Macromolecular Bioscience,
Год журнала:
2024,
Номер
24(6)
Опубликована: Янв. 24, 2024
Hydrogels
are
3D
networks
swollen
with
water.
They
biocompatible,
strong,
and
moldable
emerging
as
a
promising
biomedical
material
for
regenerative
medicine
tissue
engineering
to
deliver
therapeutic
genes.
The
excellent
natural
extracellular
matrix
simulation
properties
of
hydrogels
enable
them
be
co-cultured
cells
or
enhance
the
expression
viral
non-viral
vectors.
Its
biocompatibility,
high
strength,
degradation
performance
also
make
action
process
carriers
in
tissues
more
ideal,
making
it
an
ideal
material.
It
has
been
shown
that
hydrogel-based
gene
delivery
technologies
have
potential
play
therapy-relevant
roles
organs
such
bone,
cartilage,
nerve,
skin,
reproductive
organs,
liver
animal
experiments
preclinical
trials.
This
paper
reviews
recent
articles
on
explains
manufacture,
applications,
developmental
timeline,
limitations,
future
directions
techniques.
Abstract
In
recent
decades,
research
on
Extracellular
Vesicles
(EVs)
has
gained
prominence
in
the
life
sciences
due
to
their
critical
roles
both
health
and
disease
states,
offering
promising
applications
diagnosis,
drug
delivery,
therapy.
However,
inherent
heterogeneity
complex
origins
pose
significant
challenges
preparation,
analysis,
subsequent
clinical
application.
This
review
is
structured
provide
an
overview
of
biogenesis,
composition,
various
sources
EVs,
thereby
laying
groundwork
for
a
detailed
discussion
contemporary
techniques
preparation
analysis.
Particular
focus
given
state‐of‐the‐art
technologies
that
employ
microfluidic
non‐microfluidic
platforms
EV
processing.
Furthermore,
this
discourse
extends
into
innovative
approaches
incorporate
artificial
intelligence
cutting‐edge
electrochemical
sensors,
with
particular
emphasis
single
proposes
current
outlines
prospective
avenues
future
research.
The
objective
motivate
researchers
innovate
expand
methods
analysis
fully
unlocking
biomedical
potential.
iScience,
Год журнала:
2024,
Номер
27(4), С. 109350 - 109350
Опубликована: Фев. 27, 2024
Colorectal
cancer
(CRC)
ranks
as
the
second
leading
cause
of
cancer-related
mortality,
with
metastasis
being
primary
determinant
poor
prognosis
in
patients.
Investigating
molecular
mechanisms
underlying
CRC
is
currently
a
prominent
and
challenging
area
research.
Exosomes,
crucial
intercellular
communication
mediators,
facilitate
transfer
metabolic
genetic
information
from
cells
origin
to
recipient
cells.
Their
roles
mediating
exchange
between
immune
cells,
fibroblasts,
other
cell
types
are
pivotal
reshaping
tumor
microenvironment,
regulating
key
biological
processes
such
invasion,
migration,
formation
pre-metastatic
niche.
This
article
comprehensively
examines
function
mechanism
exosomes
derived
different
metastasis,
while
also
presenting
an
outlook
on
current
research
advancements
future
application
prospects.
The
aim
offer
distinctive
perspective
that
contributes
accurate
diagnosis
rational
treatment
strategies
for
CRC.
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Фев. 15, 2024
Abstract
In
recent
decades,
emerging
data
have
highlighted
the
critical
role
of
extracellular
vesicles
(EVs),
especially
(exosomes)
Exos,
in
progression
and
development
several
cancer
types.
These
nano-sized
are
released
by
different
cell
lineages
within
niche
maintain
a
suitable
platform
for
interchange
various
signaling
molecules
paracrine
manner.
Based
on
studies,
Exos
can
transfer
oncogenic
factors
to
other
cells,
alter
activity
immune
tumor
microenvironment,
leading
expansion
cells
metastasis
remote
sites.
It
has
been
indicated
that
cell-to-cell
crosstalk
is
so
complicated
wide
array
involved
this
process.
How
which
mechanisms
regulate
behavior
non-cancer
at
center
debate.
Here,
we
scrutinize
molecular
parenchyma.
Besides,
tumoricidal
properties
from
stem
(SC)
types
discussed
detail.
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Апрель 8, 2024
Abstract
Background
Prostate
cancer
(PCa)
is
a
prevalent
malignancy
in
men
worldwide,
ranking
as
the
second
leading
cause
of
cancer-related
death
Western
countries.
Various
PCa
hormone
therapies,
such
androgen
receptor
(AR)-antagonists
or
supraphysiological
level
(SAL)
reduce
cell
proliferation.
However,
treated
cells
may
influence
growth
neighboring
through
secreted
exosomes
tumor
microenvironment
(TME).
Here,
change
protein
content
from
treatment
with
different
AR-antagonists
SAL
has
been
analyzed.
