Advancing zoonotic respiratory virus research through the use of organoids
Current Opinion in Virology,
Год журнала:
2024,
Номер
68-69, С. 101435 - 101435
Опубликована: Сен. 1, 2024
Язык: Английский
The 3C (Cell Culture, Computer Simulation, Clinical Trial) Solution for Optimizing the 3R (Replace, Reduction, Refine) Framework during Preclinical Research Involving Laboratory Animals
ACS Pharmacology & Translational Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 9, 2025
Preclinical
research
has
traditionally
utilized
laboratory
animals
to
elucidate
the
safety,
tolerability,
pharmacokinetics,
and
pharmacodynamics
of
new
chemical
entities
prior
human
trials.
The
use
animal
models
been
pivotal
in
advancing
scientific
knowledge
medical
breakthroughs,
contributing
significantly
our
understanding
complex
biological
processes
diseases.
However,
many
promising
treatments
that
have
demonstrated
efficacy
studies
failed
translate
subjects
during
clinical
Consequently,
testing
faces
ethical
concerns
criticism
regarding
its
predictive
reliability
for
responses.
This
led
development
3R
principles
(Replacement,
Reduction,
Refinement),
introduced
1959,
advocating
alternative
methods
improved
welfare
research.
Furthermore,
regulatory
frameworks
recent
legislation,
such
as
2022
FDA
Modernisation
Act,
emphasize
modern
alternatives
traditional
testing.
Emerging
approaches,
known
3Cs-cell
culture,
computer
simulation,
phase
0
trials-offer
nonanimal
solutions
could
accelerate
drug
address
concerns,
potentially
rendering
preclinical
more
humane
efficient.
Язык: Английский
Organoids in Dynamic Culture: Microfluidics and 3D Printing Technologies
ACS Biomaterials Science & Engineering,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 18, 2025
With
the
rapid
advancement
of
biomaterials
and
tissue
engineering
technologies,
organoid
research
its
applications
have
made
significant
strides.
Organoids
are
increasingly
utilized
in
pharmacology,
regenerative
medicine,
precision
clinical
medicine.
Current
trends
moving
toward
multifunctional
composite
three-dimensional
cultivation
dynamic
strategies.
Key
technologies
driving
this
evolution,
including
3D
printing
microfluidics,
continue
to
impact
new
areas
discovery
relevance.
This
review
provides
a
systematic
overview
these
emerging
trends,
discussing
strengths
limitations
critical
offering
insight
directions
for
professionals
working
field.
Язык: Английский
Innovative approaches to blood-brain barrier modelling: The chip revolution
Next Nanotechnology,
Год журнала:
2025,
Номер
7, С. 100171 - 100171
Опубликована: Янв. 1, 2025
Язык: Английский
Interpretation of the past, present, and future of organoid technology: an updated bibliometric analysis from 2009 to 2024
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Авг. 13, 2024
Organoid
technology
has
been
developed
rapidly
in
the
past
decade,
which
involves
exploration
of
mechanism
development,
regeneration
and
various
diseases,
intersects
among
multiple
disciplines.
Thousands
literature
on
3D-culture
or
organoids
have
published
research
areas
cell
biology
tissue
engineering,
nanoscience,
oncology
so
on,
resulting
it
being
challenging
for
researchers
to
timely
summarize
these
studies.
Bibliometric
statistics
is
a
helpful
way
help
clarify
above
issues
efficiently
manage
whole
landscape
systematically.
In
our
study,
all
original
articles
were
included
Web
Science
database
from
January
2009
May
2024,
related
information
was
collected
analyzed
using
Excel
software,
“bibliometrix”
packages
R
VOSviewer
CiteSpace.
As
results,
total
6222
papers
classify
status
quo
predict
future
areas.
Our
findings
highlight
growing
trend
publications
organoids,
with
United
States
Netherlands
leading
this
field.
The
University
California
System,
Harvard
University,
Utrecht
Medical
Center
emerged
as
pivotal
contributors
key
authors
field
include
Clevers,
H,
Beekman,
JM
Spence
JR.
results
also
revealed
that
hotspots
trends
mainly
focused
clinical
treatment,
drug
screening,
application
materials
technologies
such
“hydrogel”
“microfluidic
technology”
organoids.
Next,
we
had
an
in-depth
interpretation
development
process
organoid
area,
including
emergence
technology,
translation
bench
bedsides,
profiles
most
widely
studied
types
technologies,
emerging
organoid-immune
co-cultures
trends.
