Efecto del diluyente, la edad y el estado sexual bovino sobre la cinemática del semen
Agronomía Mesoamericana,
Journal Year:
2023,
Volume and Issue:
unknown, P. 52597 - 52597
Published: July 12, 2023
Introducción.
Los
patrones
cinemáticos
del
semen
se
condicionan
por
variación
individual
y/o
un
efecto
ambiental
determinado
el
manejo
posterior
a
la
obtención
de
muestra.
Objetivo.
Evaluar
dos
diluyentes
comerciales,
edad
y
estado
sexual
los
toros
sobre
movilidad
cinética
espermática
fresco
bovino.
Materiales
métodos.
El
estudio
realizó
en
las
fincas
ganaderas
“La
Vega”,
San
Carlos,
Balsa”,
Ramón,
Costa
Rica,
entre
marzo
agosto
2021.
Se
utilizó
proveniente
trece
Brahman,
extraído
electroeyaculación.
utilizaron
45
eyaculados
total,
con
mínimo
máximo
cinco
animal.
diluyó
Optixcell®
Bioxcell®,
una
temperatura
37
°C.
Las
muestras
analizaron
mediante
Sistema
Computarizado
Análisis
Seminal
(Computer-Assisted
Semen
Analysis,
CASA)-Mot
ISAS®v1
cámara
recuento
reutilizable
Spermtrack®
20
μm
profundidad.
Resultados.
observaron
diferencias
(p<0,05)
respecto
cinemática
espermatozoides,
así
como
parámetros
espemática,
según
diluyente
utilizado.
Con
uso
Bioxcell®
hubo
mayor
progresiva
(64,03±2,01
%)
comparación
Optixcell®.
diluidos
presentaron
patrón
cinemático
más
lineal
progresivo
que
Optixcell®,
valores
menores
velocidad
promedio
(VAP=106,67±0,24
s-1)
e
índice
rectitud
(STR=79,35±0,10
%).
La
no
mostró
para
sin
embargo,
fue
animales
>48
meses
(62,27±1,87
observó
sexual,
durante
monta
presentó
linealidad,
oscilación
comparado
al
descanso
sexual.
Conclusión.
edad,
utilizado
preservación
condicionaron
calidad
bovinos.
Exploration of semen quality analyzed by casa-mot systems of brahman bulls infected with BLV and BHV-1
Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: Oct. 31, 2023
Enzootic
bovine
leukosis
virus
(BLV)
and
herpesvirus
1
(BHV-1)
are
very
important
infectious
agents
for
the
livestock
industry
worldwide.
The
present
study
aimed
to
explore
association
between
natural
exposure
BLV
BHV-1
with
sperm
quality
analyzed
by
Computer-Assisted
Semen
Analysis
(CASA)
systems.
Ten
sexually
mature
Brahman
bulls,
sanitary
status
BLV+/BHV-1+
(n
=
2),
BLV-/BHV-1+
6)
BLV-/BHV-1-
2)
were
evaluated
twice,
30
days
apart.
Results
showed
that
of
each
bull
was
not
associated
semen
quality.
It
found
from
second
collection
better
due
interruption
sexual
rest.
evidence
thus
revealed
a
infected
generated
good-quality
contaminated
and,
therefore,
it
is
essential
detect
seminal
samples
prevent
spread
BLV.
A
multivariate
analysis
presence
four
subpopulations
in
bulls
differ
significantly
their
kinematic
patterns
respect
(P
<
0.05),
indicating
infection-free
seronegative
best
parameters,
which
improved
discrimination
according
status.
Overall,
analyses
indicate
seropositive-infected
should
be
excluded
beef
cattle
farms.
Language: Английский
Evaluating the effect of semen storage and dilution rate on boar sperm quality
Acta Agriculturae Scandinavica Section A – Animal Science,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 13
Published: Nov. 27, 2024
This
study
analyzed
the
effect
of
boar
semen
storage
and
dilution
rate
on
sperm
motility,
swimming
patterns
kinematics.
It
used
35
sexually
mature,
healthy
boars,
with
an
average
age
19.0
±
10.34
months
known
fertility.
Samples
were
separated
into
four
treatments
according
to
ratio
(1:1,
1:4,
1:8
1:16;
v:v)
Androstar
Plus®
refrigerated
at
17°C
in
dark
for
96
h.
Subsamples
12,
24,
28,
72
h
using
ISAS®v1
Computer-Assisted
Semen
Analysis
System
(CASA).
Total
progressive
motility
decreased
(P
<
0.05)
as
time
increased.
The
percentage
morphological
abnormalities
increased
after
48
storage.
diluted
1:1
(v:v)
had
higher
values
total
faster
spermatozoa
compared
other
rates.
was
concluded
that
plasma
membrane
integrity
showed
a
significant
downward
pattern
Language: Английский