Electronics,
Год журнала:
2024,
Номер
13(2), С. 260 - 260
Опубликована: Янв. 5, 2024
In
this
paper,
a
method
of
generating
controllable
spikes
utilizing
symmetric
semiconductor
ring
lasers
(SRLs)
is
investigated,
and
various
optical
behaviors
biological
neurons
are
successfully
emulated
on
faster
timescale.
We
demonstrate
the
synchronized
spike
phenomena
in
two
directions,
generated
both
clockwise
(CW)
counterclockwise
(CCW)
modes
tunable
laser
(TL)-injected
SRL.
The
size
peaks
interval
between
them
can
be
manipulated
by
adjusting
output
complex
amplitude
TL
bias
current.
At
same
time,
we
also
analyzed
CW
mode
TL-injected
SRL
replicated
four
distinct
discharge
patterns
neurons.
These
findings
offer
promising
prospects
for
future
neuromorphic
research.
eNeuro,
Год журнала:
2020,
Номер
7(5), С. ENEURO.0060 - 20.2020
Опубликована: Авг. 14, 2020
Genetically
encoded
voltage
indicators
(GEVIs)
could
potentially
be
used
for
mapping
neural
circuits
at
the
plane
of
synaptic
potentials
and
plateau
potentials—two
blind
spots
GCaMP-based
imaging.
In
last
year
alone,
several
laboratories
reported
significant
breakthroughs
in
quality
GEVIs
efficacy
imaging
equipment.
One
major
obstacle
using
well
performing
pursuit
interesting
biological
data
is
process
transferring
between
laboratories,
as
their
qualities
(e.g.,
membrane
targeting,
brightness,
sensitivity,
optical
signal
quality)
are
often
difficult
to
reproduce
outside
laboratory
GEVI
origin.
We
have
tested
eight
available
(Archon1,
ArcLightD,
ASAP1,
ASAP2s,
ASAP3b,
Bongwoori-Pos6,
FlicR1,
chi-VSFP-Butterfly)
two
voltage-sensitive
dyes
(BeRST1
di-4-ANEPPS).
same
microscope,
lens,
detector,
while
light
sources
were
interchanged.
was
attempted
following
three
preparations:
(1)
cultured
neurons,
(2)
HEK293
cells,
(3)
mouse
brain
slices.
Systematic
measurements
successful
only
cells
Despite
differences
brightness
dynamic
response
(ON
rate),
all
produced
reasonable
signals
slices
solid
vitro
properties,
range
initially
by
creator
laboratories.
Side-by-side
comparisons
organic
obtained
a
“third
party”
(current
data)
will
useful
determining
right
indicator
given
research
application.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2018,
Номер
unknown
Опубликована: Окт. 30, 2018
Pose
estimation
is
crucial
for
many
applications
in
neuroscience,
biomechanics,
genetics
and
beyond.
We
recently
presented
a
highly
efficient
method
markerless
pose
based
on
transfer
learning
with
deep
neural
networks
called
DeepLabCut.
Current
experiments
produce
vast
amounts
of
video
data,
which
challenges
both
storage
analysis.
Here
we
improve
the
inference
speed
DeepLabCut
by
up
to
tenfold
benchmark
these
updates
various
CPUs
GPUs.
In
particular,
depending
frame
size,
poses
can
be
inferred
offline
at
1200
frames
per
second
(FPS).
For
instance,
278
×
images
processed
225
FPS
GTX
1080
Ti
graphics
card.
Furthermore,
show
that
robust
standard
compression
(ffmpeg).
Compression
rates
greater
than
1,000
only
decrease
accuracy
about
half
pixel
(for
640
480
size).
DeepLabCut’s
robustness
save
time
hardware
expenses.
The Journal of Comparative Neurology,
Год журнала:
2019,
Номер
527(13), С. 2200 - 2211
Опубликована: Янв. 12, 2019
Identification
and
delineation
of
brain
regions
in
histologic
mouse
sections
is
especially
pivotal
for
many
neurogenomics,
transcriptomics,
proteomics,
connectomics
studies,
yet
this
process
prone
to
observer
error
bias.
