Scutellarin Acts via MAPKs Pathway to Promote M2 Polarization of Microglial Cells

Molecular Neurobiology, Год журнала: 2023, Номер 60(8), С. 4304 - 4323

Опубликована: Апрель 22, 2023

Язык: Английский

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Elevated microRNA-135b-5p relieves neuronal injury and inflammation in post-stroke cognitive impairment by targeting NR3C2

International Journal of Neuroscience, Год журнала: 2020, Номер 132(1), С. 58 - 66

Опубликована: Июль 26, 2020

Post-stroke cognitive impairment (PSCI) is a series of syndromes that meets the diagnostic criteria within 6 months after clinical event stroke. With unpleasing treatment at present, this study aimed to investigate role microRNA (miR)-135b-5p in regulating mineralocorticoid receptor (NR3C2) PSCI.The rats were modeled via middle cerebral artery occlusion, and injected with miR-135b-5p agomir or antagomir figure its post-stroke neurological deficits, neuronal injury, cell apoptosis, inflammation Behavioral tests, Nissl's staining, flow cytometry, TUNEL staining. The expression NR3C2 was detected by RT-qPCR western blot analysis. targeting relationship between verified dual luciferase reporter gene assay.Highly expressed relieved focal ischemia-reperfusion (FCIR) neuron reduced apoptosis inflammatory response FCIR PSCI rats. Poorly highly present injury can direct target 3'UTR.The highlights up regulation reduce NR3C2, which might be helpful for treatment.

Язык: Английский

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Ginsenoside Rg3 Prevents Oncogenic Long Noncoding RNA ATXN8OS from Inhibiting Tumor-Suppressive microRNA-424-5p in Breast Cancer Cells

Biomolecules, Год журнала: 2021, Номер 11(1), С. 118 - 118

Опубликована: Янв. 18, 2021

Ginsenoside Rg3 exerts antiproliferation activity on cancer cells by regulating diverse noncoding RNAs. However, little is known about the role of long RNAs (lncRNAs) or their relationship with competitive endogenous RNA (ceRNA) in Rg3-treated cells. Here, a lncRNA (ATXN8OS) was found to be downregulated via Rg3-mediated promoter hypermethylation MCF-7 breast SiRNA-induced downregulation ATXN8OS decreased cell proliferation but increased apoptosis, suggesting that possessed proproliferation activity. An silico search for potential ATXN8OS-targeting microRNAs (miRs) identified promising candidate (miR-424-5p) based its high binding score. As expected, miR-424-5p suppressed and stimulated apoptosis The miR-target-gene prediction 200 target genes miR-424-5p, which were subsequently narrowed down seven underwent at Rg3. Among them, three (EYA1, DACH1, CHRM3) previously oncogenes proven oppositely regulated miR-424-5p. When taken together, inhibited tumor-suppressive leading oncogenic genes.

Язык: Английский

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Long Non-Coding RNA-Mediated Competing Endogenous RNA Networks in Ischemic Stroke: Molecular Mechanisms, Therapeutic Implications, and Challenges

Frontiers in Pharmacology, Год журнала: 2021, Номер 12

Опубликована: Ноя. 17, 2021

Ischemic stroke (IS) is a disease that characterized by high mortality and disability. Recent studies have shown LncRNA-mediated competing endogenous RNA (ceRNA) networks play roles in the occurrence development of cerebral I/R injury regulating different signaling pathways. However, no systematic analysis ceRNA mechanisms IS has been reported. In this review, we discuss molecular under injury. The expression levels LncRNAs, microRNAs (miRNAs), messenger RNAs (mRNAs) their effects four major cell types neurovascular unit (NVU) are also involved. We further summarize LncRNAs as biomarkers therapeutic targets. Finally, analyze advantages limitations using therapeutics for IS.

Язык: Английский

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Research progress on the mechanism of action and clinical application of remote ischemic post-conditioning for acute ischemic stroke

Clinical Neurology and Neurosurgery, Год журнала: 2024, Номер 244, С. 108397 - 108397

Опубликована: Июнь 23, 2024

Язык: Английский

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Circulating circular RNAs as novel biomarkers and functional prediction for the early diagnosis in post-stroke cognitive impairment: A single-center prospective study in China

Journal of Stroke and Cerebrovascular Diseases, Год журнала: 2024, Номер 33(11), С. 107945 - 107945

Опубликована: Авг. 29, 2024

Язык: Английский

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Advances in the detection of biomarkers for ischemic stroke

