Cancers,
Год журнала:
2020,
Номер
13(1), С. 84 - 84
Опубликована: Дек. 30, 2020
Exosomes
are
lipid
bilayer
particles
released
from
cells
into
their
surrounding
environment.
These
vesicles
mediators
of
near
and
long-distance
intercellular
communication
affect
various
aspects
cell
biology.
In
addition
to
biological
function,
they
play
an
increasingly
important
role
both
in
diagnosis
as
therapeutic
agents.
this
paper,
we
review
recent
literature
related
the
molecular
composition
exosomes,
paying
special
attention
pathogenesis,
along
with
application
biomarkers
tools.
context,
analyze
potential
use
exosomes
biomedicine,
well
limitations
that
preclude
wider
application.
Journal of Hematology & Oncology,
Год журнала:
2015,
Номер
8(1)
Опубликована: Июль 9, 2015
Exosomes
have
emerged
as
a
novel
mode
of
intercellular
communication.
can
shuttle
bioactive
molecules
including
proteins,
DNA,
mRNA,
well
non-coding
RNAs
from
one
cell
to
another,
leading
the
exchange
genetic
information
and
reprogramming
recipient
cells.
Increasing
evidence
suggests
that
tumor
cells
release
excessive
amount
exosomes,
which
may
influence
initiation,
growth,
progression,
metastasis,
drug
resistance.
In
addition,
exosomes
transfer
message
immune
stromal
cells,
contributing
escape
surveillance
formation
niche.
this
review,
we
highlight
recent
advances
in
biology
cancer
communicasomes.
We
review
multifaceted
roles
small
secreted
particles,
communicating
with
other
within
microenvironment.
Given
are
type
specific,
stable,
accessible
body
fluids,
provide
promising
biomarkers
for
diagnosis
represent
new
targets
therapy.
Journal of Extracellular Vesicles,
Год журнала:
2016,
Номер
5(1)
Опубликована: Янв. 1, 2016
Intercommunication
between
cancer
cells
and
with
their
surrounding
distant
environments
is
key
to
the
survival,
progression
metastasis
of
tumour.
Exosomes
play
a
role
in
this
communication
process.
MicroRNA
(miRNA)
expression
frequently
dysregulated
tumour
can
be
reflected
by
distinct
exosomal
miRNA
(ex‐miRNA)
profiles
isolated
from
bodily
fluids
patients.
Here,
potential
ex‐miRNA
as
biomarker
therapeutic
target
critically
analysed.
are
stable
source
but,
despite
number
methods
for
exosome
extraction
quantification,
suitability
diagnostics
clinical
setting
questionable.
Furthermore,
exosomally
transferred
miRNAs
alter
behaviour
recipient
stromal
promote
oncogenesis,
highlighting
cell
cancer.
However,
our
incomplete
understanding
biogenesis
loading
mechanisms
means
that
strategies
exosomes
or
limited
not
specific
cells.
Therefore,
if
employed
novel
non‐invasive
diagnostic
approaches
cancer,
two
further
advances
necessary:
isolate
detect
ex‐miRNA,
better
functions
tumour‐cell
communication.
EBioMedicine,
Год журнала:
2018,
Номер
36, С. 209 - 220
Опубликована: Сен. 20, 2018
Cancer-associated
fibroblasts
(CAFs)
play
an
important
role
in
regulating
tumor
progression
by
transferring
exosomes
to
neighboring
cells.
Our
aim
was
clarify
the
of
microRNA
encapsulated
derived
from
CAFs
oral
squamous
cell
carcinoma
(OSCC).We
examined
expression
profiles
and
donor-matched
normal
(NFs)
patients
with
OSCC.
We
used
confocal
microscopy
examine
transportation
exosomal
miR-34a-5p
between
OSCC
Next,
luciferase
reporter
its
mutant
plasmids
were
confirm
direct
target
gene
miR-34a-5p.
Phenotypic
assays
vivo
growth
experiments
investigate
functional
significance
miR-34a-5p.We
found
that
CAF-derived
significantly
reduced,
could
transfer
In
xenograft
experiments,
overexpression
inhibit
tumorigenesis
further
revealed
binds
downstream
AXL
suppress
proliferation
metastasis.
Stable
ectopic
cells
overexpressing
restored
motility
abolished
miRNA.
The
miR-34a-5p/AXL
axis
promoted
via
AKT/GSK-3β/β-catenin
signaling
pathway,
which
induce
epithelial-mesenchymal
transition
(EMT)
promote
cancer
enhanced
nuclear
translocation
β-catenin
then
induced
transcriptional
upregulation
SNAIL,
turn
activated
both
MMP-2
MMP-9.The
confers
aggressiveness
through
AKT/GSK-3β/β-catenin/Snail
cascade
might
represent
a
therapeutic
for
FUND:
National
Natural
Science
Foundation
China.
International Journal of Molecular Sciences,
Год журнала:
2017,
Номер
18(6), С. 1122 - 1122
Опубликована: Май 24, 2017
There
remains
an
urgent
need
for
novel
therapeutic
strategies
to
treat
metastatic
cancer,
which
results
in
over
8
million
deaths
annually
worldwide.
Following
secretion,
exosomes
are
naturally
taken
up
by
cells,
and
capable
of
the
stable
transfer
drugs,
microRNAs
proteins.
