In Vitro Cellular & Developmental Biology - Animal,
Год журнала:
2021,
Номер
57(8), С. 763 - 774
Опубликована: Сен. 1, 2021
Diabetic
kidney
disease
(DKD)
has
become
the
most
common
cause
of
chronic
disease.
Proteinuria
is
generally
considered
one
clinical
indicators
renal
damage,
and
it
also
closely
related
to
progression
DKD.
Accumulating
evidence
indicates
that
proteinuria
induces
an
upregulation
expression
levels
inflammatory
cytokines
fibrosis
markers
in
tubular
epithelial
cells,
but
mechanism
remains
unclear.
Previously,
we
showed
early
growth
response
1
(Egr1)
played
a
key
role
injury.
However,
upstream
Egr1
development
DKD
poorly
understood.
In
this
study,
found
albumin
stimulation
significantly
increased
Egr1,
interleukin
6
(IL-6),
tumor
necrosis
factor-α
(TNF-α),
fibronectin
(FN)
HK-2
cells
decreased
miR-23a-3p
levels.
We
then
identified
targeted
3'
untranslated
region
(UTR)
directly
suppressed
Egr1.
Moreover,
overexpression
inhibition
attenuated
promoted
IL-6,
TNF-α,
FN,
respectively.
Additionally,
silencing
reversed
inflammation
caused
by
inhibitor.
Thus,
conclude
attenuates
through
suggesting
targeting
may
be
novel
therapeutic
approach
for
Molecular Medicine Reports,
Год журнала:
2020,
Номер
unknown
Опубликована: Сен. 2, 2020
Circular
RNAs
(circRNAs)
have
crucial
roles
in
various
diseases;
however,
the
mechanisms
of
action
underlying
circRNAs
occurrence
and
development
diabetic
nephropathy
(DN)
remains
largely
unknown.
The
present
study
investigated
differentially
expressed
DN
mice
kidney
cortex
using
circRNA
sequencing
elucidated
role
mesangial
cells.
It
was
revealed
that
40
were
unconventionally
expressed,
including
18
upregulated
22
downregulated
circRNAs.
Furthermore,
circ_0000491
levels
significantly
augmented
both
high
glucose
(HG,
30
mM)‑induced
mouse
cells
(MES13
cells).
Knockdown
suppressed
increase
vimentin,
fibronectin
α‑smooth
muscle
actin,
as
well
collagen
type
I,
III
IV,
whilst
reversing
decrease
E‑cadherin
HG‑induced
MES13
further
circRNA_0000491
sponged
miR‑101b
directly
targets
TGFβRI.
In
addition,
expression
negatively
associated
with
transcriptional
level
inhibitors
reversed
suppression
extracellular
matrix
(ECM)‑associated
protein
synthesis
mediated
by
knocking‑down
circRNA_0000491.
conclusion,
circRNA_0000491/miR‑101b/TGFβRI
axis
ECM
accumulation
fibrosis‑associated
cells,
which
suggested
may
be
beneficial
for
an
effective
therapeutic
target
DN.
Journal of Cellular and Molecular Medicine,
Год журнала:
2020,
Номер
24(22), С. 13314 - 13323
Опубликована: Окт. 3, 2020
Diabetic
nephropathy
(DN)
is
a
serious
kidney
disease
resulted
from
diabetes.
Dys-regulated
proliferation
and
extracellular
matrix
(ECM)
accumulation
in
mesangial
cells
contribute
to
DN
progression.
In
this
study,
we
tested
expression
level
of
MIAT
patients
treated
by
high
glucose
(HG).
Up-regulation
was
observed
DN.
Then,
functional
assays
displayed
that
silence
siRNA
significantly
repressed
the
cycle
progression
induced
HG.
Meanwhile,
found
collagen
IV,
fibronectin
TGF-β1
protein
obviously
triggered
HG,
which
could
be
rescued
loss
MIAT.
further
assessment
indicated
served
as
sponge
harbouring
miR-147a.
Moreover,
miR-147a
decreased
DN,
exhibited
an
antagonistic
effect
on
modulating
cell
fibrosis.
bioinformatics
analysis
E2F
transcription
factor
3
(E2F3)
act
direct
target
We
demonstrated
E2F3
greatly
increased
binding
association
between
evidenced
using
luciferase
reporter
assay.
summary,
our
data
explored
underlying
mechanism
pathogenesis
validated
fibrosis
via
sponging
regulating
E2F3.
Zinc
(Zn)
and
magnesium
(Mg)
are
essential
trace
elements
in
humans.
Their
deficiency
may
be
associated
with
inflammation
oxidative
stress
(OS)
patients
diabetic
nephropathy
(DN),
but
the
mechanisms
involved
have
not
been
fully
characterized.
We
aimed
to
investigate
relationships
between
circulating
concentrations
of
Zn
Mg
pro-inflammatory
factors
DN-associated
renal
functional
damage
type
2
diabetes
mellitus
(T2DM).
To
this
end,
we
studied
20
healthy
people,
24
T2DM,
59
T2DM
T2DN.
Serum
urine
were
measured
using
2-(5-nitro-2-pyridylazo)-5-(N-propyl-N-sulfopropylamine)
phenol
(nitro-PAPS)
chromogenic
method
xylidyl
blue
method,
respectively,
cytokines
[interleukin
(IL)-1β,
IL-6,
IL-8,
IL-10,
IL-12,
tumor
necrosis
factor-α
(TNF-α)]
flow
cytometry.
The
serum
significantly
lower
DN
than
controls.
Zn,
decreased,
while
those
IL-6
IL-8
increased
progression
damage.
Furthermore,
negatively
correlated
concentrations.
Notably,
concentration
was
found
independently
protect
against
T2DM.
Hypozincemia
T2DN-associated
because
it
exacerbates
inflammation.
In Vitro Cellular & Developmental Biology - Animal,
Год журнала:
2021,
Номер
57(8), С. 763 - 774
Опубликована: Сен. 1, 2021
Diabetic
kidney
disease
(DKD)
has
become
the
most
common
cause
of
chronic
disease.
Proteinuria
is
generally
considered
one
clinical
indicators
renal
damage,
and
it
also
closely
related
to
progression
DKD.
Accumulating
evidence
indicates
that
proteinuria
induces
an
upregulation
expression
levels
inflammatory
cytokines
fibrosis
markers
in
tubular
epithelial
cells,
but
mechanism
remains
unclear.
Previously,
we
showed
early
growth
response
1
(Egr1)
played
a
key
role
injury.
However,
upstream
Egr1
development
DKD
poorly
understood.
In
this
study,
found
albumin
stimulation
significantly
increased
Egr1,
interleukin
6
(IL-6),
tumor
necrosis
factor-α
(TNF-α),
fibronectin
(FN)
HK-2
cells
decreased
miR-23a-3p
levels.
We
then
identified
targeted
3'
untranslated
region
(UTR)
directly
suppressed
Egr1.
Moreover,
overexpression
inhibition
attenuated
promoted
IL-6,
TNF-α,
FN,
respectively.
Additionally,
silencing
reversed
inflammation
caused
by
inhibitor.
Thus,
conclude
attenuates
through
suggesting
targeting
may
be
novel
therapeutic
approach
for
