Sensors and Actuators B Chemical, Год журнала: 2019, Номер 299, С. 126991 - 126991
Опубликована: Авг. 17, 2019
Язык: Английский
Sensors and Actuators B Chemical, Год журнала: 2019, Номер 299, С. 126991 - 126991
Опубликована: Авг. 17, 2019
Язык: Английский
eLife, Год журнала: 2021, Номер 10
Опубликована: Июнь 8, 2021
Intracellular density impacts the physical nature of cytoplasm and can globally affect cellular processes, yet regulation remains poorly understood. Here, using a new quantitative phase imaging method, we determined that dry-mass in fission yeast is maintained narrow distribution exhibits homeostatic behavior. However, varied during cell cycle, decreasing G2, increasing mitosis cytokinesis, dropping rapidly at birth. These variations were explained by constant rate biomass synthesis, coupled to slowdown volume growth division rapid expansion post-cytokinesis. Arrest specific cell-cycle stages exacerbated changes. Spatially heterogeneous patterns suggested links between regulation, tip growth, intracellular osmotic pressure. Our results demonstrate systematic cycle are predominantly due modulation expansion, reveal functional consequences gradients arrests.
Язык: Английский
Процитировано
55Applied Microbiology and Biotechnology, Год журнала: 2020, Номер 104(11), С. 4659 - 4674
Опубликована: Апрель 8, 2020
Язык: Английский
Процитировано
53Disease Models & Mechanisms, Год журнала: 2022, Номер 15(6)
Опубликована: Июнь 1, 2022
ABSTRACT For decades, budding yeast, a single-cellular eukaryote, has provided remarkable insights into human biology. Yeast and humans share several thousand genes despite morphological cellular differences over billion years of separate evolution. These encode critical processes, the failure which in results disease. Although recent developments genome engineering mammalian cells permit genetic assays cell lines, there is still need to develop biological reagents study disease variants high-throughput manner. Many protein-coding can successfully substitute for their yeast equivalents sustain growth, thus opening up doors developing direct gene function tractable system referred as ‘humanized yeast’. Humanized permits discovery new biology by measuring protein activity simplified organismal context. This Review summarizes showing how humanized directly assay explore variant effects at scale. Thus, extending ‘awesome power genetics’ biology, humanizing reinforces high relevance evolutionarily distant model organisms evolution,
Язык: Английский
Процитировано
33BMC Biology, Год журнала: 2016, Номер 14(1)
Опубликована: Июнь 22, 2016
Modern biomedical research depends critically on access to databases that house and disseminate genetic, genomic, molecular, cell biological knowledge. Even as the explosion of available genome sequences associated genome-scale data continues apace, sustainability professionally maintained is under threat due policy changes by major funding agencies. Here, we focus model organism demonstrate myriad ways in which not only act repositories but actively facilitate advances research. We present show reducing financial support could prove be just scientifically, also economically, unsound.
Язык: Английский
Процитировано
53Genome Research, Год журнала: 2017, Номер 27(10), С. 1685 - 1695
Опубликована: Сен. 15, 2017
Alternative polyadenylation (APA) is a widespread mechanism that generates mRNA isoforms with distinct properties. Here we have systematically mapped and compared cleavage sites (PASs) in two yeast species, S. cerevisiae pombe . Although >80% of the genes each species were found to display APA, showed greater 3′ UTR size differences among APA than did PASs different locations gene are surrounded sequences both often associated motifs involved Nrd1-Nab3-Sen1 termination pathway. In pombe, strong surrounding distal lead higher abundances long short ones, feature opposite Differences PAS placement between convergent starkly antisense transcript landscapes budding fission yeasts. more likely be expressed when cells growing nutrient-rich media, although groups affected species. Significantly, shortening coordinates up-regulation expression for translation during cell proliferation. Using strains deficient Pcf11 or Pab2, show reduced 3′-end processing factors lengthens UTR, having potent effect Pab2. Taken together, our data indicate mechanisms largely different: has many features Pab2 role regulation its mammalian homolog, PABPN1.
