International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(13), P. 11088 - 11088
Published: July 4, 2023
Danshen
has
been
widely
used
for
the
treatment
of
central
nervous
system
diseases.
We
investigated
effect
dihydroisotanshinone
I
(DT),
a
compound
extracted
from
Danshen,
as
well
corresponding
mechanisms
in
an
vitro-based
6-OHDA-induced
Parkinson's
disease
(PD)
model.
SH-SY5Y
human
neuroblastoma
cell
lines
were
pretreated
with
6-hydroxydopamine
(6-OHDA)
and
challenged
DT.
Subsequently,
viability
levels
reactive
oxygen
species
(ROS)
caspase-3
analyzed.
The
DT
on
6-OHDA-treated
cells
expression
core
circadian
clock
genes
measured
using
real-time
quantitative
polymerase
chain
reaction.
Our
results
indicated
that
attenuated
death
suppressed
ROS
caspase-3.
Moreover,
reversed
both
RNA
protein
BMAL1
SIRT1
cells.
Additionally,
inhibitor
reduced
viability.
activators
activated
BMAL1,
then
damaged
by
6-OHDA.
silencing
was
enhanced
resulted
downregulation
reduction
In
conclusion,
our
investigation
suggested
reduces
apoptosis,
including
antioxidative
due
to
ROS,
regulates
enhancing
suppressing
BMAL1.
may
possess
novel
therapeutic
potential
PD
future,
but
further
vivo
studies
are
still
needed.
Biosensors and Bioelectronics X,
Journal Year:
2024,
Volume and Issue:
19, P. 100489 - 100489
Published: May 22, 2024
This
article
has
tried
to
provide
an
overview
of
the
most
sophisticated
microfluidic
biosensors
for
identifying
nucleic
acids
and
proteins
at
site
treatment.
Microfluidics,
which
also
automates
sample
preparation
reduces
processing
time
reagent
consumption,
enables
analysis
small
quantities.
Microfluidics
biosensor
technologies
collaborate
diagnostics
point
care
with
high
throughput
analysis,
portability,
disposability.
The
sensitivity
selectivity
requirements,
false
response
errors,
integration
other
essential
modules
are
some
challenges
posed
by
this
merger.
broad
categories
protein-based
DNA-based
technology
covered
in
review.
Also,
recent
advancements
coupling
microfluidics,
main
potential
solutions
deploying
point-of-care
diagnostics,
developments
these
areas
have
been
discussed.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(2), P. 874 - 874
Published: Jan. 10, 2024
Metastatic
progression
is
a
complex,
multistep
process
and
the
leading
cause
of
cancer
mortality.
There
growing
evidence
that
emphasises
significance
epigenetic
modification,
specifically
DNA
methylation
histone
modifications,
in
influencing
colorectal
(CRC)
metastasis.
Epigenetic
modifications
influence
expression
genes
involved
various
cellular
processes,
including
pathways
associated
with
These
could
contribute
to
metastatic
by
enhancing
oncogenes
silencing
tumour
suppressor
genes.
Moreover,
specific
alterations
enable
cells
acquire
invasive
characteristics
altering
cell
adhesion,
migration,
invasion-related
pathways.
Exploring
involvement
modification
crucial
for
identifying
biomarkers
impact
prediction
metastasis
CRC.
This
review
provides
summary
potential
CRC,
particularly
examines
these
biomarkers.
Journal of Medicinal Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 4, 2025
The
tedious
synthesis
and
limited
throughput
biological
evaluation
remain
a
great
challenge
for
discovering
new
proteolysis
targeting
chimera
(PROTAC).
To
rapidly
identify
potential
PROTAC
lead
compounds,
we
report
platform
named
Auto-RapTAC.
Based
on
the
modular
characteristic
of
molecule,
streamlined
workflow
that
integrates
lab
automation
with
"click
chemistry"
joint
building-block
libraries
was
constructed.
This
facilitates
autonomous
generation
variety
PROTACs,
each
distinct
linkers
E3
ligase
ligands,
all
stored
in
biocompatible
solutions.
ready-for-screening
(R4S)
approach,
when
paired
fluorescence-based
assays,
enables
efficient
assessment
degradation
activity
high-throughput
manner.
further
test
capability
platform,
six
PROTACs
target
CDK2,
CDK12,
BCL6
within
mere
8-day
time
frame
target.
In
all,
this
could
find
broad
application
not
only
but
also
rapid
development
novel
heterobifunctional
modalities.
Expert Review of Proteomics,
Journal Year:
2024,
Volume and Issue:
21(4), P. 149 - 168
Published: April 2, 2024
Introduction
Saliva
has
gained
increasing
attention
in
the
quest
for
disease
biomarkers.
Because
it
is
a
biological
fluid
that
can
be
collected
an
easy,
painless,
and
safe
way,
been
increasingly
studied
identification
of
oral
cancer
This
particularly
important
because
often
diagnosed
at
late
stages
with
poor
prognosis.
Revista do Instituto de Medicina Tropical de São Paulo,
Journal Year:
2023,
Volume and Issue:
65
Published: Jan. 1, 2023
Toxoplasmosis
is
an
infection
of
vast
worldwide
distribution
whose
etiologic
agent
Toxoplasma
gondii.
