Journal of Developmental Origins of Health and Disease,
Год журнала:
2024,
Номер
15
Опубликована: Янв. 1, 2024
Abstract
The
aim
of
this
study
was
to
analyse
the
expression
genes
related
regulation
energy
metabolism
in
skeletal
muscle
tissue
by
comparing
male
offspring
two
age
groups
[at
110
and
245
postnatal
days
(pnd)]
from
a
mother
with
obesity
induced
high-fat
diet
(-)-epicatechin
(Epi)
administration.
Four
six
different
litters
were
randomly
selected
for
control
[C
mothers
maternal
(MO)]
or
Epi
intervention
groups.
We
evaluated
effect
on
gastrocnemius
analysing
mRNA
protein
levels
Fndc5/irisin,
Pgc-1α,
Ucp3,
Sln.
significantly
increased
Pgc-1α
MO
group
at
pnd
(
p
<
0.036,
vs
.
MO+Epi),
while
pnd,
Fndc5/irisin
MO+Epi
versus
=
0.006).
No
differences
detected
Ucp3
Sln
(including
mRNA)
protein)
among
experimental
In
conclusion,
treatment
Pgc-α
245.
Furthermore,
it
is
suggested
that
flavonoid
model
its
impact
thermogenesis
are
regulated
pathway
than
Fndc5/irisin.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(5), С. 4651 - 4651
Опубликована: Фев. 28, 2023
Only
in
recent
years,
thanks
to
a
precision
medicine-based
approach,
have
treatments
tailored
the
sex
of
each
patient
emerged
clinical
trials.
In
this
regard,
both
striated
muscle
tissues
present
significant
differences
between
two
sexes,
which
may
important
consequences
for
diagnosis
and
therapy
aging
chronic
illness.
fact,
preservation
mass
disease
conditions
correlates
with
survival;
however,
should
be
considered
when
protocols
maintenance
are
designed.
One
obvious
difference
is
that
men
more
than
women.
Moreover,
sexes
differ
inflammation
parameters,
particularly
response
infection
disease.
Therefore,
unsurprisingly,
women
respond
differently
therapies.
review,
we
an
up-to-date
overview
on
what
known
about
skeletal
physiology
disfunction,
such
as
disuse
atrophy,
age-related
sarcopenia,
cachexia.
addition,
summarize
underly
aforementioned
because
pro-inflammatory
cytokines
deeply
affect
homeostasis.
The
comparison
these
three
their
sex-related
bases
interesting
different
forms
atrophy
share
common
mechanisms;
instance,
those
responsible
protein
dismantling
similar
although
differing
terms
kinetics,
severity,
regulatory
mechanisms.
pre-clinical
research,
exploring
sexual
dimorphism
could
highlight
new
efficacious
or
recommend
implementation
existing
one.
Any
protective
factors
discovered
one
exploited
achieve
lower
morbidity,
reduce
severity
disease,
avoid
mortality
opposite
sex.
Thus,
understanding
sex-dependent
responses
pivotal
importance
design
innovative,
tailored,
efficient
interventions.
Physiological Reports,
Год журнала:
2022,
Номер
10(3)
Опубликована: Фев. 1, 2022
Previous
studies
suggest
that
sex
differences
in
lipid
metabolism
exist
with
females
demonstrating
a
higher
utilization
of
lipids
during
exercise,
which
is
mediated
partly
by
increased
muscle
triglycerides.
However,
whether
these
changes
contribute
directly
to
endurance
exercise
performance
unclear.
Therefore,
the
objective
this
study
was
investigate
contribution
substrate
capacity
(EEC)
mice.
Male
and
female
C57BL/6-NCrl
mice
were
subjected
an
EEC
test
until
exhaustion
on
motorized
treadmill.
The
treadmill
set
at
10%
incline,
speed
gradually
from
10.2
m/min
22.2
fixed
intervals
for
up
2.5
h.
Tissues
blood
harvested
immediately
following
EEC.
A
cohort
sedentary,
non-exercised
male
used
as
controls.
Females
outperformed
males
~25%
Serum
levels
both
fatty
acids
ketone
bodies
~50%
end
In
sedentary
mice,
skeletal
triglyceride
content
significantly
greater
compared
males.
Gene
expression
analysis
demonstrated
genes
involved
acid
oxidation
no
associated
glucose
uptake
or
body
oxidation.
findings
have
ability
mobilize
utilize
energy.
Journal of Applied Physiology,
Год журнала:
2023,
Номер
136(1), С. 109 - 121
Опубликована: Ноя. 23, 2023
Skeletal
muscle
is
a
highly
complex
tissue
that
studied
by
scientists
from
wide
spectrum
of
disciplines,
including
motor
control,
biomechanics,
exercise
science,
physiology,
cell
biology,
genetics,
regenerative
medicine,
orthopedics,
and
engineering.
Although
this
diversity
in
perspectives
has
led
to
many
important
discoveries,
historically,
there
been
limited
overlap
discussions
across
fields.
