Acta veterinaria,
Год журнала:
2023,
Номер
73(4), С. 486 - 501
Опубликована: Дек. 1, 2023
Abstract
Alterations
in
the
serum
protein
pattern
may
be
associated
with
many
diseases,
including
neoplastic
processes.
In
veterinary
medicine,
these
changes
are
poorly
understood.
Therefore,
this
study
was
aimed
at
analysis
of
distribution
blood
fractions
separated
by
agarose
gel
electrophoresis,
and
determination
concentrations
main
acute
phase
proteins
bitches
mammary
gland
neoplasia.
The
evaluation
conducted
on
twelve
female
dogs
palpable
single
or
multiple
nodules
parenchyma
ten
tumor-free
clinically
healthy
to
compare
possible
differences
obtained
results.
Blood
used
perform
electrophoresis
analyze
total
following
canine
proteins:
amyloid
A,
haptoglobin,
C-reactive
α
1
-acid
glycoprotein.
were
slightly
higher
tumors.
Serum
showed
lower
mean
albumin
-globulins
affected
dogs,
while
2
-and
β
significantly
(P=0.0032
P=0.0021,
respectively)
compared
without
proteins,
haptoglobin
tumors
(P=0.0025
P=0.0002,
respectively).
values
glycoprotein
did
not
vary
markedly
between
Presented
data
suggest
that
processes
also
alter
electrophoretic
induce
production
some
inflammatory
proteins.
Frontiers in Oncology,
Год журнала:
2022,
Номер
12
Опубликована: Сен. 27, 2022
Background
Glioma
is
the
most
common
primary
malignant
brain
tumor
with
high
mortality
and
poor
prognosis.
Hepcidin
a
fascinating
iron
metabolism
regulator.
However,
prognostic
value
of
hepcidin
HAMP
in
gliomas
its
correlation
immune
cell
infiltration
remain
unclear.
Here,
we
comprehensively
elucidate
potential
role
gliomas.
Methods
gene
expression
clinical
characteristics
glioma
were
analyzed
using
CGGA,
TCGA,
Rembrandt
Gravendeel
databases.
A
survival
analysis
was
conducted
Kaplan–Meier
Cox
regression
analyses.
set
enrichment
(GSEA)
to
select
pathways
significantly
enriched
for
associations.
The
correlations
between
immunotherapy
network
platforms
such
as
CIBERSORT
TIMER.
Results
In
tissues,
increased.
High
related
grade,
age,
PRS
type,
IDH
mutation,
chemotherapy
status
1p19q
codeletion
status,
which
indicates
prognosis
patients.
can
be
used
an
independent
factor
through
multivariate
COX
analysis.
results
Gene
Ontology
(GO),
Kyoto
Encyclopedia
Genome
(KEGG)
indicated
that
involved
response.
addition,
positively
correlated
degree
infiltration,
various
markers
efficacy
immunotherapy.
Conclusion
Our
indicate
candidate
biomarker
judge
invasion
Frontiers in Pharmacology,
Год журнала:
2023,
Номер
14
Опубликована: Май 22, 2023
Background:
Stomach
adenocarcinoma
(STAD)
is
the
fourth
highest
cause
of
cancer
mortality
worldwide.
Alterations
in
copper
metabolism
are
closely
linked
to
genesis
and
progression.
We
aim
identify
prognostic
value
metabolism-related
genes
(CMRGs)
STAD
characteristic
tumor
immune
microenvironment
(TIME)
CMRG
risk
model.
Methods:
CMRGs
were
investigated
cohort
from
The
Cancer
Genome
Atlas
(TCGA)
database.
Then,
hub
screened
out
with
LASSO
Cox
regression,
followed
by
establishment
a
model
validated
GSE84437
Expression
Omnibus
(GEO)
then
utilized
create
nomogram.
TMB
(tumor
mutation
burden)
cell
infiltration
investigated.
To
validate
immunotherapy
response
prediction,
immunophenoscore
(IPS)
IMvigor210
employed.
Finally,
data
single-cell
RNA
sequencing
(scRNA-seq)
was
depict
properties
CMRGs.
Results:
There
75
differentially
expressed
identified,
6
which
OS.
5
selected
construction
High-risk
patients
had
shorter
life
expectancy
than
those
low-risk.
score
independently
predicted
survival
through
univariate
multivariate
regression
analyses,
ROC
calculation
generating
results.
This
immunocyte
showed
good
prediction
performance
for
patients'
survival.
Furthermore,
high-risk
group
lower
somatic
counters
higher
TIDE
scores,
but
low-risk
greater
IPS-PD-1
IPS-CTLA4
indicating
checkpoint
inhibitors
(ICIs)
response,
corroborated
cohort.
low
high
differential
susceptibility
anticancer
drugs.
