triphenyl
tetrazolium
hematoxylin-eosin
staining.
Tunel-NeuN
staining
Nissl
quantify
hippocampal
apoptosis.
reactive
oxygen
species
(ROS)
level
detected
flow
cytometry
immunofluorescence.
Biochemical
method
detect
levels.
protein
expression
Western
blotting.
Finally,
co-immunoprecipitation
used
study
molecular
interaction
between
Keap1
Nrf2.
Results:In
vivo
studies
showed
that
improved
neurological
deficits
rats,
well
decreased
stress.
Immunofluorescence
indicated
release
ROS
can
be
inhibited
PUE.
In
addition,
blotting
results
promoted
phosphorylation
PI3K
Akt,
enabled
Nrf2
enter
nucleus,
further
activated
downstream
antioxidant
enzymes
such
HO-1.
combination
with
inhibitor
LY294002
reversed
these
results.
Nrf2-Keap1
complex
dissociation.
Discussion:
Taken
together,
activate
via
PI3K/Akt
promote
enzyme
expression,
could
ameliorate
stress,
against
I/R-induced
Neuron
injury.
Osteonecrosis
of
the
femoral
head
(ONFH)
is
recognized
as
a
common
refractory
orthopedic
disease
that
causes
severe
pain
and
poor
quality
life
in
patients.
Puerarin
(Pue),
natural
isoflavone
glycoside,
can
promote
osteogenesis
inhibit
apoptosis
bone
mesenchymal
stem
cells
(BMSCs),
demonstrating
its
great
potential
treatment
osteonecrosis.
However,
low
aqueous
solubility,
fast
degradation
vivo,
inadequate
bioavailability,
limit
clinical
application
therapeutic
efficacy.
Tetrahedral
framework
nucleic
acids
(tFNAs)
are
promising
novel
DNA
nanomaterials
drug
delivery.
In
this
study,
tFNAs
Pue
carriers
used
synthesized
tFNA/Pue
complex
(TPC)
exhibited
better
stability,
biocompatibility,
tissue
utilization
than
free
Pue.
A
dexamethasone
(DEX)-treated
BMSC
model
vitro
methylprednisolone
(MPS)-induced
ONFH
vivo
also
established,
to
explore
regulatory
effects
TPC
on
BMSCs.
This
findings
showed
restore
dysfunction
attenuated
induced
by
high-dose
glucocorticoids
(GCs)
through
hedgehog
Akt/Bcl-2
pathways,
contributing
prevention
GC-induced
rats.
Thus,
for
other
osteogenesis-related
diseases.
Abstract
Traditional
Chinese
Medicine
(TCM)
is
widely
used
in
clinical
practice
to
treat
diseases
related
central
nervous
system
(CNS)
damage.
However,
the
blood‐brain
barrier
(BBB)
constitutes
a
significant
impediment
effective
delivery
of
TCM,
thus
substantially
diminishing
its
efficacy.
Advances
nanotechnology
and
applications
TCM
(also
known
as
nano‐TCM)
can
deliver
active
ingredients
or
components
across
BBB
targeted
brain
region.
This
review
provides
an
overview
physiological
pathological
mechanisms
systematically
classifies
common
CNS
types
nanocarriers
that
effectively
brain.
Additionally,
drug
strategies
for
nano‐TCMs
utilize
vivo
properties
vitro
devices
bypass
cross
are
discussed.
further
focuses
on
application
treatment
various
diseases.
Finally,
this
article
anticipates
design
strategy
with
higher
efficiency
probes
their
potential
treating
wider
range
Processes,
Год журнала:
2025,
Номер
13(2), С. 350 - 350
Опубликована: Янв. 27, 2025
Puerarin
(daidzein-8-C-glucoside),
one
of
the
bioactive
isoflavones,
has
attracted
attention
in
various
industries
due
to
its
excellent
pharmacological
effects
such
as
antioxidant
effect,
estrogen-like
activity,
reduction
blood
sugar,
and
neuroprotective
effect.
is
most
abundantly
found
roots
Pueraria
lobata
(RPL)
among
biomass
sources.
To
improve
utilization
feasibility
puerarin,
a
high-yield
extraction
process
should
be
designed
for
RPL.
This
study
aimed
optimize
more
efficiently
recover
puerarin
from
RPL
while
using
generally
recognized
safe
solvents
solvents,
considering
potential
industrial
applications
puerarin.
The
variables
were
optimized
by
one-factor-at-a-time
method,
response
surface
methodology,
time
profiling
study.
As
result,
yield
was
achieved
at
60.56
mg/g
under
optimal
conditions
(ethanol
concentration
46.06%,
temperature
65.02
°C,
ratio
solvent
11.50
mL/g,
22
min).
High
this
contributed
improving
applicability
Frontiers in Pharmacology,
Год журнала:
2022,
Номер
13
Опубликована: Окт. 24, 2022
Neurological
diseases
impose
a
tremendous
and
increasing
burden
on
global
health,
there
is
currently
no
curative
agent.
Puerarin,
natural
isoflavone
extracted
from
the
dried
root
of
Pueraria
montana
var.
Lobata
(Willd.)
Sanjappa
Predeep,
an
active
ingredient
with
anti-inflammatory,
antioxidant,
anti-apoptotic,
autophagy-regulating
effects.
It
has
great
potential
in
treatment
neurological
other
diseases.
Phosphatidylinositol
3-kinases/protein
kinase
B
(PI3K/Akt)
signal
pathway
crucial
transduction
mechanism
that
regulates
biological
processes
such
as
cell
regeneration,
apoptosis,
cognitive
memory
central
nervous
system,
closely
related
to
pathogenesis
system
Accumulating
evidence
suggests
excellent
neuroprotective
effect
puerarin
may
be
regulation
PI3K/Akt
pathway.
Here,
we
summarized
main
functions
effects
via
activating
This
paper
illustrates
puerarin,
agent,
can
protect
nerve
cells
delay
progression
through