This
misconceptions
oversimplifications
about
biology
can
create
confusion
potentially
slow
scientific
progress
The
purpose
synthesis
paper
bring
together
research
multiple
fields
identify
common
assumptions
related
fiber
type
are
points
concern
clarify.
These
include
Autophagy,
Год журнала:
2024,
Номер
20(10), С. 2121 - 2132
Опубликована: Июль 15, 2024
Skeletal
muscle
plays
a
crucial
role
in
generating
force
to
facilitate
movement.
is
heterogenous
tissue
composed
of
diverse
fibers
with
distinct
contractile
and
metabolic
profiles.
The
intricate
classification
skeletal
exists
on
continuum
ranging
from
type
I
(slow-twitch,
oxidative)
II
(fast-twitch,
glycolytic).
distribution
characteristics
within
between
muscles
profoundly
influences
cellular
signaling;
however,
this
has
not
been
broadly
discussed
as
it
relates
macroautophagy/autophagy.
growing
interest
autophagy
research
underscores
the
necessity
comprehending
interplay
autophagic
responses
among
different
properties,
profiles,
other
related
signaling
processes.
We
recommend
approaching
interpretation
findings
careful
consideration
for
two
key
reasons:
1)
behaviors
or
various
perturbations,
2)
potential
impact
alterations
fiber
profile
observed
outcomes.
This
review
provides
an
overview
response
muscles/fibers
types
Further,
discusses
conditions
diseases
that
may
differentially
affect
muscle.
Finally,
we
provide
points
better
enable
researchers
fine-tune
design
experiments.
Physiological Reports,
Год журнала:
2021,
Номер
9(18)
Опубликована: Сен. 1, 2021
Skeletal
muscle
anatomy
and
physiology
are
sexually
dimorphic
but
molecular
underpinnings
muscle-specificity
not
well-established.
Variances
in
metabolic
health,
fitness
level,
sedentary
behavior,
genetics,
age
make
it
difficult
to
discern
inherent
sex
effects
humans.
Therefore,
mice
under
well-controlled
conditions
were
used
determine
female
male
(n
=
19/sex)
skeletal
fiber
type/size
capillarity
superficial
deep
gastrocnemius
(GA-s,
GA-d),
soleus
(SOL),
extensor
digitorum
longus
(EDL),
plantaris
(PLT),
transcriptome
patterns
also
determined
(GA,
SOL).
Summed
weight
strongly
correlated
with
lean
body
mass
(r2
0.67,
p
<
0.0001,
both
sexes).
Other
phenotypes
muscle-specific:
e.g.,
(higher
density,
GA-s),
myofiber
size
(higher,
EDL),
type
lower
I
II
prevalences,
respectively,
There
broad
differences
transcriptomics,
>6000
(GA)
>4000
(SOL)
mRNAs
differentially-expressed
by
sex;
only
a
minority
of
these
shared
across
GA
SOL.
Pathway
analyses
revealed
ribosome
biology,
transcription,
RNA
processing.
Curation
transcripts
SOL,
literature
datasets
from
humans,
identified
11
genes
that
we
propose
canonical
innate
muscle:
Xist,
Kdm6a,
Grb10,
Oas2,
Rps4x
females)
Ddx3y,
Kdm5d,
Irx3,
Wwp1,
Aldh1a1,
Cd24a
males).
These
those
the
highest
"sex-biased"
expression
our
study
do
contain
estrogen-response
elements
(exception,
Greb1),
subset
proposed
be
regulated
through
androgen
response
elements.
We
hypothesize
sexual
dimorphism
humans
is
triggered
then
maintained
classic
X
inactivation
(Xist,
Y
activation
(Ddx3y,
males),
coincident
engagement
encoded
(Kdm6a)
(Kdm5d)
demethylase
epigenetic
regulators
complemented
modulation
at
some
regions
genome
respond
androgen.
GeroScience,
Год журнала:
2024,
Номер
46(3), С. 3341 - 3360
Опубликована: Янв. 24, 2024
Abstract
Muscle-aging
drives
sarcopenia
and
is
a
major
public
health
issue.
Mice
are
frequently
used
as
model
for
human
muscle-aging,
however,
research
investigating
their
translational
value
limited.
In
addition,
mechanisms
underlying
muscle-aging
may
have
sex-specific
features
in
humans,
but
it
not
yet
assessed
whether
these
recapitulated
mice.
Here,
we
studied
the
effects
of
aging
on
functional,
histological
transcriptional
level
at
multiple
timepoints
male
female
mice
(4,
17,
21
25
months),
with
particular
emphasis
sex-differences.
The
natural
transcriptome
quadriceps
muscle
were
compared
to
humans
pathway
level.
Significant
loss
mass
occurred
late,
months,
both
(-17%,
quadriceps)
(-10%,
young
control
Concomitantly,
found
female,
mice,
slower
movement
speed
aged
groups
(P
<
0.001).
