Animal Cells and Systems,
Год журнала:
2024,
Номер
29(1), С. 35 - 45
Опубликована: Дек. 24, 2024
Tissue
growth
is
controlled
by
various
signaling
pathways,
such
as
the
insulin/IGF-signaling
(IIS)
pathway.
Although
IIS
activation
regulated
a
complex
regulatory
network,
mechanism
underlying
miRNA-based
regulation
of
pathway
in
Drosophila
wing
development
remains
unclear.
In
this
study,
we
found
that
size
adult
flies
was
negatively
affected
miR-263b
expression.
The
miR-263b-mediated
alteration
linked
to
reduction
cell
number.
Additionally,
overexpression
S2
cells
decreased
proliferation
and
increased
death.
Consequently,
identified
Akt
direct
target
miR-263b-5p
due
changes
Co-expression
miR-263b-overexpressing
wings
rescued
overexpression-mediated
growth.
These
results
enhance
our
understanding
crucial
role
miRNAs
during
development.
Annals of General Psychiatry,
Год журнала:
2025,
Номер
24(1)
Опубликована: Апрель 4, 2025
Schizophrenia
is
a
complex,
heterogeneous,
and
highly
disabling
severe
mental
disorder
whose
pathogenesis
has
not
yet
been
fully
elucidated.
Epigenetics,
as
bridge
between
genetic
environmental
factors,
plays
an
important
role
in
the
pathophysiology
of
schizophrenia.
Over
past
decade,
epigenetic-wide
association
studies
have
rapidly
become
branch
psychiatric
research,
especially
deciphering
molecular
mechanisms
This
review
systematically
analyzes
recent
advances
epigenome-wide
(EWAS)
schizophrenia,
focusing
on
technological
developments.
We
synthesize
findings
from
large-scale
EWAS
alongside
emerging
evidence
DNA
methylation
patterns,
histone
modifications,
regulatory
networks,
emphasizing
their
roles
disease
treatment
responses.
In
addition,
this
provides
prospective
outlook,
evaluating
impact
that
developments
may
future
With
continuous
advancement
high-throughput
sequencing
technology
increasing
maturity
big
data
analysis
methods,
epigenetics
expected
to
significant
early
diagnosis,
prognosis
assessment
even
personalized
Briefings in Functional Genomics,
Год журнала:
2025,
Номер
24
Опубликована: Янв. 1, 2025
Abstract
Non-coding
RNAs
(ncRNAs)
are
RNA
molecules
that
transcribed
from
DNA
but
not
translated
into
proteins.
Studies
over
the
past
decades
have
revealed
ncRNAs
can
be
classified
small
RNAs,
long
non-coding
and
circular
by
genomic
size
structure.
Accumulated
evidences
eludicated
critical
roles
of
these
transcripts
in
regulating
gene
expression
through
transcription
translation,
thereby
shaping
cellular
function
disease
pathogenesis.
Notably,
recent
studies
investigated
as
competitive
endogenous
(ceRNAs)
sequester
miRNAs
modulate
mRNAs
expression.
The
ceRNAs
network
emerges
a
pivotal
regulatory
function,
with
significant
implications
various
diseases
such
cancer
neurodegenerative
disease.
Therefore,
we
highlighted
multiple
bioinformatics
tools
databases
aim
to
predict
interaction.
Furthermore,
discussed
limitations
using
current
technologies
potential
improvement
for
detection.
Understanding
dynamic
interplay
within
may
advance
biological
comprehension,
well
providing
targets
therapeutic
intervention.
Cancer Gene Therapy,
Год журнала:
2024,
Номер
31(6), С. 941 - 954
Опубликована: Март 19, 2024
Abstract
Medulloblastoma
(MB),
a
prevalent
pediatric
central
nervous
system
tumor,
is
influenced
by
microRNAs
(miRNAs)
that
impact
tumor
initiation
and
progression.
However,
the
specific
involvement
of
miRNAs
in
MB
tumorigenesis
remains
unclear.
Using
single-cell
RNA
sequencing,
we
identified
ROR2
expression
normal
human
fetal
cerebellum.
Subsequent
analyses,
including
immunofluorescence,
quantitative
real-time
PCR
(qRT-PCR),
Western
blot,
assessed
tissues
cell
lines.
We
investigated
miR-124-3p
miR-194-5p
their
regulatory
role
through
dual-luciferase
reporter,
qRT-PCR,
western
blot
assays.
Mechanistic
insights
were
gained
functional
assays
exploring
miR-124-3p,
miR-194-5p,
on
growth
vitro
vivo.
observed
significantly
reduced
elevated
High
inversely
correlated
with
overall
survival
WNT
SHH
subgroups
patients.
Functionally,
overexpressing
inhibiting
suppressed
malignant
transformation
vivo
tumorigenicity.
Mechanistically,
synergistically
regulated
ROR2/PI3K/Akt
pathway,
influencing
Our
findings
indicate
function
as
suppressors,
progression
via
axis,
suggesting
key
mechanism
therapeutic
targets
for
Journal of Orthopaedic Surgery and Research,
Год журнала:
2024,
Номер
19(1)
Опубликована: Ноя. 6, 2024
Osteomyelitis
(OM)
is
an
inflammatory
disease
of
bone
infection
and
destruction
characterized
by
dysregulation
homeostasis.
Staphylococcus
aureus
(SA)
has
been
reported
to
be
the
most
common
pathogen
causing
infectious
OM.
Recent
studies
have
demonstrated
that
N6-methyladenosine
(m6A)
regulators
are
associated
with
development
However,
molecular
mechanism
m6A
modifications
in
OM
remains
unclear.
Here,
we
investigated
function
methyltransferase-like
3
(METTL3)-mediated
modification
development.
In
this
study,
human
mesenchymal
stem
cells
(hBMSCs)
were
treated
staphylococcal
protein
A
(SpA),
a
vital
virulence
factor
SA,
construct
cell
models
Firstly,
found
METTL3
was
upregulated
patients
SpA-induced
hBMSCs,
SpA
treatment
suppressed
osteogenic
differentiation
induced
oxidative
stress
injury
hBMSCs.
Functional
experiments
showed
knockdown
alleviated
inhibition
promotion
inflammation
SpA-treated
Furthermore,
METTL3-mediated
miR-320a
expression
promoting
pri-miR-320a
maturation,
mitigating
effects
on
SpA-mediated
differentiation,
responses
can
reversed
miR-320
mimic.
addition,
phosphatidylinositol-4,
5-bisphosphate
3-kinase
catalytic
subunit
alpha
(PIK3CA)
downstream
target
miR-320a,
upregulation
PIK3CA
miR-320a-induced
during
infection.
Finally,
silencing
regulating
miR-320a/PIK3CA
axis.
Taken
together,
our
data
METTL3/m6A/miR-320a/PIK3CA
axis
regulated
stress,
OM,
which
may
provide
new
therapeutic
strategy
for
patients.
