Investigating the human rhinovirus co-infection in patients with asthma exacerbations and COVID-19

Pharmacy Practice, Год журнала: 2022, Номер 20(2), С. 01 - 10

Опубликована: Июль 10, 2022

To investigate the co-infections with human rhinovirus (HRV) among patients asthma exacerbation and COVID-19 in Jordan. Also, to determine frequency of acute before during pandemic on a matched basis.The data this prospective cohort research consisted clinical variables. During first visit, after 14-days, nasopharyngeal swabs were taken quantitative polymerase chain reaction was performed for HRV SARS-CoV-2 detection.Forty-seven out 175 (26.9%) adult cases have been diagnosed asthma. The number exacerbations study participants higher 2021 than 2020 (p=0.035). Most included asthmatic (61.7%) only positive 38.3% co-infected HRV. cycle threshold value lower samples infected both viruses compared alone, p<0.005.Our findings indicate that significantly more prevalent stable Thus, and/or infections potentially cofactors or contributors cohort. This is study, Jordan, co-infection could be related severity COVID-19.

Язык: Английский

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Molecular Detection of Antibiotic Resistance Genes Using Respiratory Sample from Pneumonia Patients

Antibiotics, Год журнала: 2025, Номер 14(5), С. 502 - 502

Опубликована: Май 13, 2025

Antibiotic resistance makes the treatment of pneumonia challenging. Effective management depends on accurate diagnostic techniques to identify genes and customize drugs. This study primarily aimed antibiotic in respiratory samples from patients with using polymerase chain reaction (PCR) determine prevalence specific analyze clinical factors contributing resistance, as well provide actionable insights into patterns Jordan support efforts improve management. retrospective observational included 114 who were diagnosed pneumonia. Clinical data, including prior exposure history, collected. PCR diagnostics used detect samples. In this study, we evaluated 14 pathogens, highlighting their diverse mechanisms. Mec A was most frequently detected gene, appearing 87 (77.3%). Additionally, Tem 80 (70.2%), Oxa-48-like 15 (13.2%), Ctx-M-1 38 (33.3%) among commonly genes. contrast, Oxa-40-like (7.0%), Vim (8.8%), Imp (4.4%) exhibited a lower prevalence. The Oxa-51-like gene showed only significant association ertapenem (p-value = 0.046). Further analysis revealed statistically associations between methicillin (p < 0.001), underscoring its critical role. However, other genes, such Oxa-48-like, no correlation imipenem meropenem > 0.05). demonstrates utility PCR-based for detecting highlights associated These findings underscore importance integrating molecular routine care outcomes combat growing threat Jordan. PCR's value guiding effective strategies addressing multidrug-resistant

Язык: Английский

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Co‑infection of COVID-19 patients with atypical bacteria: A study based in Jordan

Pharmacy Practice, Год журнала: 2023, Номер 21(1), С. 01 - 05

Опубликована: Апрель 5, 2023

The aim of this work was to know the prevalence Chlamydophila pneumoniae and Mycoplasma in coronavirus disease 2019 (COVID-19) patients Jordan. Also, assess a TaqMan real-time polymerase chain reaction (PCR) assay detecting these two bacteria.This is retrospective study performed over last five months 2021. All nasopharyngeal specimens from COVID-19 were tested for C. pneumonia, M. pneumoniae. Pst-1 gene P1 cytadhesin protein targets.In study, 14 out 175 individuals with confirmed severe acute respiratory syndrome 2 (SARS-CoV-2) infection (8.0%) co-infected or Co-infection SARS-CoV-2 reported 5 (2.9%) patients, while 9 (5.1%) had co-infection. mean (± std) correlation coefficient calibration curve PCR analysis -0.993 0.001) -0.994 0.003) amplification efficiencies Pneumoniae 187.62% 136.86%, respectively.In first based Jordan, infected have low rate atypical bacterial However, clinicians should suspect co-infections both common uncommon bacteria patients. Large prospective investigations are needed give additional insight on true their impact clinical course

Язык: Английский

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Etiologies of Acute Bronchiolitis in Children at Risk for Asthma, with Emphasis on the Human Rhinovirus Genotyping Protocol

