Lethal effects of high-intensity violet 405-nm light on Saccharomyces cerevisiae, Candida albicans, and on dormant and germinating spores of Aspergillus niger

Fungal Biology, Год журнала: 2013, Номер 117(7-8), С. 519 - 527

Опубликована: Май 30, 2013

Язык: Английский

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Light in the Fungal World: From Photoreception to Gene Transcription and Beyond

Annual Review of Genetics, Год журнала: 2019, Номер 53(1), С. 149 - 170

Опубликована: Авг. 27, 2019

Fungi see light of different colors by using photoreceptors such as the White Collar proteins and cryptochromes for blue light, opsins green phytochromes red light. Light regulates fungal development, promotes accumulation protective pigments proteins, tropic growth. The complex (WCC) is a photoreceptor transcription factor that responsible regulating after exposure to In Neurospora crassa, interaction WCCs their binding promoters activate transcription. Aspergillus nidulans, WCC phytochrome interact coordinate gene other responses, but contribution these photobiology varies across species. Ultimately, effect on biology result coordinated transcriptional regulation activation signal transduction pathways.

Язык: Английский

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Fungi use the SakA (HogA) pathway for phytochrome-dependent light signalling

Nature Microbiology, Год журнала: 2016, Номер 1(5)

Опубликована: Фев. 29, 2016

Язык: Английский

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Induction of sporulation in plant pathogenic fungi

Mycology&#58 An International Journal on Fungal Biology, Год журнала: 2012, Номер 3(3), С. 195 - 200

Опубликована: Авг. 23, 2012

Spore morphologies are a major character in fungal taxonomy, although many isolates not able to sporulate on common artificial media. This article reviews the effect of nutrition, host tissue, and light sporulation A trial experiment using 42 strains that failed potato dextrose agar (PDA) half-strength PDA after 3 months is reported. Five strategies (1/10-strength PDA, CaCO3 water agar, pine needle medium, mulberry near-ultraviolet irradiation) were applied induce these sporulate, with an overall success rate 62%. Pine medium was most successful method, which induced 40% recalcitrant strains.

Язык: Английский

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The VELVET Complex in the Gray Mold FungusBotrytis cinerea: Impact of BcLAE1 on Differentiation, Secondary Metabolism, and Virulence

Molecular Plant-Microbe Interactions, Год журнала: 2015, Номер 28(6), С. 659 - 674

Опубликована: Янв. 27, 2015

Botrytis cinerea, the gray mold fungus, is an important plant pathogen. Field populations are characterized by variability with regard to morphology, mode of reproduction (conidiation or sclerotia formation), spectrum secondary metabolites (SM), and virulence. Natural variation in bcvel1 encoding ortholog Aspergillus nidulans VeA, a member VELVET complex, was previously shown affect light-dependent differentiation, formation oxalic acid (OA), To gain broader insight into B. cinerea A. LaeA, BcLAE1, putative interaction partner BcVEL1, studied. BcVEL1 but not its truncated versions interacts BcLAE1 BcVEL2 (VelB ortholog). In accordance expected common as well specific functions deletions both genes result similar though identical phenotypes. Both mutants lost ability produce OA, colonize host tissue, form sclerotia. However, differ aerial hyphae conidia formation. Genome-wide expression analyses revealed that have distinct target genes. Some underexpressed mutants, e.g., those SM-related enzymes, proteases, carbohydrate-active may account for their reduced

Язык: Английский

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A Large-Scale Functional Analysis of Putative Target Genes of Mating-Type Loci Provides Insight into the Regulation of Sexual Development of the Cereal Pathogen Fusarium graminearum

