European Spine Journal, Год журнала: 2024, Номер unknown
Опубликована: Сен. 20, 2024
Язык: Английский
Biosensors and Bioelectronics X, Год журнала: 2024, Номер 19, С. 100489 - 100489
Опубликована: Май 22, 2024
This article has tried to provide an overview of the most sophisticated microfluidic biosensors for identifying nucleic acids and proteins at site treatment. Microfluidics, which also automates sample preparation reduces processing time reagent consumption, enables analysis small quantities. Microfluidics biosensor technologies collaborate diagnostics point care with high throughput analysis, portability, disposability. The sensitivity selectivity requirements, false response errors, integration other essential modules are some challenges posed by this merger. broad categories protein-based DNA-based technology covered in review. Also, recent advancements coupling microfluidics, main potential solutions deploying point-of-care diagnostics, developments these areas have been discussed.
Язык: Английский
Процитировано
20
Materials Today Bio, Год журнала: 2025, Номер 31, С. 101613 - 101613
Опубликована: Фев. 25, 2025
Язык: Английский
Процитировано
3
Journal of Medicinal Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Янв. 4, 2025
The tedious synthesis and limited throughput biological evaluation remain a great challenge for discovering new proteolysis targeting chimera (PROTAC). To rapidly identify potential PROTAC lead compounds, we report platform named Auto-RapTAC. Based on the modular characteristic of molecule, streamlined workflow that integrates lab automation with "click chemistry" joint building-block libraries was constructed. This facilitates autonomous generation variety PROTACs, each distinct linkers E3 ligase ligands, all stored in biocompatible solutions. ready-for-screening (R4S) approach, when paired fluorescence-based assays, enables efficient assessment degradation activity high-throughput manner. further test capability platform, six PROTACs target CDK2, CDK12, BCL6 within mere 8-day time frame target. In all, this could find broad application not only but also rapid development novel heterobifunctional modalities.
Язык: Английский
Процитировано
2
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 874 - 874
Опубликована: Янв. 10, 2024
Metastatic progression is a complex, multistep process and the leading cause of cancer mortality. There growing evidence that emphasises significance epigenetic modification, specifically DNA methylation histone modifications, in influencing colorectal (CRC) metastasis. Epigenetic modifications influence expression genes involved various cellular processes, including pathways associated with These could contribute to metastatic by enhancing oncogenes silencing tumour suppressor genes. Moreover, specific alterations enable cells acquire invasive characteristics altering cell adhesion, migration, invasion-related pathways. Exploring involvement modification crucial for identifying biomarkers impact prediction metastasis CRC. This review provides summary potential CRC, particularly examines these biomarkers.
Язык: Английский
Процитировано
9
Expert Review of Proteomics, Год журнала: 2024, Номер 21(4), С. 149 - 168
Опубликована: Апрель 2, 2024
Introduction Saliva has gained increasing attention in the quest for disease biomarkers. Because it is a biological fluid that can be collected an easy, painless, and safe way, been increasingly studied identification of oral cancer This particularly important because often diagnosed at late stages with poor prognosis.
Язык: Английский
Процитировано
8
Tissue and Cell, Год журнала: 2025, Номер 95, С. 102850 - 102850
Опубликована: Март 11, 2025
Язык: Английский
Процитировано
1
Revista do Instituto de Medicina Tropical de São Paulo, Год журнала: 2023, Номер 65
Опубликована: Янв. 1, 2023
Toxoplasmosis is an infection of vast worldwide distribution whose etiologic agent Toxoplasma gondii. This disease can cause problems ranging from mild symptoms to serious conditions, such as encephalitis, miscarriage and blindness. Therefore, it utmost importance perform a diagnosis with reproducible techniques in order obtain good prognosis. The aim this review was analyze the efficiency toxoplasmosis diagnostic based on sensitivity specificity results. Five research platforms English language were used (Eric, Elsevier, Google Scholar, PubMed SciELO), which contained data toxoplasmosis. search selection performed for studies published prior June 2021. resulted inclusion 13 articles 2005 2020. revealed use different samples standardization serum, total blood, colostrum amniotic fluid. flow cytometry, lateral immunoassay qPCR showed 100% sensitivity, whereas ELISA, western blotting, RE-LAMP achieved specificity. Significantly, LAMP more accurate when likelihood ratio assessed. meta-analysis identified that ISAGA blotting have low values LIASON, ELFA using silica bioconjugate, also values. It noted wide range methods high choice method will be conditions its financial viability.