Journal of Clinical Medicine, Год журнала: 2023, Номер 12(12), С. 3909 - 3909

Опубликована: Июнь 8, 2023

This research aims to determine acute bronchiolitis’ causative virus(es) and establish a viable protocol classify the Human Rhinovirus (HRV) species. During 2021–2022, we included children 1–24 months of age with bronchiolitis at risk for asthma. The nasopharyngeal samples were taken subjected quantitative polymerase chain reaction (qPCR) in viral panel. For HRV-positive samples, high-throughput assay was applied, directing VP4/VP2 VP3/VP1 regions confirm BLAST searching, phylogenetic analysis, sequence divergence took place identify degree which these appropriate identifying differentiating HRV. HRV ranked second, following RSV, as etiology children. conclusion investigation all available data this study distributed sequences into 7 HRV-A, 1 HRV-B, HRV-C types based on sequences. nucleotide between clinical corresponding reference strains lower region than region. results demonstrated potential utility genotypes. Confirmatory outcomes yielded, indicating how nested semi-nested PCR can practical ways facilitate sequencing genotyping.

Язык: Английский

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Risk Factors for Pneumocystis jirovecii Pneumonia in Non-HIV Patients Hospitalized for COVID-19: A Case-Control Study

Journal of Fungi, Год журнала: 2023, Номер 9(8), С. 838 - 838

Опубликована: Авг. 11, 2023

Background: Very few cases of Pneumocystis jirovecii pneumonia (PJP) have been reported in COVID-19 so far, and mostly patients with concomitant HIV infection or solid-organ transplant recipients. Despite being associated lymphopenia the use steroids, there are no studies specifically aimed at investigating risk factors for PJP COVID-19. Methods: A retrospective case-control study was performed. We matched controls a 1:2 ratio, based on age ± 10 years, transplantation (SOT), hematological malignancies, setting development (ICU vs. non-ICU). direct immunofluorescence assay bronchoalveolar lavage fluid used to diagnose PJP. Results: enrolled 54 patients. Among 18 PJP, 16 were diagnosed as “proven”. Seven eighteen immunocompromised, while other had previous immunological impairment. Patients significantly lower median lymphocyte values (p = 0.033), longer duration 0.014), higher dose steroid received 0.026), CRP 0.005), SARS-CoV-2 vaccination rate than 0.029). Cumulative is independent factor (OR 1.004, 95%CI 1–1.008, p 0.042). Conclusions: develops regardless immunosuppressive conditions severity disease, it correlated corticosteroid received.

Язык: Английский

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Using laboratory parameters as predictors for the severity and mortality of COVID-19 in hospitalized patients

Pharmacy Practice, Год журнала: 2022, Номер 20(3), С. 01 - 09

Опубликована: Сен. 23, 2022

The aim of this study was to explore association initial laboratory parameters hospitalized patients with COVID-19, the severity and death incident.In retrospective study, were classified based on mortality outcome (survivor non-survivor) disease (non-severe, severe, critical). data (within first two days hospital admission) compared between these categories.Of 362 COVID-19 January-2021 May-2021, 39.0% non-severe, 32.2% 28.7% critical. 77.3% lived 22.7% died in hospital. Non-survivors significantly older than survivors. There a statistically significant exceeding cut-points or even death. These included D-dimer, C-reactive protein, prothrombin time, ferritin, white blood cells, neutrophil count, aspartate aminotransferase, creatinine, urea nitrogen, lymphocyte albumin. Also, showed high odds highest ratio reported for albumin <3.5 g/dL (OR=14.318 [4.784-42.851], p<0.001).The could effectively be used as predictors assess risk improve management patients.

Язык: Английский

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Development and Validation of a Diagnostic Nomogram for Pneumocystis jirovecii Pneumonia in Non-HIV-Infected Pneumonia Patients Undergoing Oral Glucocorticoid Treatment