PLoS Genetics, Год журнала: 2015, Номер 11(9), С. e1005486 - e1005486

Опубликована: Сен. 3, 2015

Fusarium graminearum, the causal agent of head blight in cereal crops, produces sexual progeny (ascospore) as an important overwintering and dissemination strategy for completing disease cycle. This homothallic ascomycetous species does not require a partner mating; instead, it carries two opposite mating-type (MAT) loci single nucleus to control development. To gain comprehensive understanding regulation development F. we used in-depth high-throughput analyses examine target genes controlled transcriptionally by two-linked MAT (MAT1-1, MAT1-2). We hybridized genome-wide microarray with total RNAs from graminearum mutants that lacked each locus individually or together, overexpressed MAT1-2-1, well their wild-type progenitor, at early stage A comparison gene expression levels revealed 1,245 differentially expressed (DEGs) among all examined. Among these, involved metabolism, cell wall organization, cellular response stimuli, adhesion, fertilization, development, chromatin silencing, signal transduction, were significantly enriched. Protein binding analysis presence putative core DNA sequences (ATTAAT ATTGTT) HMG (high mobility group)-box motif MAT1-2-1 protein. Targeted deletion 106 DEGs 25 specifically required most which regulated both MAT1-1 MAT1-2 loci. Taken together patterns key genes, propose regulatory pathway MAT-mediated may be activated several environmental cues via remodeling and/or signaling pathways, then least during cascades networks involving downstream transcription factors RNA interference pathway.

Язык: Английский

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The Histone Acetyltransferase GcnE (GCN5) Plays a Central Role in the Regulation ofAspergillusAsexual Development

Genetics, Год журнала: 2014, Номер 197(4), С. 1175 - 1189

Опубликована: Июнь 7, 2014

Abstract Acetylation of histones is a key regulatory mechanism gene expression in eukaryotes. GcnE an acetyltransferase Aspergillus nidulans involved the acetylation histone H3 at lysine 9 and 14. Previous works have demonstrated that deletion gcnE results defects primary secondary metabolism. Here we unveil role development show ∆gcnE mutant strain has minor growth but impaired normal conidiophore development. No signs conidiation were found after 3 days incubation, immature aberrant conidiophores 1 week incubation. Centroid linkage clustering principal component (PC) analysis transcriptomic data suggest occupies central position developmental regulation it essential for inducing genes. function was to be required H3K9/K14 promoter master regulator conidiation, brlA, as well promoters upstream regulators flbA, flbB, flbC, flbD (fluffy genes). However, brlA fluffy genes revealed lack originated complete absence strain. Ectopic induction from heterologous alcA did not remediate strain, suggesting additional GcnE-mediated mechanisms must operate. Therefore, conclude only nonessential modifier with strong fungal so far.

Язык: Английский

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Changes of global gene expression and secondary metabolite accumulation during light-dependent Aspergillus nidulans development

Fungal Genetics and Biology, Год журнала: 2016, Номер 87, С. 30 - 53

Опубликована: Янв. 7, 2016

Язык: Английский

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Red- and Blue-Light Sensing in the Plant Pathogen Alternaria alternata Depends on Phytochrome and the White-Collar Protein LreA

mBio, Год журнала: 2019, Номер 10(2)

Опубликована: Апрель 8, 2019

Light controls many processes in filamentous fungi. The study of light regulation a number model organisms revealed an unexpected complexity. Although the molecular components for sensing appear to be widely conserved fungal genomes, regulatory circuits and sensitivity certain species toward specific wavelengths seem different. In N. crassa , most responses are triggered by blue light, whereas A. nidulans red plays dominant role. Alternaria alternata both important. photoreceptors control morphogenetic pathways, homeostasis reactive oxygen species, production secondary metabolites. On other hand, high-osmolarity required FphA LreA, indicating sophisticated cross talk between stress signaling.

Язык: Английский

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Strategies for Controlling the Sporulation in Fusarium spp.

Journal of Fungi, Год журнала: 2022, Номер 9(1), С. 10 - 10

Опубликована: Дек. 21, 2022

Fusarium species are the most destructive phytopathogenic and toxin-producing fungi, causing serious diseases in almost all economically important plants. Sporulation is an essential part of life cycle Fusarium. frequently produces three different types asexual spores, i.e., macroconidia, chlamydospores, microconidia. It also meiotic but fewer than 20% Fusaria have a known sexual cycle. Therefore, spores play role their propagation infection. This review places special emphasis on current developments artificial anti-sporulation techniques as well features Fusarium's sporulation regulation, such temperature, light, pH, host tissue, nutrients. description regulation aspects strategies will help to shed light ways effectively control by inhibiting production which eventually improves food

Язык: Английский

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