Язык: Английский
Процитировано
15
Translational Oncology, Год журнала: 2023, Номер 36, С. 101749 - 101749
Опубликована: Авг. 4, 2023
Mammalian apurinic/apyrimidinic endonuclease 1 (APE1, APEX1) is a multifunctional enzyme that maintains cellular homeostasis. It involved in the base excision repair (BER) pathway and plays key role radiation-induced DNA damage response. However, relationship between APE1-driven radiation resistance pyroptosis lung adenocarcinoma (LUAD) cells underlying molecular mechanisms remain unclear. We found APE1 was significantly upregulated LUAD tissues compared to para-carcinoma promoted proliferation invasion of vitro vivo. Mechanistically, inhibited by inactivating interferon gene stimulator (STING) via direct interaction with AIM2 DDX41, as detected RNA-seq co-immunoprecipitation. protects against induces radio-resistance targeting STING pathway. can induce negatively regulated interactions DDX41. Therefore, inhibitors should be considered enhance radiosensitivity improve patient prognosis therapeutic outcomes. Thus, play tumor immune microenvironment immunotherapy.
Язык: Английский
Процитировано
14
Therapeutic Advances in Medical Oncology, Год журнала: 2023, Номер 15
Опубликована: Янв. 1, 2023
Over the past decade, detection and analysis of liquid biopsy biomarkers such as circulating tumor cells (CTCs) DNA (ctDNA) have advanced significantly. They received recognition for their clinical usefulness in detecting cancer at an early stage, monitoring disease, evaluating treatment response. The emergence has been a helpful development, it offers minimally invasive, rapid, real-time monitoring, possible alternative to traditional tissue biopsies. In resource-limited settings, ideal platform should not only extract more CTCs or ctDNA from minimal sample volume but also accurately represent molecular heterogeneity patient's disease. This review covers novel strategies advancements CTC ctDNA-based platforms, including microfluidic applications comprehensive complexity. We discuss these systems' operational principles performance efficiencies, well future opportunities challenges implementation settings. addition, we emphasize importance integrated platforms that incorporate machine learning artificial intelligence accurate systems, which can greatly improve management enable precision diagnostics.
Язык: Английский
Процитировано
14
Entomologia Experimentalis et Applicata, Год журнала: 2024, Номер 172(5), С. 372 - 382
Опубликована: Фев. 14, 2024
Abstract Sericulture, the practice of rearing silkworms for production silk, is an essential agro‐based industry in several countries. However, are susceptible to a variety diseases caused by viruses, bacteria and parasites, which may have significant negative impact on global silk production. Traditional methods pathogen identification, such as microscopy laboratory culturing, limitations terms accuracy efficiency. The development molecular techniques identification has revolutionised field sericulture over last decade. Genomic DNA RNA‐based allow rapid accurate detection disease‐causing pathogens silkworms. Molecular diagnosis advantages traditional methods, including increased sensitivity specificity, shorter turnaround time ability detect that difficult culture or visualise under microscope. been applied important silkworms, Nosema sp., nucleopolyhedrovirus, cypovirus, iflavirus bidensovirus. use diagnostics immensely demand high‐quality increases globally assessment emerging associated with crop loss essential. Major advancements improvement application diagnosing widespread silkworm discussed.
Язык: Английский
Процитировано
4