Advances and Challenges of Bioassembly Strategies in Neurovascular In Vitro Modeling: An Overview of Current Technologies with a Focus on Three-Dimensional Bioprinting

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11000 - 11000

Опубликована: Окт. 12, 2024

Bioassembly encompasses various techniques such as bioprinting, microfluidics, organoids, and self-assembly, enabling advances in tissue engineering regenerative medicine. Advancements bioassembly technologies have enabled the precise arrangement integration of cell types to more closely mimic complexity functionality neurovascular unit (NVU) that other biodiverse multicellular structures. In this context, bioprinting offers ability deposit cells a spatially controlled manner, facilitating construction interconnected networks. Scaffold-based assembly strategies provide structural support guidance cues for growth, formation complex bio-constructs. Self-assembly approaches utilize inherent properties drive spontaneous organization interaction neuronal vascular components. However, recreating intricate microarchitecture functional characteristics tissue/organ poses additional challenges. materials hold great promise addressing these The further refinement technologies, improved resolution incorporation multiple types, can enhance accuracy biological constructs; however, developing bioinks growth cells, viability, while maintaining compatibility with process remains an unmet need field, advancements design bioactive biodegradable scaffolds will aid controlling adhesion, differentiation, vascularization within engineered tissue. Additionally, integrating advanced imaging analytical real-time monitoring characterization bioassembly, aiding quality control optimization. While challenges remain, ongoing research technological propel field forward, paving way transformative developments engineering. This work provides overview advancements, challenges, future perspectives fabricating constructs add-on focus on technologies.

Язык: Английский

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Regeneration of the female reproductive tract using 3D bioprinting technology

Virtual and Physical Prototyping, Год журнала: 2024, Номер 19(1)

Опубликована: Ноя. 14, 2024

Diseases of the female reproductive tract can arise from various congenital or acquired causes and may lead to loss tissue, functions, fertility. Current treatments do not offer permanent solutions, underscoring need for alternative therapeutic approaches. Tissue engineering regenerative medicine have emerged as promising strategies regenerating tissues restoring 3D bioprinting technology, in particular, has exhibited considerable potential treating tract. This review aims cover current tissue applications diseases highlight innovative solutions offered by technology uterus, ovary, cervix, vagina. By assessing significant studies advancements biomaterials cell sources, this emphasises great enhancing health

Язык: Английский

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Advances of Sulfonated Hyaluronic Acid in Biomaterials and Coatings—A Review

Coatings, Год журнала: 2023, Номер 13(8), С. 1345 - 1345

Опубликована: Июль 31, 2023

Hyaluronic acid (HA) is a non-sulfated glycosaminoglycan (GAG) that versatile material whose biological, chemical, and physical characteristics can be deeply tuned to modifications. However, HA easy decompose by hyaluronidase in vivo, this process will reduce its structure function stability during application. The sulfonation of improve under the action hyaluronidase. Sulfated hyaluronic (S-HA) synthesized many methods, it shows significantly slower degradation compared with HA. In addition, negatively charged S-HA has other advantages such as anti-adhesive activity, anti-inflammatory, macromolecules electrostatic interactions, stable site absorption positively molecules, enhancement growth factor binding ability. It numerous applications medical (anti-aging, inflammation, tissue regeneration, cancer therapy, wound healing, drug delivery) cosmetics biomaterials coatings. article, advances for potential application biomedical coatings reviewed comprehensively discussed.

Язык: Английский

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Molecular subtypes of ischemic heart disease based on circadian rhythm

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 19, 2024

Abstract Coronary atherosclerotic heart disease (CAD) is among the most prevalent chronic diseases globally. Circadian rhythm disruption (CRD) closely associated with progression of various diseases. However, precise role CRD in development CAD remains to be elucidated. The score (CRDscore) was employed quantitatively assess level samples. Our investigation revealed a significant association between high CRDscore and adverse prognosis patients, along substantial correlation progression. Remarkably distinct distributions were also identified subtypes. In summary, we have pioneered revelation relationship at single-cell established reliable markers for development, treatment, CAD. A deeper understanding these mechanisms may offer new possibilities incorporating "the therapy coronary based circadian rhythm" into personalized medical treatment regimens.

Язык: Английский

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Multi‐Organ Microphysiological Systems Targeting Specific Organs for Recapitulating Disease Phenotypes via Organ Crosstalk

Small Science, Год журнала: 2024, Номер 4(11)

Опубликована: Сен. 19, 2024

Various systemic metabolic diseases arise from prolonged crosstalk across multiple organs, triggering serious impairments in various physiological systems. These are intricate pathologies characterized by complex mechanisms and an unclear etiology, making the treatment challenging. Efforts have been made to develop vitro models understand these devise new treatments. However, there limitations reconstructing causal relationships between interorgan crosstalk, including tissue-specific microenvironment. Alternatively, multi-organ microphysiological systems (MOMPS) present possibilities for capturing complexity of replicating human microphysiology simulating diverse crosstalk. Controlled interactions scalable representations biological MOMPS offer a more accurate portrayal organ interactions, enabling identification novel metabolism, immunity. This, turn, can yield valuable insights into disease drug development research enhance efficiency preclinical studies. In this review, examples technical capabilities pathological modeling discussed, leveraging state-of-the-art biofabrication technology MOMPS. It evaluates current opportunities challenges field.

