Collagen peptides from sturgeon swim bladder prolong the lifespan and healthspan in Caenorhabditis elegans

Journal of the Science of Food and Agriculture, Год журнала: 2024, Номер 104(9), С. 5244 - 5251

Опубликована: Фев. 3, 2024

Sturgeon is a popular aquaculture species in many countries. Its swim bladder rich collagen but has not yet been exploited scientifically.

Язык: Английский

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Dendrobium Nobile Alcohol Extract Extends the Lifespan of Caenorhabditis elegans via hsf-1 and daf-16

Molecules, Год журнала: 2024, Номер 29(4), С. 908 - 908

Опубликована: Фев. 19, 2024

Dendrobium nobile is a traditional Chinese herb with anti-inflammatory, antioxidant, and neuroprotective properties. However, its antiaging effects are unclear. Herein, we studied the aging-related functions mechanism of action alcohol extract (DnAE) in model organism Caenorhabditis elegans. The results indicated that 1 mg/mL DnAE slowed lipofuscin accumulation, decreased levels reactive oxygen species, elevated superoxide dismutase activity, enhanced oxidative heat stress resistance, extended lifespan nematodes, protected their dopamine neurons from 6-hydroxydopamine-induced neurodegeneration, reduced Aβ-induced neurotoxicity. upregulated mRNA expression transcription factors DAF-16 HSF-1, promoted nuclear localization DAF-16, fluorescence intensity HSP-16.2. it had no effect on mutants. Thus, can significantly extend lifespan, enhance tolerance, delay age-related diseases through DAF-16-dependent pathway.

Язык: Английский

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Splicing accuracy varies across human introns, tissues, age and disease

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 27, 2025

Abstract Alternative splicing impacts most multi-exonic human genes. Inaccuracies during this process may have an important role in ageing and disease. Here, we investigate accuracy using RNA-sequencing data from >14k control samples 40 body sites, focusing on split reads partially mapping to known transcripts annotation. We show that inaccuracies occur at different rates across introns tissues are affected by the abundance of core components spliceosome assembly its regulators. find age is positively correlated with a global decline fidelity, mostly affecting genes implicated neurodegenerative diseases. support for latter observing genome-wide increase Alzheimer’s disease as compared neurologically normal individuals. In work, provide in-depth characterisation accuracy, implications our understanding disorders.

Язык: Английский

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Anti-aging effect of recombinant Morchella eximia immunomodulatory protein on Caenorhabditis elegans

Journal of Functional Foods, Год журнала: 2025, Номер 126, С. 106709 - 106709

Опубликована: Фев. 26, 2025

Язык: Английский

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An Exploration of the Chemical Signals and Neural Pathways Driving the Attraction of Meloidogyne incognita and Caenorhabditis elegans to Favorable Bacteria

Agronomy, Год журнала: 2025, Номер 15(3), С. 590 - 590

Опубликована: Фев. 27, 2025

Root-knot nematodes (RKNs: Meloidogyne sp.) are among the most devastating plant pathogens. Their chemotaxis-driven host-seeking activity is critical for RKNs’ infection success. Using attractants derived from bacterial volatile organic compounds (BVOCs) to interrupt promising management of RKNs. However, little known about how BVOCs contribute attractiveness Here, we provide a first evaluation attractive potential taxonomically diverse bacteria different environments and assemble previously unidentified repertoire bi-attractive M. incognita Caenorhabditis elegans. We found that attraction strength preferred was positively correlated with abundance amounts ketones in BVOC profiles. This suggested key BVOC-mediated attraction. In our behavioral experiments using ketone compounds, evidence arises specifically odor cues, phenomenon not reported previously. study demonstrates time specific blend naturally occurring within profiles serves as odorant regulating their enhanced toward nematodes. used genetic methods show AWCON neurons important worms sense drive these bacteria. Our may serve platform better understanding chemical neuronal basis ketone-mediated bacteria–nematode interactions development new management.