Here
we
present
a
novel
navigation
system,
named
NeuroInfo,
whose
general
principle
similar
that
global
positioning
system
(GPS)
car.
NeuroInfo
automatically
navigates
an
investigator
through
the
complex
microscopic
anatomy
brains
(thereafter:
"experimental
sections").
This
achieved
by
registering
digital
image
experimental
section
with
three-dimensional
(3D)
atlas
essentially
based
on
third
version
Allen
Mouse
Brain
Common
Coordinate
Framework
(CCF
v3),
retrieving
graphical
region
delineations
annotations
from
3D
atlas,
superimposing
information
onto
computer
screen.
By
doing
so,
helps
solving
long-standing
problem
faced
researchers
investigating
under
light
microscope-that
correctly
identifying
distinct
contained
within
sections.
Specifically,
provides
intuitive,
readily-available
microscopy
tool
enhance
researchers'
ability
identify
specific
Extensive
validation
studies
demonstrated
technology
performs
remarkably
well
accurately
delineating
are
large
and/or
located
dorsal
parts
brains,
independent
whether
were
imaged
fluorescence
or
bright-field
microscopy.
highly
efficient
way
minute
accurate
real-time.
Biomedical Optics Express,
Год журнала:
2019,
Номер
10(12), С. 6595 - 6595
Опубликована: Ноя. 27, 2019
Large
scale
simultaneous
recording
of
fast
patterns
neural
activity
remains
challenging.
Volumetric
imaging
modalities
such
as
scanning-beam
light-sheet
microscopy
(LSM)
and
wide-field
light-field
(WFLFM)
fall
short
the
goal
due
to
their
complex
calibration
procedure,
low
spatial
resolution,
or
high-photobleaching.
Here,
we
demonstrate
a
hybrid
(LSLFM)
modality
that
yields
high
resolution
with
simplified
alignment
plane
excitation
plane.
This
new
combines
selective
illumination
volumetric
imaging.
overcomes
current
limitations
LSM
WFLFM
implementations.
Compared
LSM,
LSLFM
captures
data
at
frame
rate
50×
lower
than
requires
no
dynamic
calibration.
WFLFM,
produces
moderate
improvements
in
resolutions,
10
times
improvement
contrast
when
fluorescent
beads,
3.2×
signal-to-noise
ratio
detection
live
zebrafish
expressing
genetically
encoded
calcium
sensor.
ILAR Journal,
Год журнала:
2020,
Номер
61(2-3), С. 110 - 138
Опубликована: Янв. 1, 2020
Abstract
We
provide
here
a
current
overview
of
marmoset
(Callithrix)
evolution,
hybridization,
species
biology,
basic/biomedical
research,
and
conservation
initiatives.
Composed
2
subgroups,
the
aurita
group
(C
C
flaviceps)
jacchus
geoffroyi,
jacchus,
kuhlii,
penicillata),
this
relatively
young
primate
radiation
is
endemic
to
Brazilian
Cerrado,
Caatinga,
Atlantic
Forest
biomes.
Significant
impacts
on
Callithrix
within
these
biomes
resulting
from
anthropogenic
activity
include
(1)
population
declines,
particularly
for
group;
(2)
widespread
geographic
displacement,
biological
invasions,
range
expansions
penicillata;
(3)
hybridization;
(4)
epizootic
Yellow
Fever
Zika
viral
outbreaks.
A
number
legal
initiatives
are
now
in
place
protect
threatened
increase
research
about
them.
Due
their
small
size
rapid
life
history,
marmosets
prized
biomedical
models.
As
result,
there
increasingly
sophisticated
genomic
resources
available
burgeoning
functional,
immuno-,
epigenomic
research.