Consistently,
weighted
gene
co-expression
network
analysis
revealed
stronger
association
between
aging-related
reduction
changes
male,
distinctive
last
age
group
(25
which
highlights
necessity
careful
selection
using
model.
Furthermore,
contrasting
more
(4090
vs.
2285
differentially
expressed
genes
respectively).
Subsequently,
related
pathways
characteristic
humans.
conclusion,
our
data
show
that
sex
has
critical
effect
mouse
trajectory,
although
do
necessarily
reflect
differences
observed
trajectory.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(2), С. 847 - 847
Опубликована: Янв. 13, 2022
Obesity
is
a
worrisomely
escalating
public
health
problem
globally
and
one
of
the
leading
causes
morbidity
mortality
from
noncommunicable
disease.
The
epidemiological
link
between
obesity
broad
spectrum
cardiometabolic
disorders
has
been
well
documented;
however,
underlying
pathophysiological
mechanisms
are
only
partially
understood,
effective
treatment
options
remain
scarce.
Given
its
critical
role
in
glucose
metabolism,
skeletal
muscle
increasingly
become
focus
attention
understanding
impaired
insulin
function
associated
metabolic
sequelae.
We
examined
current
evidence
on
relationship
microvascular
dysfunction
resistance
obesity.
A
growing
body
suggest
an
intimate
reciprocal
glucometabolic
physiology.
phenotype
characterized
by
structural
functional
changes
microcirculation
which
contribute
to
disturbed
homeostasis.
Several
interconnected
etiologic
molecular
have
suggested,
including
endothelial
several
factors,
extracellular
matrix
remodelling,
induction
oxidative
stress
immunoinflammatory
phenotype.
further
correlated
currently
available
pharmacological
agents
that
deductive
therapeutic
relevance
explored
mechanisms,
highlighting
potential
clinical
perspective
treatment.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(13), С. 10425 - 10425
Опубликована: Июнь 21, 2023
As
an
organ
system,
skeletal
muscle
is
essential
for
the
generation
of
energy
that
underpins
contraction,
plays
a
critical
role
in
controlling
balance
and
insulin-dependent
glucose
homeostasis,
as
well
vascular
well-being,
regenerates
following
injury.
To
achieve
there
requirement
"cross-talk"
between
myogenic
components
their
regulatory
factors
comprise
muscle.
Accordingly,
this
review
will
describe
following:
[a]
embryonic
cell-signaling
events
important
establishing
cell-lineage,
cross-talk
endothelial
cells
(EC)
precursors
underpinning
development
muscle,
its
vasculature
satellite-stem-cell
(SC)
pool,
EC-SC
maintains
SC
quiescence
localizes
ECs
to
SCs
angio-myogenesis
postnatally;
[b]
vascular-myocyte
actions
insulin
on
vasodilation
capillary
surface
area
uptake
glucose/insulin
by
myofibers
microvascular-myocyte
dysfunction
characterizes
resistance,
diabetes
hypertension,
estrogen
growth
adults;
[c]
utero
fetal
skeletal-muscle
microvascularization
myofiber
hypertrophy
required
metabolic/vascular
homeostasis
after
birth;
[d]
interactions
underpin
regeneration
post-injury;
[e]
Duchenne
muscular
dystrophy.
Physiological Reports,
Год журнала:
2025,
Номер
13(1)
Опубликована: Янв. 1, 2025
Abstract
While
total
RNA
concentrations
putatively
represent
ribosome
content,
there
is
a
need
to
homologize
various
quantification
approaches.
Thus,
([RNA])
provided
through
UV–Vis
spectroscopy
(UV),
fluorometry‐only
(Fluor),
and
fluorometry‐based
microfluidic
chip
electrophoresis
(MFGE)
were
examined
in
C2C12
myotubes
mouse
skeletal
muscle
determine
if
values
aligned
with
[18S
+
28S
rRNA]
(i.e.,
criterion
metric).
A
novel
antibody
cocktail
(termed
RiboAb
)
was
also
tested
compared
these
models.
In
myotubes,
24‐h
IGF‐1
treatments
increased
(~2.0‐fold)
[RNA]
based
on
UV
(~1.9‐fold),
Fluor
(~2.3
fold),
MFGE
(~2.1‐fold).
C57BL/6
mice,
10
days
of
mechanical
overload
(MOV)
elevated
plantaris
(~1.7‐fold)
according
(~1.5‐fold),
(~1.6‐fold),
(~1.8‐fold).
Myotube
levels
significantly
higher
MOV,
respectively,
versus
controls
(1.3‐fold
1.7‐fold,
respectively),
correlated
(
r
=
0.637
0.853,
p
≤
0.005).
UV,
Fluor,
are
seemingly
valid
surrogates
cell/tissue
although
each
method
has
advantages
(e.g.,
ease
use)
disadvantages
magnitudes
bias)
discussed
herein.
Finally,
the
may
this
should
be
further
explored
other