Язык: Английский
Процитировано
53Journal of Cell Science, Год журнала: 2016, Номер 129(22), С. 4289 - 4304
Опубликована: Окт. 14, 2016
Autophagy cargos include not only soluble cytosolic materials but also bulky organelles, such as ER and mitochondria. In budding yeast, two proteins that contain the PX domain BAR domain, Atg20 Atg24 (also known Snx42 Snx4, respectively) are required for organelle autophagy contribute to general in a way can be masked by compensatory mechanisms. It remains unclear why these important autophagy. Here, we show distantly related fungal organism, fission yeast Schizosaccharomyces pombe, of mitochondria is induced nitrogen starvation promoted three Atg20- Atg24-family - Atg20, SPBC1711.11 (named here Atg24b). These localize at pre-autophagosomal structure, or phagophore assembly site (PAS), during starvation. S. pombe forms homo-oligomer acts redundantly with Atg24b, latter form hetero-oligomer. The defect caused loss associated reduction autophagosome size decrease Atg8 accumulation PAS. results provide new insights into autophagic function proteins.
Язык: Английский
Процитировано
48Proceedings of the National Academy of Sciences, Год журнала: 2020, Номер 117(14), С. 7575 - 7583
Опубликована: Март 25, 2020
For cells to replicate, a sufficient supply of biosynthetic precursors is needed, necessitating the concerted action metabolism and protein synthesis during progressive phases cell division. A global understanding which processes are involved how they temporally regulated replication is, however, currently lacking. Here, quantitative multiomics analysis used generate holistic view eukaryal cycle, using budding yeast Saccharomyces cerevisiae Protein central carbon pathways such as glycolysis amino acid shown synchronize their respective abundance profiles with division, pathway-specific changes in metabolite also being reflected by relative increase mitochondrial volume, fluorescence microscopy. These results show precursor production be meet phase-specific demands
Язык: Английский
Процитировано
41FEBS Journal, Год журнала: 2021, Номер 289(1), С. 262 - 278
Опубликована: Июль 26, 2021
Mitochondria form a branched tubular network in many types of cells, depending on balance between mitochondrial fusion and fission. How fission are involved regulating function cell proliferation is not well understood. Here, we dissected the roles yeast. We examined membrane potential by staining cells with DiOC 6 assessed respiration directly measuring oxygen consumption dissolved respirometer. found that defects or reduce compromise while absence both restores wild type‐like respiration, normal potential, networks mitochondria. Moreover, either prolongs cycle significantly delays progression after nitrogen replenishment. The prolonged/delayed likely due to deregulation Cdc2 activation. Hence, our work only establishes an intimate link morphology but also underscores importance dynamics cycle.
Язык: Английский
Процитировано
36G3 Genes Genomes Genetics, Год журнала: 2023, Номер 13(4)
Опубликована: Фев. 7, 2023
Fission yeasts are an ancient group of fungal species that diverged from each other tens to hundreds million years ago. Among them is the preeminent model organism Schizosaccharomyces pombe, which has significantly contributed our understandings molecular mechanisms underlying fundamental cellular processes. The availability genomes S. pombe and 3 fission yeast japonicus, octosporus, cryophilus enabled cross-species comparisons provide insights into evolution genes, pathways, genomes. Here, we performed genome sequencing on type strain recently identified osmophilus obtained a complete mitochondrial nuclear assembly with gaps only at rRNA gene arrays. A total 5,098 protein-coding genes were annotated orthologs for more than 95% identified. Genome-based phylogenetic analysis showed most closely related octosporus these 2 around 16 To demonstrate utility this reference genome, conducted comparative analyses centromeres, telomeres, transposons, mating-type region, Cbp1 family proteins, These revealed conservation repeat arrangements sequence motifs in centromere cores, telomeric sequences composed types repeats, delineated relationships among Tf1/sushi retrotransposons, characterized evolutionary origins trajectories domesticated transposases, discovered signs interspecific transfer selfish elements.
Язык: Английский
Процитировано
16Journal of Fungi, Год журнала: 2023, Номер 9(5), С. 523 - 523
Опубликована: Апрель 28, 2023
Transcriptional regulation is vital for organismal survival, with many layers and mechanisms collaborating to balance gene expression. One layer of this genome organization, specifically the clustering functionally related, co-expressed genes along chromosomes. Spatial organization allows position effects stabilize RNA expression transcription, which can be advantageous a number reasons, including reductions in stochastic influences between products. The co-regulated families into functional clusters occurs extensively Ascomycota fungi. However, less characterized within related Basidiomycota fungi despite uses applications species clade. This review will provide insight prevalence, purpose, significance across Dikarya, foundational studies from Ascomycetes current state our understanding throughout representative Basidiomycete species.
Язык: Английский
Процитировано
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