Dioscin promotes autophagy by regulating the AMPK-mTOR pathway in ulcerative colitis

Immunopharmacology and Immunotoxicology, Год журнала: 2022, Номер 44(2), С. 238 - 246

Опубликована: Фев. 17, 2022

Dioscin is reported to alleviate the dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) in mice. Autophagy plays an anti-inflammatory role UC. We herein aimed explore biological functions of dioscin autophagy UC.To effects on UC progression, a DSS-induced mouse model was established. Body weight, disease activity index and macroscopic damage scores were recorded for seven days. Hematoxylin & Eosin (HE) staining used stain colon sections BX53 microscope prepared observe pathological changes. The activities glutathione, superoxidative dismutase, malondialdehyde determined by commercially available kits. Western blotting performed measure protein levels p-AMPK/AMPK, p-mTOR/mTOR autophagy-related genes.The oxidative stress mice ameliorated after treatment. promoted phosphorylation AMPK inhibit mTOR activation facilitated UC.Dioscin promotes promoting colitis.

Язык: Английский

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Trifluoperazine activates AMPK / mTOR / ULK1 signaling pathway to induce mitophagy in osteosarcoma cells

Chemico-Biological Interactions, Год журнала: 2024, Номер 392, С. 110904 - 110904

Опубликована: Фев. 13, 2024

Язык: Английский

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Homogeneous Polyporus polysaccharide exerts anti-bladder cancer effects via autophagy induction

Pharmaceutical Biology, Год журнала: 2024, Номер 62(1), С. 214 - 221

Опубликована: Фев. 14, 2024

Context Polyporus polysaccharide (PPS), the leading bioactive ingredient extracted from umbellatus (Pers.) Fr. (Polyporaceae), has been demonstrated to exert anti-bladder cancer and immunomodulatory functions in macrophages.

Язык: Английский

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Multifunctional Roles of Betulinic Acid in Cancer Chemoprevention: Spotlight on JAK/STAT, VEGF, EGF/EGFR, TRAIL/TRAIL-R, AKT/mTOR and Non-Coding RNAs in the Inhibition of Carcinogenesis and Metastasis

Molecules, Год журнала: 2022, Номер 28(1), С. 67 - 67

Опубликована: Дек. 21, 2022

The pursual of novel anticancer molecules from natural sources has gained worthwhile appreciation, and a significant fraction conceptual knowledge revolutionized our understanding about heterogeneous nature cancer. Betulinic acid fascinated interdisciplinary researchers due to its tremendous pharmacological properties. Ground-breaking discoveries have unraveled previously unprecedented empirical proof-of-concept momentous chemopreventive role betulinic against carcinogenesis metastasis. Deregulation cell signaling pathways been reported play linchpin in cancer progression colonization metastatically competent cells the distant organs for development secondary tumors. Importantly, demonstrated unique properties mechanistically modulate oncogenic transduction cascades. In this mini-review, we attempted provide sophisticated compendium regulatory chemoprevention. We partitioned multi-component review into different sections which summarized landmark research-works highlighted mediated regulation JAK/STAT, VEGF, EGF/EGFR, TRAIL/TRAIL-R, AKT/mTOR ubiquitination inhibition parallel, cascades non-coding RNAs will be critically analyzed culture animal model studies. Better comprehension pharmaceutical features mapping existing gaps valuable translatability preclinical studies rationally designed clinical trials.

