CD7‐targeting pro‐apoptotic extracellular vesicles: A novel approach for T‐cell haematological malignancy therapy DOI Creative Commons

Bei Zhang,

Jianqiang Chen,

Jiming Chen

и другие.

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(12)

Опубликована: Дек. 1, 2024

Abstract T‐cell haematological malignancies progress rapidly and have a high mortality rate effective treatments are still lacking. Here, we developed drug delivery system utilizing 293T cell‐derived extracellular vesicles (EVs) modified with an anti‐CD7 single‐chain variable fragment (αCD7/EVs). Given the challenges of chemotherapy resistance in patients malignancy, selected cytochrome C (CytC) Bcl2 siRNA ( siBcl2 ) as therapeutic agents loaded them into αCD7/EVs (αCD7/EVs/CytC/ ). We found that efficiently targeted were internalized by human T‐ALL Molt‐4 cells. In addition, interaction between αCD7 CD7 switched EV entry pathway cells from macropinocytosis‐dependent endocytosis to clathrin‐mediated endocytosis, thereby reducing EV‐lysosome colocalization, ultimately improving CytC efficiency increasing cytotoxicity nascent EVs EV‐treated Notably, αCD7/EVs/CytC/ demonstrated similar efficacy against both chemotherapy‐resistant (CR‐Molt‐4). Furthermore, exhibited safety, low immunogenicity minimal impact on T Therefore, promising approaches for treating + malignancies.

Язык: Английский

Inflammation-triggered Gli1+ stem cells engage with extracellular vesicles to prime aberrant neutrophils to exacerbate periodontal immunopathology DOI

Xinyue Cai,

Chen‐Xi Zheng,

Hao Guo

и другие.

Cellular and Molecular Immunology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Язык: Английский

Процитировано

1
Methods for Extracellular Vesicle Isolation: Relevance for Encapsulated miRNAs in Disease Diagnosis and Treatment DOI Open Access
María Ljungström, Elisa Oltra

Genes, Год журнала: 2025, Номер 16(3), С. 330 - 330

Опубликована: Март 12, 2025

Extracellular vesicles (EVs) are nanovesicles that facilitate intercellular communication by carrying essential biomolecules under physiological and pathological conditions including microRNAs (miRNAs). They found in various body fluids, such as blood, urine, saliva, their levels fluctuate with disease progression, making them valuable diagnostic tools. However, isolating EVs is challenging due to small size biological complexity. Here, we summarize the principles behind most common EV isolation methods ultracentrifugation, precipitation, immunoaffinity, sorting, ultrafiltration, exclusion chromatography, microfluidics while highlighting protocol strengths weaknesses. We also review main strategies identify quantify circulating miRNAs a particular focus on EV-encapsulated miRNAs. Since these hold special clinical interest derived from superior stability therapeutic potential, information provided here should provide guidance for future research initiatives promising field of treatment based

Язык: Английский

Процитировано

0
Extracellular Vesicles in Atherosclerosis: From Pathogenesis to Theranostic Applications DOI
Fei Fang,

Hanqiao Yang,

Xiaoli Wang

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

Abstract Atherosclerosis is a chronic vascular inflammatory disorder, and the cardiovascular disease resulting from it leading cause of death worldwide. Extracellular vesicles (EVs) are small membrane secreted by cells, playing crucial role in regulating intercellular communication, signal transduction, various pathophysiological processes. EVs have been shown to play significant progression regression atherosclerotic disease. Therefore, comprehensive understanding pathogenesis atherosclerosis essential for advancing knowledge this field driving development innovation novel diagnostic therapeutic strategies. This review summarizes pathological roles atherosclerosis, including their involvement endothelial cell inflammation, phenotypic transformation smooth muscle macrophage activation, foam formation, plaque thrombosis rupture. Furthermore, discusses innovative EV‐based strategies diagnosing treating atherosclerosis. Finally, challenges prospects translational research on discussed. work aims provide an EV‐centered perspective diagnosis treatment

Язык: Английский

Процитировано

0
Emerging role and translational potential of small extracellular vesicles in neuroscience DOI

Iswarya Shanmugam,

Sivani Radhakrishnan,

S. Winkins Santosh

и другие.

Life Sciences, Год журнала: 2024, Номер 355, С. 122987 - 122987

Опубликована: Авг. 14, 2024

Язык: Английский

Процитировано

1
CD7‐targeting pro‐apoptotic extracellular vesicles: A novel approach for T‐cell haematological malignancy therapy DOI Creative Commons

Bei Zhang,

Jianqiang Chen,

Jiming Chen

и другие.

Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(12)

Опубликована: Дек. 1, 2024

Abstract T‐cell haematological malignancies progress rapidly and have a high mortality rate effective treatments are still lacking. Here, we developed drug delivery system utilizing 293T cell‐derived extracellular vesicles (EVs) modified with an anti‐CD7 single‐chain variable fragment (αCD7/EVs). Given the challenges of chemotherapy resistance in patients malignancy, selected cytochrome C (CytC) Bcl2 siRNA ( siBcl2 ) as therapeutic agents loaded them into αCD7/EVs (αCD7/EVs/CytC/ ). We found that efficiently targeted were internalized by human T‐ALL Molt‐4 cells. In addition, interaction between αCD7 CD7 switched EV entry pathway cells from macropinocytosis‐dependent endocytosis to clathrin‐mediated endocytosis, thereby reducing EV‐lysosome colocalization, ultimately improving CytC efficiency increasing cytotoxicity nascent EVs EV‐treated Notably, αCD7/EVs/CytC/ demonstrated similar efficacy against both chemotherapy‐resistant (CR‐Molt‐4). Furthermore, exhibited safety, low immunogenicity minimal impact on T Therefore, promising approaches for treating + malignancies.

Язык: Английский

Процитировано

1