Based
on
CMRGs,
two
subclusters
identified.
Cluster
2
superior
clinical
TIME
concentrated
endothelium,
fibroblasts,
macrophages.
Conclusion:
promising
biomarker
prognosis
can
be
used
as
guide
immunotherapy.
Frontiers in Pharmacology,
Год журнала:
2023,
Номер
14
Опубликована: Авг. 11, 2023
Glioma
is
regarded
as
a
prevalent
form
of
cancer
that
affects
the
Central
Nervous
System
(CNS),
with
an
aggressive
growth
pattern
and
low
clinical
cure
rate.
Despite
advancement
treatment
strategy
surgical
resection,
chemoradiotherapy
immunotherapy
in
last
decade,
outcome
still
grim,
which
ascribed
to
immunogenicity
tumor
microenvironment
(TME)
glioma.
The
multifunctional
molecule,
called
ceruloplasmin
(CP)
involved
iron
metabolism.
Its
expression
pattern,
prognostic
significance,
association
immune
cells
gliomas
have
not
been
thoroughly
investigated.
Studies
using
variety
databases,
including
Chinese
Genome
Atlas
(CGGA),
Cancer
(TCGA),
Gliovis,
showed
mRNA
protein
levels
CP
patients
suffering
from
glioma
increased
significantly
increasing
grade.
Kaplan-Meier
(KM)
curves
statistical
tests
highlighted
significant
reduction
survival
time
elevated
levels.
According
Cox
regression
analysis,
can
be
utilized
stand-alone
predictive
biomarker
A
between
numerous
immune-related
pathways
was
found
after
analyzing
data
Gene
Ontology
(GO),
Kyoto
Encyclopedia
Genes
Genomes
(KEGG),
Set
Enrichment
Analysis
(GSEA).
Tumor
Immune
Estimation
Resource
(TIMER)
CIBERSORT
analyses
indicated
substantial
correlation
infiltration
immunocytes
TME.
Additionally,
checkpoints
favorable
correlation.
these
results,
better
prognoses
cell
when
their
low.
As
result,
could
used
probable
therapeutic
target
for
potentially
anticipate
effectiveness
immunotherapy.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 4, 2024
Abstract
Triple-negative
breast
cancer
(TNBC)
lacks
estrogen,
progesterone,
and
human
epidermal
growth
factor
receptors
has
a
poor
prognosis
as
it
is
resistant
to
chemotherapy.
A
new
treatment
option
for
this
type
of
may
be
by
putting
these
malignant
cells
into
dormancy.
The
oocyte’s
embryonic
milieu
presents
unique
tumor
reversion
microenvironment
inducing
arrest
changing
cells’
phenotypes.
We
conducted
an
in-silico
study
determine
the
most
likely
oocyte
extract
(OE)
proteins
involved
in
dormancy
using
HDock,
CluPro,
molecular
dynamic
(MD)
simulation.
Results
showed
low
energy
scores
complexes
between
OE
four
surface
markers:
K1C14,
CLD3,
CLD4,
ITA6.
Apolipoprotein
A1
(APOA1)
C3
(APOC3)
highest
stability
affinity
with
These
are
key
tumor-related
pathways
such
angiogenesis,
proliferation,
apoptosis,
migration.
This
will
pave
way
exploring
novel
therapeutic
options
induce
TNBC
cells.
Acta veterinaria,
Год журнала:
2023,
Номер
73(4), С. 486 - 501
Опубликована: Дек. 1, 2023
Abstract
Alterations
in
the
serum
protein
pattern
may
be
associated
with
many
diseases,
including
neoplastic
processes.
In
veterinary
medicine,
these
changes
are
poorly
understood.
Therefore,
this
study
was
aimed
at
analysis
of
distribution
blood
fractions
separated
by
agarose
gel
electrophoresis,
and
determination
concentrations
main
acute
phase
proteins
bitches
mammary
gland
neoplasia.
The
evaluation
conducted
on
twelve
female
dogs
palpable
single
or
multiple
nodules
parenchyma
ten
tumor-free
clinically
healthy
to
compare
possible
differences
obtained
results.
Blood
used
perform
electrophoresis
analyze
total
following
canine
proteins:
amyloid
A,
haptoglobin,
C-reactive
α
1
-acid
glycoprotein.
were
slightly
higher
tumors.
Serum
showed
lower
mean
albumin
-globulins
affected
dogs,
while
2
-and
β
significantly
(P=0.0032
P=0.0021,
respectively)
compared
without
proteins,
haptoglobin
tumors
(P=0.0025
P=0.0002,
respectively).
values
glycoprotein
did
not
vary
markedly
between
Presented
data
suggest
that
processes
also
alter
electrophoretic
induce
production
some
inflammatory
proteins.