Infection and Drug Resistance, Год журнала: 2023, Номер Volume 16, С. 755 - 767

Опубликована: Фев. 1, 2023

Pneumocystis jirovecii pneumonia (PJP) is an opportunistic but potentially fatal infection with increasing prevalence in HIV-free patients. Glucocorticoid therapy one of the most important risk factors for PJP. The delay diagnosis contributes to poor outcomes. Hence, aim this study was develop and validate a nomogram PJP patients non-HIV-infected who are undergoing oral glucocorticoid treatment.This retrospective, cross-sectional research. development group included 434 were admitted from 6 hospitals. Demographics, symptomatic features, laboratory computed tomography data analyzed using least absolute shrinkage selection operator (LASSO) select potential diagnostic indicators. Binary logistic regression used nomogram. Another 119 at Sichuan Provincial People's Hospital as validation group. performance measured by area under receiver-operating-characteristics curve (AUC), calibration curves, net benefit decision curve.PJP 25.3% LASSO revealed that age, lymphocyte count, fever, dry cough, respiratory failure, ground-glass opacity lungs, duration, immunosuppressive indicators showed robust discrimination, AUC 0.82 (95% CI 0.77-0.86) 0.87 0.80-0.94) group, both showing acceptable calibration. In analysis, our model consistently achieved greater across almost all ranges clinical thresholds.We developed good power receiving glucocorticoids. This may help promote timely treatment thus reduce mortality rate these

Язык: Английский

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Prognostic Factors Affecting Death in Patients with Rheumatoid Arthritis Complicated by Pneumocystis jirovecii Pneumonia and One-Year Clinical Course: The ANSWER Cohort Study

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(8), С. 7399 - 7399

Опубликована: Апрель 17, 2023

This multicenter retrospective study aimed to clarify the prognostic factors for mortality and changes in treatment modalities disease activities after onset of Pneumocystis jirovecii pneumonia (PCP) patients with rheumatoid arthritis (RA). Data regarding clinical background, modalities, activity indicators RA at PCP (baseline), 6 months 12 were extracted. Of 37 RA-PCP (median age, 69 years; 73% female), chemical prophylaxis was administered 8.1%. Six died during treatment. The serum C-reactive protein (CRP) levels prednisolone (PDN) dose baseline death group significantly higher than those survivor group. Multivariate analysis using a Cox regression model showed that PDN predictor from RA. During baseline, decreased. A high corticosteroids may result poor prognosis when is complicated. In future, preventive administration techniques must be established who need prevention.

Язык: Английский

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The first study characterizing the respiratory microbiome in cystic fibrosis patients in Jordan

Pharmacy Practice, Год журнала: 2023, Номер 21(3), С. 01 - 06

Опубликована: Окт. 5, 2023

Cystic fibrosis (CF) is most commonly seen in Caucasians and uncommon the Middle East. This study, based Jordan, aimed to describe association between lung exacerbation CF patients respiratory microbiome composition. Using 16S rRNA marker-gene sequencing, we investigated microbiota sputa during (E1) 14 days after (E2) of two admitted hospital. Detected genera with high abundance E1-related first patient included Achromobacter Streptococcus. At E2, Staphylococcus were highest abundant genera. Regarding second patient, Veillonella Streptococcus, at E1. Whereas, Streptococcus report patients. study suggests that pulmonary can result alterations their microbiome. A better knowledge this link could allow more focused use antibiotics, especially exacerbations, improving clinical efficacy outcomes

Язык: Английский

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Risk Factors for <em>Pneumocystis jirovecii </em>Pneumonia in Non-HIV Patients Hospitalized for COVID-19: A Case-Control Study

Опубликована: Авг. 7, 2023

Background Very few cases of Pneumocystis jirovecii pneumonia (PCP) have been reported in COVID-19 so far, mostly patients with concomitant HIV infection or solid organ transplant recipients. Despite is associated lymphopenia and use steroids, there are no studies specifically aimed to investigate risk factors for PCP Methods retrospective case-control study a cohort confirmed COVID-19. Direct immunofluorescence assay on respiratory samples was used diagnose PCP. Results We enrolled 54 patients. Patients had significant lower median lymphocyte values (p=0.033), longer disease duration (p=0.014), higher cumulative dose steroid received (p=0.026), CRP (p=0.005) SARS-CoV-2 vaccination rate than controls (p=0.029). Cumulative (p=0.042) the highest value during stay (p=0.012) were identified as factor PCP, while one (p=0.029) two doses (p=0.049) protective although not independently associated. Conclusions develops regardless immunosuppressive conditions. However, significantly steroidal dose, high lack vaccination.

Язык: Английский

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