Furthermore,
discussed
pitfalls,
challenges
prospects
technology.
conclusion,
study
provides
readers
straightforward
convenient
access
Язык: Английский
Transcriptomic and proteomic sequencing unveils the role of vitamin D and metabolic flux shifts in the induction of human hepatic organoids
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Дек. 18, 2024
Hepatic
organoids
(HOs),
validated
through
comparative
sequencing
with
human
liver
tissues,
are
reliable
models
for
research.
Comprehensive
transcriptomic
and
proteomic
of
HOs
throughout
their
induction
period
will
enhance
the
platform's
utility,
aiding
in
elucidation
development's
molecular
mechanisms.
We
developed
hepatic
(HOs)
from
embryonic
stem
cells
(ESCs)
a
de
novo
protocol,
mimicking
stages
fetal
development:
ESCs
to
definitive
endoderm
(DE),
then
foregut
(FG),
hepatoblasts
(HB),
finally
stage
1
(HO1),
culminating
self-organizing
2
(HO2)
via
dissociation
re-inoculation.
The
successful
establishment
was
by
immunofluorescence
staining
RT-qPCR
specific
markers.
analysis
were
conducted
on
FG,
HB,
HO1,
HO2.
Our
data
suggest
that
several
transcription
factors
(TFs)
activated
during
HB
share
overlapping
target
genes
vitamin
D
receptor
(VDR).
Calcitriol,
direct
activator
VDR,
notably
facilitated
FG
transition
activating
VDR
enhancing
key
TFs,
thereby
promoting
progenitor
cell
maturation.
Furthermore,
our
findings
revealed
significant
towards
glycolytic
energy
metabolism
at
HO2
stage,
characterized
increased
flux
reduced
oxidative
phosphorylation.
Inhibition
glycolysis
using
2-deoxy-D-glucose
(2-DG)
led
suppressed
growth
differentiation
stage.
Analysis
signaling
pathways
indicated
upregulation
HIF-1
pathway,
which
is
associated
activation,
as
well
MAPK
PI3K-AKT
pathways,
regulate
HIF-1α
protein
translation.
elucidated
pivotal
role
calcitriol
facilitating
augmenting
expression
critical
(TFs).
Besides,
research
underscores
shift
metabolic
toward
organoids.
Overall,
multiomics
approach
reveals
intricate
regulation
development
HOs.
Язык: Английский
Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 28, 2024
Abstract
Musculoskeletal
diseases
have
emerged
as
the
leading
cause
of
disability
worldwide,
with
their
prevalence
increasing
annually.
In
light
this
escalating
health
challenge,
organoids,
an
emerging
technology
in
tissue
engineering,
offer
promising
solutions
for
disease
modeling,
drug
screening,
regeneration,
and
repair
processes.
The
successful
development
musculoskeletal
organoids
represents
a
significant
breakthrough,
providing
novel
platform
studying
facilitating
discovery
new
treatments.
Moreover,
serve
valuable
complements
to
traditional
2D
culture
methods
animal
models,
offering
rich
insights
into
biology.
This
review
provides
overview
organoid
technology,
outlining
construction
processes
various
highlighting
similarities
differences.
Furthermore,
challenges
associated
systems
are
discussed
future
perspectives
offered.
Язык: Английский
Biomedical applications of organoids in genetic diseases
Medical Review,
Год журнала:
2024,
Номер
5(2), С. 152 - 163
Опубликована: Дек. 24, 2024
Organoid
technology
has
significantly
transformed
biomedical
research
by
providing
exceptional
prospects
for
modeling
human
tissues
and
disorders
in
a
laboratory
setting.
It
significant
potential
understanding
the
intricate
relationship
between
genetic
mutations,
cellular
phenotypes,
disease
pathology,
especially
field
of
diseases.
The
intersection
organoid
offers
great
promise
comprehending
pathophysiology
diseases
creating
innovative
treatment
approaches
customized
specific
patients.
This
review
aimed
to
present
thorough
analysis
current
advancements
its
applications
We
examined
techniques
using
platforms,
analyze
incorporating
organoids
into
clinical
practice,
showcase
breakthroughs
preclinical
application,
individualized
healthcare,
transplantation.
Through
integration
knowledge
from
several
disciplines,
such
as
genetics,
regenerative
medicine,
biological
engineering,
our
aim
is
enhance
comprehension
complex
connection
variations
models
relation
health
disease.
Язык: Английский