As
knowledge
biogenesis,
release
uptake
continues
evolve,
thus
also
has
interest
these
extracellular
vesicles
as
potential
tumor-targeted
vehicles
cancer
therapy.
The
ability
engineer
exosome
content
migratory
itinerary
holds
tremendous
promise.
Studies
date
have
employed
viral
non-viral
methods
parent
cells
secrete
modified
exosomes,
or
alternatively,
directly
manipulate
following
secretion.
majority
studies
demonstrated
promising
results,
with
decreased
tumor
cell
invasion,
migration
proliferation,
along
enhanced
immune
response,
death,
sensitivity
chemotherapy
observed.
outlined
this
review
highlight
exciting
treatment.
Successful
implementation
clinical
setting
will
be
dependent
upon
establishment
rigorous
standards
manipulation,
isolation,
characterisation.
Bioengineering & Translational Medicine,
Год журнала:
2017,
Номер
2(2), С. 170 - 179
Опубликована: Июнь 1, 2017
Mesenchymal
stem
cell
(MSC)-derived
extracellular
vesicles
(EVs)
have
emerged
as
potential
therapeutic
agents
for
numerous
applications.
EVs
offer
advantages
over
cell-based
therapies
with
regard
to
safety,
stability
and
clearance
profiles,
however
production
potency
limitations
must
be
addressed
enable
eventual
translation
of
EV-based
approaches.
Thus,
we
sought
examine
the
role
specific
culture
parameters
on
MSC
EV
bioactivity
toward
informing
rational
design
scalable
biomanufacturing.
We
report
significantly
reduced
vascularization
bioactivity,
measured
by
an
endothelial
gap
closure
assay,
increasing
passage
in
trypsinization,
especially
beyond
4.
further
show
that
increased
frequency
collection
yielded
higher
numbers
from
same
initial
number
MSCs
a
24
hr
period.
Finally,
demonstrate
decreased
seeding
density
flasks
resulted
per
other
types.
Overall,
these
studies
highlight
need
careful
consideration
at
laboratory
scale
large-scale
biomanufacturing
schemes.
Cell Death and Disease,
Год журнала:
2020,
Номер
11(11)
Опубликована: Ноя. 17, 2020
Abstract
Drug
resistance
is
a
daunting
challenge
in
the
treatment
of
breast
cancer
(BC).
Exosomes,
as
intercellular
communicative
vectors
tumor
microenvironment,
play
an
important
role
BC
progression.
With
in-depth
understanding
heterogeneity,
emerging
exosomes
drug
has
attracted
extensive
attention.
The
functional
proteins
or
non-coding
RNAs
contained
secreted
from
and
stromal
cells
mediate
by
regulating
efflux
metabolism,
pro-survival
signaling,
epithelial–mesenchymal
transition,
stem-like
property,
microenvironmental
remodeling.
In
this
review,
we
summarize
underlying
associations
between
discuss
unique
biogenesis
exosomes,
change
exosome
cargo,
pattern
release
response
to
treatment.
Moreover,
propose
candidate
biomarker
predicting
monitoring
therapeutic
potential
target
carrier
reverse
BC.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(17), С. 9952 - 9952
Опубликована: Сен. 1, 2022
Breast
cancer
(BC)
is
a
highly
heterogeneous
disease.
The
treatment
of
BC
complicated
owing
to
intratumoral
complexity.
Tissue
biopsy
and
immunohistochemistry
are
the
current
gold
standard
techniques
guide
breast
therapy;
however,
these
do
not
assess
tumoral
molecular
heterogeneity.
Personalized
medicine
aims
overcome
biological
clinical
complexities.
Advances
in
computational
analyses
have
enabled
increasingly
sensitive,
specific,
accurate
application
liquid
biopsy.
Such
progress
has
ushered
new
era
precision
medicine,
where
objective
personalized
cancer,
early
screening,
diagnosis
prognosis,
relapse
detection,
longitudinal
monitoring,
drug
selection.
Liquid
can
be
defined
as
sampling
components
tumor
cells
that
released
from
and/or
metastatic
deposits
into
blood,
urine,
feces,
saliva,
other
substances.
include
circulating
(CTCs),
DNA
(ctDNA)
or
RNA
(ctRNA),
platelets,
exosomes.
This
review
highlight
role
medicine.
Tumor Biology,
Год журнала:
2016,
Номер
37(9), С. 12169 - 12180
Опубликована: Май 25, 2016
Exosomes
are
nano-sized
membrane
vesicles
secreted
by
both
normal
and
cancer
cells.
Emerging
evidence
indicates
that
cells
derived
exosomes
contribute
to
progression
through
the
modulation
of
tumor
microenvironment.
However,
effects
from
gastric
on
macrophages
not
well
understood.
In
this
study,
we
investigated
biological
role
in
activation
macrophages.
We
demonstrated
activated
express
increased
levels
proinflammatory
factors,
which
turn
promoted
cell
proliferation
migration.
addition,
remarkably
upregulated
phosphorylation
NF-κB
Inhibiting
reversed
upregulation
factors
blocked
their
promoting
Moreover,
found
could
also
activate
human
peripheral
blood
monocytes
NF-κB.
conclusion,
our
results
suggest
stimulate
pathway
promote
progression,
provides
a
potential
therapeutic
approach
for
interfering
with
interaction
between