Integrated Network Pharmacology, Machine Learning and Experimental Validation to Identify the Key Targets and Compounds of TiaoShenGongJian for the Treatment of Breast Cancer

OncoTargets and Therapy, Год журнала: 2025, Номер Volume 18, С. 49 - 71

Опубликована: Янв. 1, 2025

TiaoShenGongJian (TSGJ) decoction, a traditional Chinese medicine for breast cancer, has unknown active compounds, targets, and mechanisms. This study identifies TSGJ's key targets compounds cancer treatment through network pharmacology, machine learning, experimental validation. Bioactive components of TSGJ were identified from the TCMSP database, cancer-related GeneCards, PharmGkb, RNA-seq datasets. Intersection these revealed therapeutic TSGJ. PPI analysis was performed via STRING, learning methods (SVM, RF, GLM, XGBoost) validated by GSE70905, GSE70947, GSE22820, TCGA-BRCA Pathway analyses molecular docking performed. core compounds' effectiveness confirmed MTT RT-qPCR assays. 160 common identified, with 30 hub analysis. Five predictive (HIF1A, CASP8, FOS, EGFR, PPARG) screened SVM. Their diagnostic, biomarker, immune, clinical values validated. Quercetin, luteolin, baicalein as components. Molecular their strong affinities predicted targets. These modulated induced cytotoxicity in cell lines similar way reveals main against supporting its potential prevention treatment.

Язык: Английский

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Unraveling the Mechanisms of Shaoyang Shenggu Decoction in Treating Knee Osteoarthritis through Mass Spectrometry and Bioinformatics

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119835 - 119835

Опубликована: Апрель 1, 2025

Язык: Английский

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Deciphering the Role of LncRNAs in Osteoarthritis: Inflammatory Pathways Unveiled

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 6563 - 6581

Опубликована: Сен. 1, 2024

Long non-coding RNA (LncRNA), with transcripts over 200 nucleotides in length, play critical roles numerous biological functions and have emerged as significant players the pathogenesis of osteoarthritis (OA), an inflammatory condition traditionally viewed a degenerative joint disease. This review comprehensively examines influence LncRNA on processes driving OA progression, focusing their role regulating gene expression, cellular activities, pathways. Notably, LncRNAs such MALAT1, H19, HOTAIR are upregulated exacerbate milieu by modulating key signaling pathways like NF-κB, TGF-β/SMAD, Wnt/β-catenin. Conversely, MEG3 GAS5, which downregulated OA, show potential dampening responses protecting against cartilage degradation influencing miRNA interactions cytokine production. By enhancing our understanding LncRNA' inflammation, we can better leverage them biomarkers for disease develop innovative therapeutic strategies management. paper aims to delineate mechanisms propose novel targets intervention.

Язык: Английский

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Yiqi Yangxue formula inhibits cartilage degeneration in knee osteoarthritis by regulating LncRNA-UFC1/miR-34a/MMP-13 axis

Journal of Ethnopharmacology, Год журнала: 2024, Номер 337, С. 118930 - 118930

Опубликована: Окт. 10, 2024

Язык: Английский

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