Research progress on the mechanism of action and clinical application of remote ischemic post-conditioning for acute ischemic stroke

Clinical Neurology and Neurosurgery, Journal Year: 2024, Volume and Issue: 244, P. 108397 - 108397

Published: June 23, 2024

Language: Английский

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Knockdown of XIST Attenuates Cerebral Ischemia/Reperfusion Injury Through Regulation of miR-362/ROCK2 Axis

Neurochemical Research, Journal Year: 2021, Volume and Issue: unknown

Published: May 26, 2021

Language: Английский

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LncRNA AC136007.2 alleviates cerebral ischemic-reperfusion injury by suppressing autophagy

Aging, Journal Year: 2021, Volume and Issue: 13(15), P. 19587 - 19597

Published: Aug. 13, 2021

Differential expression and diagnostic significance of the long noncoding RNA (lncRNA) AC136007.2 has been reported in patients with acute ischemic stroke (AIS). However, its role on disease progression outcome remains unclear. Here, we employed an oxygen-glucose deprivation/reperfusion (OGD/R) model neuronal SH-SY5Y cells performed middle cerebral artery occlusion (MCAO) surgery rats to investigate function ischemia-reperfusion (I/R) injury. was determined by RT-qPCR cell viability examined using CCK-8, Edu, LDH, apoptosis assays. Pro-inflammatory cytokine assessed ELISA. OGD/R downregulated cells, decreased inducing apoptosis, stimulated secretion TNF-α, IL-6, IL-1β. In turn, lentivirus-mediated overexpression significantly reversed these phenomena. LC3 immunofluorescence western blotting analyses LC3-I/II Beclin-1 AMPK/mTOR phosphorylation status showed that suppressed autophagy via inactivation signaling. Notably, incubation AMPK activator AICAR abolished pro-survival effect upon treatment. Importantly, intraventricular injection reduced infarction brain edema MCAO rats, as shown TTC staining water content measurements. We conclude alleviates I/R injury suppressing AMPK/mTOR-dependent autophagy.

Language: Английский

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CircSEC11A knockdown alleviates oxidative stress and apoptosis and promotes cell proliferation and angiogenesis by regulating miR-29a-3p/SEMA3A axis in OGD-induced human brain microvascular endothelial cells (HBMECs)

Clinical Hemorheology and Microcirculation, Journal Year: 2023, Volume and Issue: 84(3), P. 247 - 262

Published: Feb. 28, 2023

BACKGROUND: Circular RNA (circRNA) has been found to play an important role in the progression of many diseases, including ischemic stroke. However, regulatory mechanism circSEC11A stroke need further investigation. METHODS: Human brain microvascular endothelial cells (HBMECs) were stimulated by oxygen glucose deprivation (OGD). CircSEC11A, SEC11A mRNA and miR (microRNA)-29a-3p quantified quantitative real-time PCR (qRT-PCR). SEMA3A, BAX BCL2 protein level was western blot. Oxidative stress, cell proliferation, angiogenesis apoptosis abilities gauged oxidative stress assay kit, 5-Ethynyl-2’-Deoxyuridine (EdU) staining, tube formation flow cytometry assays, respectively. Direct relationship between miR-29a-3p or SEMA3A validated dual-luciferase reporter assay, RIP pull-down assay. RESULTS: CircSEC11A upregulated OGD-induced HBMECs. OGD promoted inhibited proliferation angiogenesis, while knockdown relieved effects. functioned as sponge for miR-29a-3p, inhibitor reversed effects si-circSEC11A on HBMECs injuries. Moreover, served target gene miR-29a-3p. MiR-29a-3p inhibition ameliorated injuries, overexpression rescued impacts mimic. CONCLUSION: malignant through mediation miR-29a-3p/SEMA3A axis. This study provided new insight into underlying application model

Language: Английский

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Knockdown of RMST Impedes Neuronal Apoptosis and Oxidative Stress in OGD/R-Induced Ischemic Stroke Via Depending on the miR-377/SEMA3A Signal Network

Neurochemical Research, Journal Year: 2021, Volume and Issue: 46(3), P. 584 - 594

Published: Jan. 6, 2021

Language: Английский

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