This
disease
can
cause
problems
ranging
from
mild
symptoms
to
serious
conditions,
such
as
encephalitis,
miscarriage
and
blindness.
Therefore,
it
utmost
importance
perform
a
diagnosis
with
reproducible
techniques
in
order
obtain
good
prognosis.
The
aim
this
review
was
analyze
the
efficiency
toxoplasmosis
diagnostic
based
on
sensitivity
specificity
results.
Five
research
platforms
English
language
were
used
(Eric,
Elsevier,
Google
Scholar,
PubMed
SciELO),
which
contained
data
toxoplasmosis.
search
selection
performed
for
studies
published
prior
June
2021.
resulted
inclusion
13
articles
2005
2020.
revealed
use
different
samples
standardization
serum,
total
blood,
colostrum
amniotic
fluid.
flow
cytometry,
lateral
immunoassay
qPCR
showed
100%
sensitivity,
whereas
ELISA,
western
blotting,
RE-LAMP
achieved
specificity.
Significantly,
LAMP
more
accurate
when
likelihood
ratio
assessed.
meta-analysis
identified
that
ISAGA
blotting
have
low
values
LIASON,
ELFA
using
silica
bioconjugate,
also
values.
It
noted
wide
range
methods
high
choice
method
will
be
conditions
its
financial
viability.
Translational Oncology,
Journal Year:
2023,
Volume and Issue:
36, P. 101749 - 101749
Published: Aug. 4, 2023
Mammalian
apurinic/apyrimidinic
endonuclease
1
(APE1,
APEX1)
is
a
multifunctional
enzyme
that
maintains
cellular
homeostasis.
It
involved
in
the
base
excision
repair
(BER)
pathway
and
plays
key
role
radiation-induced
DNA
damage
response.
However,
relationship
between
APE1-driven
radiation
resistance
pyroptosis
lung
adenocarcinoma
(LUAD)
cells
underlying
molecular
mechanisms
remain
unclear.
We
found
APE1
was
significantly
upregulated
LUAD
tissues
compared
to
para-carcinoma
promoted
proliferation
invasion
of
vitro
vivo.
Mechanistically,
inhibited
by
inactivating
interferon
gene
stimulator
(STING)
via
direct
interaction
with
AIM2
DDX41,
as
detected
RNA-seq
co-immunoprecipitation.
protects
against
induces
radio-resistance
targeting
STING
pathway.
can
induce
negatively
regulated
interactions
DDX41.
Therefore,
inhibitors
should
be
considered
enhance
radiosensitivity
improve
patient
prognosis
therapeutic
outcomes.
Thus,
play
tumor
immune
microenvironment
immunotherapy.
Therapeutic Advances in Medical Oncology,
Journal Year:
2023,
Volume and Issue:
15
Published: Jan. 1, 2023
Over
the
past
decade,
detection
and
analysis
of
liquid
biopsy
biomarkers
such
as
circulating
tumor
cells
(CTCs)
DNA
(ctDNA)
have
advanced
significantly.
They
received
recognition
for
their
clinical
usefulness
in
detecting
cancer
at
an
early
stage,
monitoring
disease,
evaluating
treatment
response.
The
emergence
has
been
a
helpful
development,
it
offers
minimally
invasive,
rapid,
real-time
monitoring,
possible
alternative
to
traditional
tissue
biopsies.
In
resource-limited
settings,
ideal
platform
should
not
only
extract
more
CTCs
or
ctDNA
from
minimal
sample
volume
but
also
accurately
represent
molecular
heterogeneity
patient's
disease.
This
review
covers
novel
strategies
advancements
CTC
ctDNA-based
platforms,
including
microfluidic
applications
comprehensive
complexity.
We
discuss
these
systems'
operational
principles
performance
efficiencies,
well
future
opportunities
challenges
implementation
settings.
addition,
we
emphasize
importance
integrated
platforms
that
incorporate
machine
learning
artificial
intelligence
accurate
systems,
which
can
greatly
improve
management
enable
precision
diagnostics.
Entomologia Experimentalis et Applicata,
Journal Year:
2024,
Volume and Issue:
172(5), P. 372 - 382
Published: Feb. 14, 2024
Abstract
Sericulture,
the
practice
of
rearing
silkworms
for
production
silk,
is
an
essential
agro‐based
industry
in
several
countries.
However,
are
susceptible
to
a
variety
diseases
caused
by
viruses,
bacteria
and
parasites,
which
may
have
significant
negative
impact
on
global
silk
production.
Traditional
methods
pathogen
identification,
such
as
microscopy
laboratory
culturing,
limitations
terms
accuracy
efficiency.
The
development
molecular
techniques
identification
has
revolutionised
field
sericulture
over
last
decade.
Genomic
DNA
RNA‐based
allow
rapid
accurate
detection
disease‐causing
pathogens
silkworms.
Molecular
diagnosis
advantages
traditional
methods,
including
increased
sensitivity
specificity,
shorter
turnaround
time
ability
detect
that
difficult
culture
or
visualise
under
microscope.
been
applied
important
silkworms,
Nosema
sp.,
nucleopolyhedrovirus,
cypovirus,
iflavirus
bidensovirus.
use
diagnostics
immensely
demand
high‐quality
increases
globally
assessment
emerging
associated
with
crop
loss
essential.
Major
advancements
improvement
application
diagnosing
widespread
silkworm
discussed.
