Dual-pathway tumor radiosensitization strategy based on engineered bacteria capable of targeted delivery of AuNPs and specific hypoxia alleviation

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 29, 2025

Radiotherapy efficacy remains constrained by two key challenges: dose-dependent toxicity to healthy tissues at high radiation doses and hypoxia-mediated tumor radioresistance. While radiosensitizers like gold nanoparticles can enhance tumor-specific deposition, their targeted delivery tumors presents a significant hurdle. Bacteria have emerged as promising bio-carriers that not only actively target penetrate complex microenvironments, but also be genetically engineered multifunctional platforms for radiosensitizer hypoxia alleviation. An integrated nanosystem (PCM@AuNPs), composed of bacteria (PCM) (AuNPs), is used increase the effectiveness radiotherapy. PCM colonize sites more effectively, thus improving efficiency radiosensitizers. Furthermore, overexpresses catalase (CAT), which decomposes excess H2O2 into O2, helping mitigate in TME. Under X-ray irradiation, PCM@AuNPs significantly radiosensitization, leading improved growth inhibition while maintaining good biocompatibility. effective strategy based on an (PCM@AuNPs) radiosensitization through multiple pathways developed. This novel bacterial holds great promise enhancing cancer therapy.

Язык: Английский

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Nanoengineered Copper: Revolutionizing Breast Cancer Care

International Journal of Scientific Research in Science Engineering and Technology, Год журнала: 2025, Номер 12(1), С. 164 - 180

Опубликована: Фев. 3, 2025

Breast cancer continues to rank as a predominant cause of illness and death among women globally, emphasizing the critical need for innovative effective treatment approaches. Traditional methods such chemotherapy, surgery, radiation therapy often face limitations, including toxicity, resistance, non-specific targeting. In recent years, advancements in nanotechnology have brought about transformative solutions, with nanoengineered copper emerging frontrunner. Nanoengineered copper’s unique properties- its catalytic capabilities, biocompatibility, potential surface modification- offer unprecedented opportunities revolutionize breast care. These nanoparticles not only enable precise targeting cells but also improve diagnostic accuracy through advanced imaging techniques. Furthermore, their application photothermal photodynamic therapies provides dual approach destroying while minimizing harm healthy tissues. With ability modulate tumor microenvironment act platform combination therapies, holds immense promise both early detection treatment. This article explores wide-ranging applications copper, from innovations therapeutic breakthroughs, underscoring redefine management patient outcomes.

Язык: Английский

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Integrative analysis of single-cell and bulk RNA sequencing reveals the oncogenic role of ANXA5 in gastric cancer and its association with drug resistance

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 7, 2025

Background Gastric cancer (GC) remains a leading cause of cancer-related mortality, with over one million new cases and 769,000 deaths reported in 2020. Despite advancements chemotherapy, surgery, targeted therapies, delayed diagnosis due to overlooked early symptoms leads poor prognosis. Methods We integrated bulk RNA sequencing single-cell datasets from TCGA, GEO, OMIX001073, employing normalization, batch effect correction, dimensionality reduction methods identify key cell populations associated GC invasion epithelial-mesenchymal transition (EMT), as well analyze the tumor immune microenvironment. Results Our analysis identified MUC5AC+ malignant epithelial cluster significant player EMT. Cluster 1, representing this population, exhibited higher EMT scores compared other clusters. Survival showed that high abundance 0 correlated improved survival rates (P=0.012), whereas 1 was poorer outcomes (P=0.045). A prognostic model highlighted ANXA5 GABARAPL2 two critical genes upregulated tumors. High-risk patients demonstrated increased infiltration worse prognosic. Analysis mutation burden (TMB) indicated low TMB high-risk group had worst Wet-lab validation experiments confirmed oncogenic role ANXA5, showing its facilitation proliferation, invasion, migration while suppressing apoptosis. Conclusion This study offers novel insights into subpopulations cells their roles progression. It provides potential therapeutic targets combat GC, contributing crucial understanding fundamental mechanisms drug resistance gastrointestinal cancers.

