Biomedical applications of organoids in genetic diseases DOI Creative Commons
Wenhua Huang, Seogsong Jeong, Won‐Ho Kim

et al.

Medical Review, Journal Year: 2024, Volume and Issue: 5(2), P. 152 - 163

Published: Dec. 24, 2024

Organoid technology has significantly transformed biomedical research by providing exceptional prospects for modeling human tissues and disorders in a laboratory setting. It significant potential understanding the intricate relationship between genetic mutations, cellular phenotypes, disease pathology, especially field of diseases. The intersection organoid offers great promise comprehending pathophysiology diseases creating innovative treatment approaches customized specific patients. This review aimed to present thorough analysis current advancements its applications We examined techniques using platforms, analyze incorporating organoids into clinical practice, showcase breakthroughs preclinical application, individualized healthcare, transplantation. Through integration knowledge from several disciplines, such as genetics, regenerative medicine, biological engineering, our aim is enhance comprehension complex connection variations models relation health disease.

Language: Английский

The 3C (Cell Culture, Computer Simulation, Clinical Trial) Solution for Optimizing the 3R (Replace, Reduction, Refine) Framework during Preclinical Research Involving Laboratory Animals DOI
Sounak Roy,

Mubeena Parveen,

Asis Bala

et al.

ACS Pharmacology & Translational Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Language: Английский

Citations

0
Organoids in Dynamic Culture: Microfluidics and 3D Printing Technologies DOI
Xin Su, Mingqi Wang,

Ruqiang Yuan

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

With the rapid advancement of biomaterials and tissue engineering technologies, organoid research its applications have made significant strides. Organoids are increasingly utilized in pharmacology, regenerative medicine, precision clinical medicine. Current trends moving toward multifunctional composite three-dimensional cultivation dynamic strategies. Key technologies driving this evolution, including 3D printing microfluidics, continue to impact new areas discovery relevance. This review provides a systematic overview these emerging trends, discussing strengths limitations critical offering insight directions for professionals working field.

Language: Английский

Citations

0
Advancing zoonotic respiratory virus research through the use of organoids DOI
Meaghan Flagg, Emmie de Wit

Current Opinion in Virology, Journal Year: 2024, Volume and Issue: 68-69, P. 101435 - 101435

Published: Sept. 1, 2024

Language: Английский

Citations

3
Interpretation of the past, present, and future of organoid technology: an updated bibliometric analysis from 2009 to 2024 DOI Creative Commons

Baozhen Qu,

Qiang Mu,

Huanhuan Bi

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 13, 2024

Organoid technology has been developed rapidly in the past decade, which involves exploration of mechanism development, regeneration and various diseases, intersects among multiple disciplines. Thousands literature on 3D-culture or organoids have published research areas cell biology tissue engineering, nanoscience, oncology so on, resulting it being challenging for researchers to timely summarize these studies. Bibliometric statistics is a helpful way help clarify above issues efficiently manage whole landscape systematically. In our study, all original articles were included Web Science database from January 2009 May 2024, related information was collected analyzed using Excel software, “bibliometrix” packages R VOSviewer CiteSpace. As results, total 6222 papers classify status quo predict future areas. Our findings highlight growing trend publications organoids, with United States Netherlands leading this field. The University California System, Harvard University, Utrecht Medical Center emerged as pivotal contributors key authors field include Clevers, H, Beekman, JM Spence JR. results also revealed that hotspots trends mainly focused clinical treatment, drug screening, application materials technologies such “hydrogel” “microfluidic technology” organoids. Next, we had an in-depth interpretation development process organoid area, including emergence technology, translation bench bedsides, profiles most widely studied types technologies, emerging organoid-immune co-cultures trends. Furthermore, discussed pitfalls, challenges prospects technology. conclusion, study provides readers straightforward convenient access

Language: Английский

Citations

1
Transcriptomic and proteomic sequencing unveils the role of vitamin D and metabolic flux shifts in the induction of human hepatic organoids DOI Creative Commons
Shule Zhang, Linghong Liu, Xianyu Li

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 18, 2024

Hepatic organoids (HOs), validated through comparative sequencing with human liver tissues, are reliable models for research. Comprehensive transcriptomic and proteomic of HOs throughout their induction period will enhance the platform's utility, aiding in elucidation development's molecular mechanisms. We developed hepatic (HOs) from embryonic stem cells (ESCs) a de novo protocol, mimicking stages fetal development: ESCs to definitive endoderm (DE), then foregut (FG), hepatoblasts (HB), finally stage 1 (HO1), culminating self-organizing 2 (HO2) via dissociation re-inoculation. The successful establishment was by immunofluorescence staining RT-qPCR specific markers. analysis were conducted on FG, HB, HO1, HO2. Our data suggest that several transcription factors (TFs) activated during HB share overlapping target genes vitamin D receptor (VDR). Calcitriol, direct activator VDR, notably facilitated FG transition activating VDR enhancing key TFs, thereby promoting progenitor cell maturation. Furthermore, our findings revealed significant towards glycolytic energy metabolism at HO2 stage, characterized increased flux reduced oxidative phosphorylation. Inhibition glycolysis using 2-deoxy-D-glucose (2-DG) led suppressed growth differentiation stage. Analysis signaling pathways indicated upregulation HIF-1 pathway, which is associated activation, as well MAPK PI3K-AKT pathways, regulate HIF-1α protein translation. elucidated pivotal role calcitriol facilitating augmenting expression critical (TFs). Besides, research underscores shift metabolic toward organoids. Overall, multiomics approach reveals intricate regulation development HOs.

Language: Английский

Citations

1
Organoids of Musculoskeletal System for Disease Modeling, Drug Screening, and Regeneration DOI
Wei-Cheng Chen, Dachuan Liu, Kai Lü

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Abstract Musculoskeletal diseases have emerged as the leading cause of disability worldwide, with their prevalence increasing annually. In light this escalating health challenge, organoids, an emerging technology in tissue engineering, offer promising solutions for disease modeling, drug screening, regeneration, and repair processes. The successful development musculoskeletal organoids represents a significant breakthrough, providing novel platform studying facilitating discovery new treatments. Moreover, serve valuable complements to traditional 2D culture methods animal models, offering rich insights into biology. This review provides overview organoid technology, outlining construction processes various highlighting similarities differences. Furthermore, challenges associated systems are discussed future perspectives offered.

Language: Английский

Citations

0
Biomedical applications of organoids in genetic diseases DOI Creative Commons
Wenhua Huang, Seogsong Jeong, Won‐Ho Kim

et al.

Medical Review, Journal Year: 2024, Volume and Issue: 5(2), P. 152 - 163

Published: Dec. 24, 2024

Organoid technology has significantly transformed biomedical research by providing exceptional prospects for modeling human tissues and disorders in a laboratory setting. It significant potential understanding the intricate relationship between genetic mutations, cellular phenotypes, disease pathology, especially field of diseases. The intersection organoid offers great promise comprehending pathophysiology diseases creating innovative treatment approaches customized specific patients. This review aimed to present thorough analysis current advancements its applications We examined techniques using platforms, analyze incorporating organoids into clinical practice, showcase breakthroughs preclinical application, individualized healthcare, transplantation. Through integration knowledge from several disciplines, such as genetics, regenerative medicine, biological engineering, our aim is enhance comprehension complex connection variations models relation health disease.

Language: Английский

Citations

0