Evaluating the effect of semen storage and dilution rate on boar sperm quality DOI
José Manuel León Jurado, Francisco Sevilla, Ignacio Araya‐Zúñiga

и другие.

Acta Agriculturae Scandinavica Section A – Animal Science, Год журнала: 2024, Номер unknown, С. 1 - 13

Опубликована: Ноя. 27, 2024

This study analyzed the effect of boar semen storage and dilution rate on sperm motility, swimming patterns kinematics. It used 35 sexually mature, healthy boars, with an average age 19.0 ± 10.34 months known fertility. Samples were separated into four treatments according to ratio (1:1, 1:4, 1:8 1:16; v:v) Androstar Plus® refrigerated at 17°C in dark for 96 h. Subsamples 12, 24, 28, 72 h using ISAS®v1 Computer-Assisted Semen Analysis System (CASA). Total progressive motility decreased (P < 0.05) as time increased. The percentage morphological abnormalities increased after 48 storage. diluted 1:1 (v:v) had higher values total faster spermatozoa compared other rates. was concluded that plasma membrane integrity showed a significant downward pattern

Язык: Английский

Efecto del diluyente, la edad y el estado sexual bovino sobre la cinemática del semen DOI Creative Commons
Ignacio Araya‐Zúñiga, Francisco Sevilla, Vinicio Barquero

и другие.

Agronomía Mesoamericana, Год журнала: 2023, Номер unknown, С. 52597 - 52597

Опубликована: Июль 12, 2023

Introducción. Los patrones cinemáticos del semen se condicionan por variación individual y/o un efecto ambiental determinado el manejo posterior a la obtención de muestra. Objetivo. Evaluar dos diluyentes comerciales, edad y estado sexual los toros sobre movilidad cinética espermática fresco bovino. Materiales métodos. El estudio realizó en las fincas ganaderas “La Vega”, San Carlos, Balsa”, Ramón, Costa Rica, entre marzo agosto 2021. Se utilizó proveniente trece Brahman, extraído electroeyaculación. utilizaron 45 eyaculados total, con mínimo máximo cinco animal. diluyó Optixcell® Bioxcell®, una temperatura 37 °C. Las muestras analizaron mediante Sistema Computarizado Análisis Seminal (Computer-Assisted Semen Analysis, CASA)-Mot ISAS®v1 cámara recuento reutilizable Spermtrack® 20 μm profundidad. Resultados. observaron diferencias (p<0,05) respecto cinemática espermatozoides, así como parámetros espemática, según diluyente utilizado. Con uso Bioxcell® hubo mayor progresiva (64,03±2,01 %) comparación Optixcell®. diluidos presentaron patrón cinemático más lineal progresivo que Optixcell®, valores menores velocidad promedio (VAP=106,67±0,24 s-1) e índice rectitud (STR=79,35±0,10 %). La no mostró para sin embargo, fue animales >48 meses (62,27±1,87 observó sexual, durante monta presentó linealidad, oscilación comparado al descanso sexual. Conclusión. edad, utilizado preservación condicionaron calidad bovinos.

Процитировано

3

Exploration of semen quality analyzed by casa-mot systems of brahman bulls infected with BLV and BHV-1 DOI Creative Commons
Derling Pichardo-Matamoros, Francisco Sevilla, Jorge Alberto Elizondo-Salazar

и другие.

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Окт. 31, 2023

Enzootic bovine leukosis virus (BLV) and herpesvirus 1 (BHV-1) are very important infectious agents for the livestock industry worldwide. The present study aimed to explore association between natural exposure BLV BHV-1 with sperm quality analyzed by Computer-Assisted Semen Analysis (CASA) systems. Ten sexually mature Brahman bulls, sanitary status BLV+/BHV-1+ (n = 2), BLV-/BHV-1+ 6) BLV-/BHV-1- 2) were evaluated twice, 30 days apart. Results showed that of each bull was not associated semen quality. It found from second collection better due interruption sexual rest. evidence thus revealed a infected generated good-quality contaminated and, therefore, it is essential detect seminal samples prevent spread BLV. A multivariate analysis presence four subpopulations in bulls differ significantly their kinematic patterns respect (P < 0.05), indicating infection-free seronegative best parameters, which improved discrimination according status. Overall, analyses indicate seropositive-infected should be excluded beef cattle farms.

Язык: Английский

Процитировано

2

Evaluating the effect of semen storage and dilution rate on boar sperm quality DOI
José Manuel León Jurado, Francisco Sevilla, Ignacio Araya‐Zúñiga

и другие.

Acta Agriculturae Scandinavica Section A – Animal Science, Год журнала: 2024, Номер unknown, С. 1 - 13

Опубликована: Ноя. 27, 2024

This study analyzed the effect of boar semen storage and dilution rate on sperm motility, swimming patterns kinematics. It used 35 sexually mature, healthy boars, with an average age 19.0 ± 10.34 months known fertility. Samples were separated into four treatments according to ratio (1:1, 1:4, 1:8 1:16; v:v) Androstar Plus® refrigerated at 17°C in dark for 96 h. Subsamples 12, 24, 28, 72 h using ISAS®v1 Computer-Assisted Semen Analysis System (CASA). Total progressive motility decreased (P < 0.05) as time increased. The percentage morphological abnormalities increased after 48 storage. diluted 1:1 (v:v) had higher values total faster spermatozoa compared other rates. was concluded that plasma membrane integrity showed a significant downward pattern

Язык: Английский

Процитировано

0