Язык: Английский

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Epigenetic modifications in breast cancer: from immune escape mechanisms to therapeutic target discovery

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 17, 2025

Breast cancer (BC) is one of the most prevalent malignant tumors among women globally, with number cases accounting for even more than 1/3 all tumor patients in women. Recent studies have found that incidence BC increasing every year. Despite great progress made treatment, characteristics cells, such as strong immune evasion, easy recurrence and drug resistance, are still main reasons limiting survival patients. Epigenetics becoming an important method to reveal development cancer, mainly through study DNA methylation, histone modification, chromatin structure changes non-coding RNA. In addition, researchers epigenetic markers potential early detection personalized treatment BC. Inhibitors targeting epigenetically modified enzymes effective treating a wide range provide significant patient quality life. Therefore, this review will comprehensively summarize role modifications development. Second, paper focus on summarizing how induce formation microenvironment (TIME) Targeting mechanism action provides new perspectives unravel complex process development, while paving way novel diagnostic therapeutic targets. future, by integrating multi-omics data enable deeper understanding pathogenesis BC, we be able promote overall precision medicine.

Язык: Английский

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Dendrimer-Entrapped CuPt Bimetallic Nanozymes for Tumor Microenvironment-Regulated Photothermal/Catalytic Therapy

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

Noble metal nanozymes have attracted extensive attention in tumor therapy due to their multiple enzyme-like activities for catalytic and unique optical properties photothermal (PTT). However, therapeutic efficiency may be compromised by the intricate microenvironment (TME). Herein, copper-platinum (CuPt) bimetallic were synthesized entrapped poly(amidoamine) dendrimers, with p-carboxybenzenesulfonamide (BS, a carbonic anhydrase IX (CA IX) inhibitor) modified on surface lonidamine (Lon) loaded inside regulate TME enhanced PTT. The formed CPL@G5-BS could specifically target CA IX-overexpressed cells activate cascade reaction continuous •OH generation peroxidase-like property, O2 supply catalase-like H2O2 replenishing superoxide dismutase-like thereby alleviating hypoxia achieving chemodynamic (CDT). In TME, BS-mediated inhibition would normalize extracellular pH suppress metastasis while reducing intracellular boost efficiency, Lon-mediated mitochondrial respiration energy metabolic disruption elevate oxygen accumulation downregulate heat shock protein (HSP) expression, further enhancing PTT efficacy. Meanwhile, excellent performance of amplify multienzyme activities, Cu2+-mediated glutathione depletion improved CDT efficiency. Overall, can efficiently inhibit growth TME-regulated

Язык: Английский

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Dissecting the dual role of OTU family proteins in tumor progression and immune escape

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Май 21, 2025

As a core mechanism regulating intracellular protein homeostasis, the dynamic equilibrium between ubiquitination and deubiquitination profoundly impacts functionality fate of target proteins. The Ovarian tumor domain (OTU) family, vital subclass deubiquitinating enzymes, comprises 16 members that mediate ubiquitin binding hydrolysis through their characteristic OTU domain. Recent years have witnessed growing interest in family oncology immunology research. This review comprehensively elucidates mechanisms by which regulate tumor-associated signaling networks via substrate-specific deubiquitination. On one hand, they directly govern cell proliferation, metastasis, apoptosis modulating stability key substrates. other orchestrate progression regulation inflammatory intensity, immune response duration, evasion within microenvironment (TME), thereby constructing multidimensional regulatory network development. These findings not only unveil pivotal role tumorigenesis modulation but also establish theoretical foundation for developing novel anti-tumor therapeutics targeting processes. Notably, OTUs emerge as high-potential therapeutic targets with high translational relevance refining precision-guided tumor-immunotherapy integration strategies.

Язык: Английский

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A hypoxia-activated copper ion nanoexchanger for cancer immunotherapy by in situ precise production of immunogenic cell death inducer

Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 111404 - 111404

Опубликована: Июнь 1, 2025

Язык: Английский

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Critical role of neem and jujube extracts in stabilizing copper nanostructures: A comparative study in green synthesis of CuNSs

Current Research in Green and Sustainable Chemistry, Год журнала: 2024, Номер 9, С. 100437 - 100437

Опубликована: Янв. 1, 2024

Язык: Английский

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