Cited by Emerging role and translational potential of small extracellular vesicles in neuroscience

Inflammation-triggered Gli1+ stem cells engage with extracellular vesicles to prime aberrant neutrophils to exacerbate periodontal immunopathology DOI

Xinyue Cai,

Chen‐Xi Zheng,

Hao Guo

et al.

Cellular and Molecular Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Language: Английский

Citations

Methods for Extracellular Vesicle Isolation: Relevance for Encapsulated miRNAs in Disease Diagnosis and Treatment DOI

María Ljungström,

Elisa Oltra

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 330 - 330

Published: March 12, 2025

Extracellular vesicles (EVs) are nanovesicles that facilitate intercellular communication by carrying essential biomolecules under physiological and pathological conditions including microRNAs (miRNAs). They found in various body fluids, such as blood, urine, saliva, their levels fluctuate with disease progression, making them valuable diagnostic tools. However, isolating EVs is challenging due to small size biological complexity. Here, we summarize the principles behind most common EV isolation methods ultracentrifugation, precipitation, immunoaffinity, sorting, ultrafiltration, exclusion chromatography, microfluidics while highlighting protocol strengths weaknesses. We also review main strategies identify quantify circulating miRNAs a particular focus on EV-encapsulated miRNAs. Since these hold special clinical interest derived from superior stability therapeutic potential, information provided here should provide guidance for future research initiatives promising field of treatment based

Language: Английский

Citations

CD7‐targeting pro‐apoptotic extracellular vesicles: A novel approach for T‐cell haematological malignancy therapy DOI

Bei Zhang,

Jianqiang Chen,

Jiming Chen

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(12)

Published: Dec. 1, 2024

Abstract T‐cell haematological malignancies progress rapidly and have a high mortality rate effective treatments are still lacking. Here, we developed drug delivery system utilizing 293T cell‐derived extracellular vesicles (EVs) modified with an anti‐CD7 single‐chain variable fragment (αCD7/EVs). Given the challenges of chemotherapy resistance in patients malignancy, selected cytochrome C (CytC) Bcl2 siRNA ( siBcl2 ) as therapeutic agents loaded them into αCD7/EVs (αCD7/EVs/CytC/ ). We found that efficiently targeted were internalized by human T‐ALL Molt‐4 cells. In addition, interaction between αCD7 CD7 switched EV entry pathway cells from macropinocytosis‐dependent endocytosis to clathrin‐mediated endocytosis, thereby reducing EV‐lysosome colocalization, ultimately improving CytC efficiency increasing cytotoxicity nascent EVs EV‐treated Notably, αCD7/EVs/CytC/ demonstrated similar efficacy against both chemotherapy‐resistant (CR‐Molt‐4). Furthermore, exhibited safety, low immunogenicity minimal impact on T Therefore, promising approaches for treating + malignancies.

Language: Английский

Citations

Emerging role and translational potential of small extracellular vesicles in neuroscience DOI

Iswarya Shanmugam,

Sivani Radhakrishnan,

S. Winkins Santosh

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 355, P. 122987 - 122987

Published: Aug. 14, 2024

Language: Английский

Citations