Frontiers in Neurology, Год журнала: 2025, Номер 16

Опубликована: Фев. 24, 2025

Ischemic stroke is a leading cause of mortality and morbidity globally. Prompt intervention essential for arresting disease progression minimizing central nervous system damage. Although imaging studies play significant role in diagnosing ischemic stroke, their high costs limited sensitivity often result diagnostic treatment delays. Blood biomarkers have shown considerable promise the diagnosis prognosis stroke. Serum markers, closely associated with pathophysiology, aid diagnosis, subtype identification, prediction progression, early neurological deterioration, recurrence. Their advantages are particularly pronounced due to low cost rapid results. Despite identification numerous candidate blood biomarkers, clinical application requires rigorous research thorough validation. This review focuses on various related including coagulation fibrinolysis-related factors, endothelial dysfunction inflammatory neuronal axonal injury exosomes circular RNAs other relevant molecules. It also summarizes latest methods techniques biomarker detection, aiming provide critical references key biomarkers.

Язык: Английский

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LncRNAs Orchestrating Neuroinflammation: A Comprehensive Review

Cellular and Molecular Neurobiology, Год журнала: 2025, Номер 45(1)

Опубликована: Март 8, 2025

CNS diseases account for a major part of the comorbidity and mortality human population; moreover, neuroinflammation has become an indication different diseases, instance, Parkinson's Alzheimer's disease. Microglia astrocytes are two main glial cells that can be found in CNS. Each these plays important role mediating immune responses like inflammation. There many studies suggesting LncRNAs neuroinflammation. Indeed, orchestrate through various mechanisms, namely miRNA sponge, transcriptional activation/inhibition. In addition, regulate downstream pathways NF-κB, PI3K/AKT. this study, we gathered existing regarding mechanisms action pathogenesis neurodegenerative traumatic injuries regulating We aim to elaborate on regulatory roles bring more profound understanding etiology terms

Язык: Английский

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Long noncoding RNA MALAT1 promotes angiogenesis through the caveolin-1/VEGF pathway after cerebral ischemic injury

Neuroreport, Год журнала: 2025, Номер 36(7), С. 350 - 363

Опубликована: Апрель 9, 2025

The long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) might protect against cerebral ischemic injury. This study explored MALAT1’s function in stroke and whether it acts through the caveolin-1/vascular endothelial growth factor (VEGF) pathway. A mouse model of middle artery occlusion/reperfusion (MCAO/R) a human brain microvascular cell (HBMEC) oxygen-glucose deprivation/reoxygenation (OGD/R) were established. Lentiviral vectors for MALAT1 knockdown, caveolin-1 overexpression used gene regulation studies. Neurological deficits, proliferation, apoptosis, viability, vitro angiogenesis, migration, expression related protein evaluated using Zea Longa five-point scale, VEGF receptor 2/CD34 double immunofluorescence, TdT-mediated dUTP nick end labeling staining, counting kit-8 assay, tube formation transwell quantitative real time PCR, western blot. In MCAO/R HBMEC OGD/R model, levels MALAT1, caveolin-1, significantly upregulated compared to control group. vivo, downregulation exacerbated injury as manifested by severe neurological larger infarct volume, increased decreased numbers 2+/CD34+ progenitor cells, VEGF. Conversely, partially reversed inhibition migration tubule downregulation, restored promotes angiogenesis after injury, likely part via caveolin-1/VEGF Thus, may serve potential therapeutic target stroke.

Язык: Английский

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Decoding immune cell dynamics in ischemic stroke: insights from single-cell RNA sequencing analysis

Frontiers in Aging Neuroscience, Год журнала: 2025, Номер 17

Опубликована: Апрель 15, 2025

Background Ischemic stroke (IS) is a leading cause of adult disability worldwide. The inflammatory processes involved are complex, making it challenging to fully understand the pathological mechanisms IS. Phagocytosis plays an important role in eliminating neurotoxic or damaged neurons resulting from responses. This study employed bioinformatics methods analyze single-cell RNA sequencing (scRNA-seq) data investigate cell types and molecular biological Methods scRNA-seq for IS were obtained Gene Expression Omnibus (GEO). Following sample screening reprocessing, 5,582 single cells identified healthy controls patients with Uniform manifold approximation projection (UMAP) was utilized further explore cellular composition Functional enrichment analysis differentially expressed genes conducted identify transcriptional regulators, whereas developmental trajectories predicted uncover potential fate decisions. iTALK ligand-receptor axes within cell-type immune microenvironment Results Based on analysis, we four their associated subclusters, along exhibiting significant differential expression these subclusters. significantly enriched linked IS, while differentiation subpopulations different. Additionally, multiple receptor-ligand identified, indicating diverse interactions Conclusion demonstrated that phagocytosis critically influences disease progression. It also infarct cells. These findings provide valuable insights into underlying highlight pathways therapeutic intervention.

Язык: Английский

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