Язык: Английский

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Bioengineered Human Arterial Equivalent and Its Applications from Vascular Graft to In Vitro Disease Modeling

iScience, Год журнала: 2024, Номер 27(11), С. 111215 - 111215

Опубликована: Окт. 19, 2024

Язык: Английский

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Human acellular amniotic membrane/polycaprolactone vascular grafts prepared by electrospinning enable vascular remodeling in vivo

BioMedical Engineering OnLine, Год журнала: 2024, Номер 23(1)

Опубликована: Ноя. 6, 2024

Vascular transplantation is an effective treatment for severe vascular lesions. The design of the bioactive and mechanical properties small-caliber grafts critical their application in tissue engineering. In this study, we sought to develope a graft by electrospinning mixture human acellular amniotic membrane (HAAM) polycaprolactone (PCL). Mechanical tests showed that were strong enough endure stress from adjacent blood vessels pressure. biocompatibility HAAM/PCL was evaluated based on cell proliferation vitro. tubular formation test demonstrated containing HAAM could improve umbilical vein endothelial function, vivo implantation performed replacing rat abdominal aorta. found promote attachment retention. regenerated smooth muscle layer similar native arteries' endothelium coverage complete. These results suggest our constructs may be promising candidates can potentially used develop endothelialize rapidly vivo.

Язык: Английский

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Research Progress on the Application of Natural Medicines in Biomaterial Coatings

Materials, Год журнала: 2024, Номер 17(22), С. 5607 - 5607

Опубликована: Ноя. 16, 2024

With the continuous progress of biomedical technology, biomaterial coatings play an important role in improving performance medical devices and promoting tissue repair regeneration. The application natural medicine to biological materials has become a hot topic due its diverse activity, low toxicity, wide range sources. This article introduces definition classification medicines, lists some common such as curcumin, allicin, chitosan, tea polyphenols, etc., activities antibacterial, antioxidant, antitumor, other properties. According different characteristics physical adsorption, chemical grafting, layer-by-layer self-assembly, sol-gel methods are combined with biomaterials, which can be used for orthopedic implants, cardiovascular cerebrovascular stents, wound dressings, drug delivery systems, exert their activity. For example, antibacterial properties, regeneration, biocompatibility promote development health. Although biomaterials been greatly expanded, it still faces major challenges, whether combination between coating substrate is firm, load released sustainably, dynamic balance will disrupted, so on; series problems affects drugs coatings. In view these problems, this paper summarizes suggestions by evaluating literature, optimizing binding method release system; carrying out more clinical research; multidisciplinary cooperation; broadening coatings; developing safer, effective multi-functional through research innovation, contribute field.

Язык: Английский

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Biomimetic tubular materials: from native tissues to a unifying view of new vascular, tracheal, gastrointestinal, oesophageal, and urinary grafts

Chemical Society Reviews, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 28, 2024

We propose a simplified two-layered model of native tubular tissues, based on the composition and structure. This approach offers guidelines to elaborate biomimetic materials in field tissue engineering for clinical applications.

Язык: Английский

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Modelling, design, fabrication and characterization of engineered human myocardium made with melt electrowriting and cardiac cells derived from hiPSCs

Опубликована: Янв. 1, 2023

The adult human heart has evolved to become a highly specialized organ, whose continuous pumping of blood is critical for survival. However, its ability regenerate or self-repair following injury very limited, so consequently any event disease resulting in damage the poses serious threat patient. Moreover, cardiovascular diseases represent one most pressing healthcare concerns nowadays, as they are leading cause death worldwide, and number cases only expected increase years. Despite great progress made over years treat diseases, date there no therapy able fully cure that been damaged. In consequence, dire need generate new strategies repair restore lost cardiac function, well develop accurate modelling platforms advance understanding progression assess effectiveness drugs. Since advent, tissue engineering regenerative medicine regarded promising candidate realise this enormous challenge. Given interdisciplinary nature, scientific breakthroughs different areas such cellular reprogramming, polymer chemistry, additive manufacturing technologies have resulted advancement One cornerstone discoveries was generation induced pluripotent stem cells subsequent differentiation phenotypes, present Thesis revolves around their application patient-specific models humanised engineered functional minitissues. Firstly, we reprogrammed peripheral mononuclear from transthyretin amyloid cardiomyopathy patient, cell line carrying c.128G>A (p.Ser43Asn) mutation gene. Experiments demonstrated efficacy safety approach, confirming pluripotency cells, presence disease-causing mutation, removal reprogramming vectors. This line, which now available repository, can be used investigate biology, molecular mechanisms progression; an advanced model test novel therapeutic strategies. Secondly, aimed minitissues by combining cardiomyocytes derived tridimensional fibrillar scaffolds generated with technology melt electrowriting. Compared conventional two-dimensional culture, enhanced maturation, significant conduction velocity, connexin 43 expression cardiac-associated genes MYL2, GJA5 SCN5A, isoform ratios MYH7/MYH6 MYL2/MYL7 after 28 days culture. When investigating effect scaffold fibres on found placed close fibre were arranged parallel it, but alignment progressively towards centre pore. We then these data simulations capable accurately reproducing experimental performance. In-depth gauging structural disposition intercellular connectivity allowed us improved computational predict relationship between study lays down path advancing development silico tools biofabricated evolution generation, maps route more biomimetic manufacture. next at increasing biological representativity minitissues, implementing few changes (addition cell-derived fibroblasts) hydrogel (substitution Matrigel fibrin) composition. also sought control cardiomyocyte behaviour based electrowritten geometry. For this, hypothesized diamond-based would induce contraction direction least mechanical resistance, i.e., small diagonal diamonds. characterization maturation consistent previous work terms gene although observed low initial retention within highlighted improve seeding efficiency. comparing contractile dynamics square, rectangular, diamond-shaped pores, latter significantly faster, stronger aligned had anticipated. potential use agents tested implanting constructs murine chronic myocardial infarction. controls, implanted animals showed improvement, including higher left ventricular ejection fraction greater wall thickness. Finally, another attempt enhance constructs, proof concept ellipsoidal controlled pore architecture. summary, electrowriting efforts. By both iteratively optimising design conditions, increased relevance.

Язык: Английский

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