Язык: Английский

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Evaluation and normalization of a set of reliable reference genes for quantitative sgk-1 gene expression analysis in Caenorhabditis elegans -focused cancer research

Nucleosides Nucleotides & Nucleic Acids, Год журнала: 2024, Номер unknown, С. 1 - 20

Опубликована: Фев. 15, 2024

Multiple signaling pathways have been discovered to play a role in aging and longevity, including the insulin/IGF-1 system, AMPK pathway, TOR signaling, JNK germline signaling. Mammalian serum glucocorticoid-inducible kinase 1 (sgk-1), which has associated with various disorders hypertension, obesity, tumor growth, limits survival C. elegans by reducing DAF-16/FoxO activity while suppressing FoxO3 human cell culture. provides significant protection for number of genes cancer. The best known these are lin-35/pRb (mammalian ortholog pRb) CEP-1 p53) genes. Therefore, this study, we aimed investigate expression analyzes sgk-1, is overexpressed many types mammalian cancer, mutant lin-35 demonstrate validation reference wild-type N2 elegans-focused cancer research. To develop functional genomic studies elegans, evaluated stability five candidate (act-1, ama-1, cdc-42, pmp-3, iscu-1) quantitative real-time PCR using algorithms (geNorm, NormFinder, Delta Ct method, BestKeeper, RefFinder) worms. According our findings, act-1 cdc-42 were effective accurately normalizing levels gene lin-35. also displayed most consistent patterns, therefore they utilized standardize level sgk-1. Furthermore, results clearly showed that sgk-1 was upregulated worms compared Our highlight importance definitive mostly expressed

Язык: Английский

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Brief guide to senescence assays using cultured mammalian cells

Molecules and Cells, Год журнала: 2024, Номер 47(9), С. 100102 - 100102

Опубликована: Июль 23, 2024

Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present brief guide to mammalian assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, expression secretory phenotype (SASP). This work will be useful for biologists minimum expertise assays.

Язык: Английский

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Meta-analysis of the transcriptome identifies aberrant RNA processing as common feature of aging in multiple species

Molecules and Cells, Год журнала: 2024, Номер 47(4), С. 100047 - 100047

Опубликована: Март 18, 2024

Aging is accompanied by the gradual deregulation of transcriptome. However, whether age-dependent changes in transcriptome are evolutionarily conserved or diverged remains largely unexplored. Here, we performed a meta-analysis examining using publicly available datasets eleven representative metazoans, ranging from Caenorhabditis elegans to human. To identify transcriptomic associated with aging, analyzed various aspects transcriptome, including genome composition, RNA processing, and functional consequences. The use introns novel splice sites tended increase age, particularly brain. In addition, our analysis suggests that accumulation premature termination codon-containing transcripts common feature aging across multiple animal species. Using C. as test model, showed several splicing factors age-dependently downregulated were required maintain normal lifespan. Thus, aberrant processing appears be short lifespan

Язык: Английский

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Combinatorial transcriptomic and genetic dissection of insulin/IGF‐1 signaling‐regulated longevity in Caenorhabditis elegans

Aging Cell, Год журнала: 2024, Номер 23(7)

Опубликована: Март 26, 2024

Abstract Classical genetic analysis is invaluable for understanding the interactions underlying specific phenotypes, but requires laborious and subjective experiments to characterize polygenic quantitative traits. Contrarily, transcriptomic enables simultaneous objective identification of multiple genes whose expression changes are associated with phenotypes. Here, we conducted crucial longevity using datasets daf‐2 /insulin/IGF‐1 receptor mutant Caenorhabditis elegans . Our unraveled epistatic relationships at level, in addition verifying genetically established interactions. combinatorial also revealed conferred by mutations. In particular, demonstrated that extent lifespan caused various alleles transcription factor daf‐16 / FOXO matched their effects on mutants. We identified aging‐regulating signaling pathways subsets structural functional RNA elements altered different Lastly, elucidated cooperation between several regulators, based combination molecular analysis. These data suggest biological processes coordinately exert networks. Together our work demonstrates utility dissection identifying important physiological processes, including aging longevity.

Язык: Английский

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Autophagy-dependent splicing control directs translation toward inflammation during senescence

Developmental Cell, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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