International Journal of General Medicine,
Год журнала:
2021,
Номер
Volume 14, С. 6305 - 6313
Опубликована: Окт. 1, 2021
Purpose:
Acute
ischemic
stroke
(AIS)
is
a
leading
health
problem
caused
by
cerebral
ischemia/reperfusion
(CI/R).
This
study
aimed
to
unveil
the
potential
clinical
value
and
mechanism
of
lncRNA
CASC15.
Patients
Methods:
The
expression
CASC15,
miR-338-3p
was
detected
quantitative
real-time
PCR
(qRT-PCR).
correlations
between
CASC15
national
institutes
scale
(NIHSS)
scores
or
were
evaluated
Pearson
correlation.
A
receiver
operating
characteristic
(ROC)
curve
performed
provide
diagnostic
Cell
Counting
Kit-8
(CCK-8)
flow
cytometer
used
detect
condition
cell
viability
apoptosis.
levels
interleukin-1beta
(IL-1β),
interleukin-6
(IL-6),
tumor
necrosis
factor-alpha
(TNF-α)
enzyme-linked
immunosorbent
assay
(ELISA)
assay.
Results:
increased
decreased
in
AIS
patients.
positive
association
NIHSS
score
an
inverse
revealed
might
discriminate
patients
from
healthy
people.
Silenced
exerted
neuroprotective
roles
on
viability,
apoptosis,
inflammation
via
miR-338-3p/ETS1
axis.
Conclusion:
act
as
biomarker
for
CASC15/miR-338-3p/ETS1
axis
played
essential
role
neuroinflammation.
Keywords:
CASC15/miR-338-3p/ETS1,
acute
stroke,
biomarker,
Metabolic Brain Disease,
Год журнала:
2025,
Номер
40(5)
Опубликована: Май 13, 2025
Long
noncoding
RNA
(lncRNA)
MEG3
has
been
considered
as
a
novel
target
for
alleviating
the
brain
tissue
damage
during
cerebral
ischemia-reperfusion
injury
(CIRI).
Numerous
studies
have
reported
that
pyroptosis
is
involved
in
pathogenesis
of
CIRI.
This
study
focused
on
whether
modulates
CIRI
via
and
its
underlying
mechanism.
The
middle
artery
occlusion/reperfusion
(MCAO/R)
mouse
model
oxygen
glucose
deprivation/reoxygenation
(OGD/R)
cell
were
established.
si-MEG3
miR-145-5p
inhibitor
transfected
to
inhibit
miR-145-5p,
respectively.
As
TLR4
inhibitor,
Resatorvid
inhibits
signaling
pathway.
TTC
TUNEL
staining
used
infarction
volume
death
detection.
differential
expression
MGE3,
TLR4,
NLRP3,
Caspase-1,
IL-1β,
IL-18
was
determined
using
real-time
PCR
western
blot.
interaction
between
well
confirmed
by
dual-luciferase
reporter
assay.
elevated
CIRI,
it
contributes
regulating
miR-145-5p/TLR4
axis.
knockdown
reduced
IL-18,
thereby
preventing
pyroptosis.
Inhibition
reversed
effect
promoted
Resatorvid,
counteracted
suppressed
Our
findings
reveal
promotes
miR-145-5p/TLR4/NLRP3
axis
aggravates
suggesting
potential
therapeutic
ischemic
stroke.
Aging,
Год журнала:
2021,
Номер
13(15), С. 19587 - 19597
Опубликована: Авг. 13, 2021
Differential
expression
and
diagnostic
significance
of
the
long
noncoding
RNA
(lncRNA)
AC136007.2
has
been
reported
in
patients
with
acute
ischemic
stroke
(AIS).
However,
its
role
on
disease
progression
outcome
remains
unclear.
Here,
we
employed
an
oxygen-glucose
deprivation/reperfusion
(OGD/R)
model
neuronal
SH-SY5Y
cells
performed
middle
cerebral
artery
occlusion
(MCAO)
surgery
rats
to
investigate
function
ischemia-reperfusion
(I/R)
injury.
was
determined
by
RT-qPCR
cell
viability
examined
using
CCK-8,
Edu,
LDH,
apoptosis
assays.
Pro-inflammatory
cytokine
assessed
ELISA.