In
both
laboratory
wild,
have
given
us
insight
into
cognition,
social
dynamics,
human
disease,
pregnancy.
penicillata
emerging
neotropical
models
arbovirus
including
Dengue
Zika.
Wild
populations
helping
understand
sylvatic
transmission
spillover
viruses.
All
factors
positioning
as
preeminent
facilitate
understanding
facets
conservation,
infectious
diseases.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2021,
Номер
unknown
Опубликована: Июнь 29, 2021
Abstract
The
sharing
of
research
data
is
essential
to
ensure
reproducibility
and
maximize
the
impact
public
investments
in
scientific
research.
Here
we
describe
OpenNeuro,
a
BRAIN
Initiative
archive
that
provides
ability
openly
share
from
broad
range
brain
imaging
types
following
FAIR
principles
for
sharing.
We
highlight
importance
Brain
Imaging
Data
Structure
(BIDS)
standard
enabling
effective
curation,
sharing,
reuse
data.
presently
shares
more
than
600
datasets
including
20,000
participants,
comprising
multiple
species
measurement
modalities
phenotypes.
shared
evident
growing
number
published
reuses,
currently
totalling
150
publications.
conclude
by
describing
plans
future
development
integration
with
other
ongoing
open
science
efforts.
3D
imaging
data
necessitate
reference
atlases
for
accurate
quantitative
interpretation.
Existing
computational
methods
to
generate
from
2D-derived
result
in
extensive
artifacts,
while
manual
curation
approaches
are
labor-intensive.
We
present
a
approach
atlas
construction
that
substantially
reduces
artifacts
by
identifying
anatomical
boundaries
the
underlying
and
using
these
guide
transformation.
Anatomical
also
allow
extension
of
complete
edge
regions.
Applying
eight
developmental
stages
Allen
Developing
Mouse
Brain
Atlas
(ADMBA)
led
more
comprehensive
atlases.
generated
15
whole
mouse
brains
validate
performance
observed
qualitative
improvement
(37%
greater
alignment
between
boundaries).
provide
pipeline
as
MagellanMapper
software
reconstructed
ADMBA
These
resources
facilitate
whole-organ
analysis
samples
across
development.
Brain stimulation,
Год журнала:
2021,
Номер
14(5), С. 1393 - 1403
Опубликована: Авг. 27, 2021
BackgroundNumerous
neuromodulatory
therapies
are
currently
under
investigation
or
in
clinical
use
for
the
treatment
of
psychiatric
conditions.Objective/hypothesisWe
sought
to
catalogue
past
and
present
human
research
studies
on
neuromodulation
identify
relevant
trends
this
field.MethodsClinicalTrials.gov
(https://www.clinicaltrials.gov/)
International
Clinical
Trials
Registry
Platform
(https://www.who.int/ictrp/en/)
were
queried
March
2020
trials
assessing
outcome
disorders.
Relevant
categorized
by
variables
such
as
modality,
country,
brain
target,
publication
status,
design,
funding
source.ResultsFrom
72,086
initial
search
results,
1252
unique
identified.
The
number
registered
annually
has
consistently
increased.
Half
all
active
a
quarter
have
translated
publications.
largest
proportion
involved
depression
(45%),
schizophrenia
(18%),
substance
disorders
(14%).
spanned
37
countries;
China,
second
contributor
(13%)
after
United
States
(28%),
increased
its
output
substantially
recent
years.
Over
75%
non-convulsive
non-invasive
modalities
(e.g.,
transcranial
magnetic
stimulation),
while
convulsive
electroconvulsive
therapy)
invasive
deep
stimulation)
less
represented.
72%
featured
approved
cleared
interventions.
Characteristic
inter-modality
differences
observed
with
respect
enrollment
size,
trial
design/phase,
funding.
Dorsolateral
prefrontal
cortex
accounted
over
half
focal
targets.
examining
biological
correlates
increased.Conclusion(s)These
results
provide
comprehensive
overview
state
research,
revealing
growing
scope
internationalism
field.