Artificial Insemination as an Alternative Transmission Route for African Swine Fever Virus
Pathogens,
Год журнала:
2022,
Номер
11(12), С. 1539 - 1539
Опубликована: Дек. 14, 2022
The
rapid
spread
of
the
African
swine
fever
virus
(ASFV),
causing
severe
disease
with
often
high
fatality
rates
in
Eurasian
suids,
prevails
as
a
threat
for
pig
populations
and
dependent
industries
worldwide.
Although
advancing
scientific
progress
continually
enhances
our
understanding
ASFV
pathogenesis,
alternative
transmission
routes
have
yet
to
be
assessed.
Here,
we
demonstrate
that
can
efficiently
transferred
from
infected
boars
naïve
recipient
gilts
through
artificial
insemination
(AI).
In
modern
production,
semen
boar
studs
supplies
many
sow
herds.
Thus,
infection
stud
presents
risk
rapidly
widely
distributing
within
or
between
countries.
Daily
blood
collection
four
after
intramuscular
inoculation
strain
‘Estonia
2014’
resulted
detection
genomes
early
2
dpi,
at
1
dpi
while
quality
remained
largely
unaffected.
Ultimately,
extended
semen,
7
14
were
positive
by
days
post
insemination,
all
35
insemination.
Twelve
out
13
pregnant
aborted
resorbed
onset
fever.
A
proportion
fetuses
originating
remaining
gilt
showed
both
abnormalities
replication
fetal
tissues.
present
evidence
efficient
via
AI
also
implanted
embryos.
These
results
underline
critical
role
could
play
transmission.
Язык: Английский
Pathology of African Swine Fever in Reproductive Organs of Mature Breeding Boars
Viruses,
Год журнала:
2023,
Номер
15(3), С. 729 - 729
Опубликована: Март 11, 2023
African
swine
fever
(ASF)
is
a
severe,
globally
important
disease
in
domestic
and
wild
pigs.
The
testing
of
alternative
transmission
routes
has
proven
that
the
ASF
virus
(ASFV)
can
be
efficiently
transmitted
to
sows
via
semen
from
infected
boars
through
artificial
insemination.
Boars
intramuscularly
inoculated
with
ASFV
strain
“Estonia
2014”
showed
grossly
microscopically
visible
changes
testis,
epididymis,
prostate,
vesicular
gland.
gross
lesions
included
hemorrhages
on
scrotum,
testicular
membranes,
parenchyma;
edema;
hydroceles;
proliferations
tunica
vaginalis.
Histopathologically,
vasculitis
perivasculitis
was
detected
testis
epididymis.
Subacutely
animals
further
revealed
degeneration
epididymal
tubules,
pointing
destruction
blood–testis
blood–epididymis
barriers
upon
progression.
This
confirmed
by
evidence
round
cells
sperm
abnormalities
at
later
time
points
after
infection.
histopathology
associated
presence
viral
DNA
infectious
virus,
limited
amount
antigens.
In
most
scenarios,
impact
these
reproductive
performance
long-term
persistence
probably
negligible
due
culling
animals.
However,
under
backyard
conditions
boar
populations,
males
will
remain
population
fate
should
evaluated.
Язык: Английский
The Male Reproductive Organs in African Swine Fever–Implications for Transmission
Опубликована: Ноя. 15, 2021
African
swine
fever
(ASF)
has
evolved
from
an
exotic
animal
disease
to
a
threat
global
pig
production.
An
important
avenue
for
wide-spread
transmission
of
diseases
is
the
dissemination
viruses
through
boar
semen
used
artificial
insemination
(AI).
In
this
context,
we
investigated
role
male
reproductive
organs
in
ASF.
Mature
domestic
boars
and
adolescent
wild
inoculated
with
different
ASF
virus
strains
were
by
means
virological
pathological
methods.
Additionally,
electron
microscopy
was
employed
investigate
vitro
sperm.
Viral
genome,
antigen
infectious
could
be
found
all
gonadal
tissues
accessory
sex
glands.
The
viral
mRNAs
mainly
mononuclear
cells
respective
tissues.
However,
some
other
cell
types,
including
Leydig,
endothelial
stromal
also
positive.
Using
RNAScope,
p72
mRNA
scattered
halo
epididymal
duct
epithelium
which
point
disruption
barrier.
No
direct
infection
spermatozoa
observed
immunohistochemistry
or
microscopy.
Taken
together,
our
results
strengthen
assumption
that
ASFV
can
transmitted
via
semen.
Future
studies
are
needed
explore
excretion
dynamics
efficiency.
Язык: Английский
Impact of ASFV Detergent Inactivation on Biomarkers in Serum and Saliva Samples
Pathogens,
Год журнала:
2022,
Номер
11(7), С. 750 - 750
Опубликована: Июнь 30, 2022
African
swine
fever
(ASF)
is
a
notifiable
viral
disease
of
domestic
and
wild
suids.
Despite
intensive
research
efforts,
the
pathogenesis
still
far
from
being
understood.
Analysis
biomarkers
in
different
body
fluids
may
supplement
traditional
studies.
As
reliable
protocols
are
often
established
laboratories
with
lower
biosafety,
inactivation
samples
crucial.
The
objective
this
study
was
to
find
procedure
that
inactivates
virus
while
preserving
for
downstream
analyses.
To
means,
three
were
employed,
namely
Tergitol-type
NP-40
(NP-40),
polyoxyethylene-p-t-octylphenol
(Triton
X-100)
one
95
°C
heating.
It
could
be
demonstrated
all
treated
0.5%
(v/v)
concentration
both
detergents
showed
an
absence
infectivity.
same
true
heated
samples.
However,
serum
not
suitable
Next,
impact
treatment
on
biomarker
readouts
assessed.
While
had
detection
biomarkers,
correlation
retained.
In
particular,
desired
detergent
more
accurate
measurements
achieving
efficient
inactivation.
Based
these
studies,
can
reliably
inactivated
most
analyses,
thus
broader
interdisciplinary
cooperation
possible.
Язык: Английский