Язык: Английский

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Isoprenoid flavonoids isolated from Sophora davidii and their activities induces apoptosis and autophagy in HT29 cells

Fitoterapia, Год журнала: 2024, Номер 175, С. 105945 - 105945

Опубликована: Апрель 2, 2024

Язык: Английский

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Ziziphus mauritiana Leaf Extract Induces ROS-dependent Apoptosis by Downregulation of CD81 in MDA- MB 231 Triple Negative Breast Cancer Cells

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Background. Breast cancer (BC) is the most prevalent among women, with triple-negative breast (TNBC) representing a particularly aggressive subtype. Current treatments often have limited efficacy and significant toxicity, highlighting need for alternative therapeutic strategies. Ziziphus mauritiana (ZM) leaf extract contains triterpenoids which been proven to be used as anticancer agents, but not studied in TNBC cells. Aim. To examine triterpenoid content ZM, especially BA OA, ZM cytotoxicity, intracellular ROS levels, amount of apoptosis, CD81 expression on MDA-MB 231 cells after being given ZM. Material Methods. The was prepared using sequential extraction method. presence confirmed via HPLC LC-MS analysis. cytotoxicity assessed CCK-8 assay, while flow cytometry employed analyze levels. Apoptosis quantified an Annexin V-FITC/PI detection kit. Results. Based HPLC, 28.3 µg/ml, OA 13.4 µg/ml. significantly reduced cell viability by 28.28% at 400 µg/ml 48 hours. Flow revealed notable downregulation (p < 0.0001) increase levels 0.0001). Furthermore, induced early late apoptosis treated cells, increases 2.34-fold 2.66-fold, respectively. Conclusion. exhibits potent cytotoxic effects against through ROS-dependent downregulation, suggesting its potential novel agent treatment.

Язык: Английский

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Anticancer Properties of Dillenia suffruticosa (Griffith Ex. Hook. F. & Thomsom) Martelli

Phytomedicine Plus, Год журнала: 2025, Номер unknown, С. 100736 - 100736

Опубликована: Янв. 1, 2025

Язык: Английский

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Enhanced ZBTB10 expression induced by betulinic acid inhibits gastric cancer progression by inactivating the ARRDC3/ITGB4/PI3K/AKT pathway

Cellular Oncology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Язык: Английский

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Review: performance of jujube and its extracts in cancer: therapeutic, toxicity-reducing and potentiating effects

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Янв. 29, 2025

Cancer is one of the most serious public health challenges in world. The number new and fatal patients with cancer continues to increase every year, which poses a threat human health. Although there are effective treatments such as radiotherapy chemotherapy for cancers, they often accompanied by side effects. With development pharmaceutical industry, gradual desire develop low-toxicity anticancer drugs from traditional Chinese herbs. Due its “homology medicine food,” jujube herbs that extensively used China. In addition, has received much attention around world excellent pharmacological effects food value. This article reviews fruit, food, drug, jujube, have been shown current studies, analyzes therapeutic mechanism, active ingredients, extraction isolation methods, well synergistic attenuation performance cancer. will further promote progress natural medicine’s anti-cancer effect.

Язык: Английский

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COTI-2 suppresses the malignancy of bladder cancer by inducing apoptosis via the AMPK-mTOR signaling pathway.

PubMed, Год журнала: 2025, Номер 28(3), С. 240 - 246

Опубликована: Янв. 1, 2025

COTI-2, an innovative oral homocysteine, has shown promising antitumor results on multiple types of cancer. However, its effects in treating bladder cancer (BCa) and the underlying molecular mechanisms have not been elucidated. The present study aimed to explore COTI-2 BCa potential mechanisms. cell lines, including 5637 T24 were treated with at concentrations 0.5 1 μM, respectively. Cell Counting Kit (CCK)-8 assay, colony formation apoptosis transwell migration invasion assay conducted evaluate cells. Western blotting, H&E, immunohistochemical staining, immunofluorescence analysis performed investigate Moreover, a xenograft model nude mice using cells was generated determine activities vivo. highly inhibited proliferation cells, induced their apoptosis. it efficiently suppressed Additionally, subcutaneous showed that treatment tumor growth by inducing We also found promoted presumably through activating AMPK/mTOR pathway. Our data suggest effectively reduces malignancy BCa, probably via signaling These highlight as therapeutic agent for BCa.

Язык: Английский

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