OGD/R
downregulated
cells,
decreased
inducing
apoptosis,
stimulated
secretion
TNF-α,
IL-6,
IL-1β.
In
turn,
lentivirus-mediated
overexpression
significantly
reversed
these
phenomena.
LC3
immunofluorescence
western
blotting
analyses
LC3-I/II
Beclin-1
AMPK/mTOR
phosphorylation
status
showed
that
suppressed
autophagy
via
inactivation
signaling.
Notably,
incubation
AMPK
activator
AICAR
abolished
pro-survival
effect
upon
treatment.
Importantly,
intraventricular
injection
reduced
infarction
brain
edema
MCAO
rats,
as
shown
TTC
staining
water
content
measurements.
We
conclude
alleviates
I/R
injury
suppressing
AMPK/mTOR-dependent
autophagy.
Clinical Hemorheology and Microcirculation,
Год журнала:
2023,
Номер
84(3), С. 247 - 262
Опубликована: Фев. 28, 2023
BACKGROUND:
Circular
RNA
(circRNA)
has
been
found
to
play
an
important
role
in
the
progression
of
many
diseases,
including
ischemic
stroke.
However,
regulatory
mechanism
circSEC11A
stroke
need
further
investigation.
METHODS:
Human
brain
microvascular
endothelial
cells
(HBMECs)
were
stimulated
by
oxygen
glucose
deprivation
(OGD).
CircSEC11A,
SEC11A
mRNA
and
miR
(microRNA)-29a-3p
quantified
quantitative
real-time
PCR
(qRT-PCR).
SEMA3A,
BAX
BCL2
protein
level
was
western
blot.
Oxidative
stress,
cell
proliferation,
angiogenesis
apoptosis
abilities
gauged
oxidative
stress
assay
kit,
5-Ethynyl-2’-Deoxyuridine
(EdU)
staining,
tube
formation
flow
cytometry
assays,
respectively.
Direct
relationship
between
miR-29a-3p
or
SEMA3A
validated
dual-luciferase
reporter
assay,
RIP
pull-down
assay.
RESULTS:
CircSEC11A
upregulated
OGD-induced
HBMECs.
OGD
promoted
inhibited
proliferation
angiogenesis,
while
knockdown
relieved
effects.
functioned
as
sponge
for
miR-29a-3p,
inhibitor
reversed
effects
si-circSEC11A
on
HBMECs
injuries.
Moreover,
served
target
gene
miR-29a-3p.
MiR-29a-3p
inhibition
ameliorated
injuries,
overexpression
rescued
impacts
mimic.
CONCLUSION:
malignant
through
mediation
miR-29a-3p/SEMA3A
axis.
This
study
provided
new
insight
into
underlying
application
model
International Journal of General Medicine,
Год журнала:
2021,
Номер
Volume 14, С. 6305 - 6313
Опубликована: Окт. 1, 2021
Purpose:
Acute
ischemic
stroke
(AIS)
is
a
leading
health
problem
caused
by
cerebral
ischemia/reperfusion
(CI/R).
This
study
aimed
to
unveil
the
potential
clinical
value
and
mechanism
of
lncRNA
CASC15.
Patients
Methods:
The
expression
CASC15,
miR-338-3p
was
detected
quantitative
real-time
PCR
(qRT-PCR).
correlations
between
CASC15
national
institutes
scale
(NIHSS)
scores
or
were
evaluated
Pearson
correlation.
A
receiver
operating
characteristic
(ROC)
curve
performed
provide
diagnostic
Cell
Counting
Kit-8
(CCK-8)
flow
cytometer
used
detect
condition
cell
viability
apoptosis.
levels
interleukin-1beta
(IL-1β),
interleukin-6
(IL-6),
tumor
necrosis
factor-alpha
(TNF-α)
enzyme-linked
immunosorbent
assay
(ELISA)
assay.
Results:
increased
decreased
in
AIS
patients.
positive
association
NIHSS
score
an
inverse
revealed
might
discriminate
patients
from
healthy
people.
Silenced
exerted
neuroprotective
roles
on
viability,
apoptosis,
inflammation
via
miR-338-3p/ETS1
axis.
Conclusion:
act
as
biomarker
for
CASC15/miR-338-3p/ETS1
axis
played
essential
role
neuroinflammation.
Keywords:
CASC15/miR-338-3p/ETS1,
acute
stroke,
biomarker,
