Catalase-Knockout Complements the Radio-Sensitization Effect of Titanium Peroxide Nanoparticles on Pancreatic Cancer Cells
Molecules,
Год журнала:
2025,
Номер
30(3), С. 629 - 629
Опубликована: Янв. 31, 2025
In
previous
studies,
titanium
peroxide
nanoparticles
(PAA-TiOx
NPs)
with
surfaces
functionalized
using
polyacrylic
acid
(PAA)
and
hydrogen
(H2O2)
demonstrated
a
synergistic
effect
when
combined
X-ray
irradiation.
The
combination
generated
H2O2
reactive
oxygen
species
(ROS)
that
enhanced
the
irradiation
efficacy.
present
study,
we
examined
relationship
between
catalase
PAA-TiOx
NPs
sensitization
to
radiation
because
is
primary
antioxidant
enzyme
converts
water
oxygen.
Catalase-knockout
PANC-1
(dCAT)
cells
were
CRISPR/Cas9
system,
which
was
confirmed
by
suppression
of
expression
in
mRNA
protein
levels
resulted
an
81.7%
decrease
activity
compared
wild-type
cells.
Catalase
deficiency
found
increase
production
ROS,
particularly
hypoxia.
Also,
5
Gy
7-fold
survival
fraction
(SF;
p
<
0.01)
dCAT
rates
documented
Interestingly,
treatment
3
SF
similar
observed
treated
same
but
at
higher
dose
(5
Gy).
These
results
suggest
strategy
inhibition
could
be
used
establish
advanced
for
pancreatic
cancer
Язык: Английский
Cyanidin-3-O-glucoside inhibits the malignant progression of colorectal cancer by regulating Kruppel-like factor 4-mediated ERK/p38 signaling pathway
Toxicology and Applied Pharmacology,
Год журнала:
2025,
Номер
497, С. 117268 - 117268
Опубликована: Фев. 17, 2025
Язык: Английский
Acute and subacute toxicity evaluation of ZhenzhuXiaoji decoction in preclinical models: implications for safe clinical use
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 15, 2025
Background
ZhenzhuXiaoji
Decoction
(ZZXJD)
is
a
traditional
Chinese
medicine
formulation
composed
of
five
herbs:
Ligustrum
lucidum
,
Curcuma
zedoaria
Prunella
vulgaris
Hedyotis
diffusa
and
Glycyrrhiza
uralensis
developed
for
the
treatment
hepatocellular
carcinoma
(HCC).
Although
early
studies
have
demonstrated
therapeutic
potential
ZZXJD,
its
safety
profile,
particularly
regarding
toxicity,
remains
underexplored.
This
study
aims
to
evaluate
both
pharmacological
effects
toxicity
ZZXJD
in
preclinical
models
determine
clinical
applicability.
Study
design
Methods
employed
vitro
vivo
experiments
assess
ZZXJD.
HHL-5
HEK-293
cell
lines
were
treated
with
at
varying
concentrations
(125,
250,
500,
1,000
μg/mL)
24,
48,
72
h
on
viability,
apoptosis,
necrosis.
Acute
subacute
conducted
male
female
mice,
including
assessments
behavioral
changes,
body
weight,
organ
liver/kidney
functions.
Additionally,
routine
blood
tests
performed
identify
immunostimulatory
effects.
Results
In
that
inhibited
proliferation
cells
dose-dependent
manner
induced
apoptosis
studies,
mice
low
mid-dose
groups
exhibited
no
significant
whereas
high-dose
group
showed
transient
alterations
liver
kidney
function
markers,
mice.
These
changes
reversible
following
cessation.
Blood
indicated
increased
lymphocyte
monocyte
counts
mice;
however,
these
increases
not
statistically
significant.
Organ
weight
histopathological
analyses
revealed
signs
doses.
Conclusion
Treatment
standard
dosage
did
produce
acute
or
toxic
functions
suggesting
continued
use
cancer
treatment.
However,
abnormalities
markers
observed
higher
Thus,
regular
monitoring
recommended
during
use,
especially
when
doses
are
employed.
Язык: Английский
Aronia-derived anthocyanins and metabolites ameliorate TNFα-induced disruption of myogenic differentiation in satellite cells
Biochemical and Biophysical Research Communications,
Год журнала:
2024,
Номер
733, С. 150687 - 150687
Опубликована: Сен. 14, 2024
Язык: Английский
Camote de pulpa morada (Ipomoea batatas L.) y sus antocianinas: de postre tradicional a fuente de colorante natural
Revista Agraria,
Год журнала:
2024,
Номер
22(1), С. 10 - 14
Опубликована: Март 13, 2024
Sweet
potato
is
a
food
of
great
relevance
worldwide;
however,
its
consumption
in
Mexico
remains
low
despite
nutritional
value
and
the
diversity
available
varieties.
Consumer
demand
for
more
natural
less
processed
recent
years
foods
has
increased.
A
new,
opportunity
emerged
industry
to
replace
artificial
colorants
with
alternatives.In
this
context,
obtaining
pigments
from
purple-fleshed
sweet
potatoes
presents
viable
option
due
their
high
anthocyanin
content.
These
are
ideal
candidates
replacing
synthetic
colorants,
as
they
can
produce
variety
shades,
ranging
reds
blues,
depending
on
extraction
conditions.
This
versatility
broadens
application
different
products.
In
addition
being
an
using
color
industry,
represent
promote
leverage
potential
health
benefits.
Highlighting
aims
increase
visibility,
encourage
use,
take
advantages
Язык: Английский