Methods
Isolation
via
ultracentrifugation
human
LNCaP
AR-agonist
and
various
AR-antagonists;
analysis
cellular
senescence
by
detection
associated
beta
galactosidase
activity
(SA
β-Gal);
blotting
immunofluorescence
staining;
Mass
spectrometry
(MS-spec)
bioinformatic
analyses
to
identify
ligand-specific
exosomal
proteins.
Growth
assays
analyze
on
non-treated
cells.
Results
MS-spec
identified
proteins
exosomes.
One
thousand
seventy
were
up-
52
downregulated
whereas
enzalutamide
upregulated
151
42
The
prediction
indicates
an
up-regulation
pro-proliferative
pathways.
AR
ligands
augment
hub
factors
that
include
AKT1,
CALM1,
PAK2
CTNND1.
Accordingly,
functional
confirmed
isolated
AR-ligand
promote
untreated
Conclusion
data
suggest
cargo
controlled
-antagonists
distinct
among
AR-antagonists.
Further,
might
TME.
This
finding
sheds
light
into
complex
interplay
between
signaling
exosome-mediated
communication
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(8), С. 4378 - 4378
Опубликована: Апрель 16, 2024
Mesenchymal
stem
cell-derived
exosomes
(MSC-Exos)
are
nano-sized
extracellular
vesicles
which
contain
various
MSC-sourced
anti-fibrotic,
immunoregulatory
and
angio-modulatory
proteins
(growth
factors,
cytokines,
chemokines),
lipids,
nucleic
acids
(messenger
RNA
microRNAs).
Due
to
their
lipid
envelope,
MSC-Exos
easily
by-pass
all
barriers
in
the
body
deliver
cargo
directly
target
cells,
modulating
viability,
proliferation,
phenotype
function.
The
results
obtained
recently
published
experimental
studies
demonstrated
beneficial
effects
of
treatment
lung
fibrosis.
reduced
activation
fibroblasts
prevented
differentiation
myofibroblasts.
By
delivering
factors
lung-infiltrated
monocytes
T
modulate
function,
alleviating
on-going
inflammation
may
also
serve
as
vehicles
for
delivery
anti-fibrotic
immunomodulatory
agents,
enabling
enhanced
attenuation
Although
numerous
pre-clinical
have
therapeutic
potential
pulmonary
fibrosis,
there
several
challenges
that
currently
hinder
clinical
implementation.
Therefore,
this
review
article,
we
summarized
current
knowledge
discussed
future
perspectives
regarding
molecular
cellular
mechanisms
were
responsible
anti-inflammatory
properties
MSC-Exos,
paving
way
use
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Май 14, 2024
Abstract
Due
to
vincristine
sulfate’s
(VCR
sulfate)
toxicity
and
non-specific
targeting,
which
might
adversely
damage
healthy
cells,
its
clinical
application
is
restricted.
In
this
study,
we
loaded
VCR
sulfate
on
exosomes
generated
from
mesenchymal
stem
cells
(MSCs)
enhance
targeted
distribution.
Exosomes
are
able
deliver
molecules
specific
tissues
have
therapeutic
potential.
isolated
MSCs,
using
probe-sonication
approach
them
with
sulfate.
Using
SRB
assay,
the
cytotoxicity
of
sulfate-Exo
was
assessed
in
T47D
breast
cancer
results
were
contrasted
those
free
Then
We
labeled
markers
(CD44+/CD24−)
cell
line
assess
targeting
effectiveness
flow
cytometry.
Our
showed
that
nearly
same
as
Flow
cytometry
analysis
revealed
VRC
more
effectively
MSCs
than
study
shows
loading
MSCs-derived
can
improve
their
delivery
lessen
side
effects.
Additional
research
required
determine
sulfate-Exo’s
vivo
safety
strategies.
Journal of Biological Engineering,
Год журнала:
2024,
Номер
18(1)
Опубликована: Июнь 6, 2024
Exosomes
are
nanovesicles
with
multiple
components
used
in
several
applications.
Mesenchymal
stem
cells
(MSCs)
well
known
for
their
great
potential
clinical
MSC-derived
exosomes
(MSC-Exos)
have
been
shown
to
mediate
tissue
regeneration
various
diseases,
including
neurological,
autoimmune,
and
inflammatory
cancer,
ischemic
heart
disease,
lung
injury,
liver
fibrosis.
They
can
modulate
the
immune
response
by
interacting
effector
presence
of
anti-inflammatory
compounds
involved
intercellular
communication
through
types
cargo.
This
review
summarizes
MSC-Exos-mediated
cardiovascular,
liver,
kidney,
articular
cartilage,
oral
In
addition,
we
discuss
challenges
prospects
MSC-Exos
regeneration.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Июнь 18, 2024
Abstract
Recently,
the
significant
benefits
of
cancer
immunotherapy
for
most
cancers
have
been
demonstrated
in
clinical
and
preclinical
studies.
However,
efficacy
these
immunotherapies
gliomas
is
limited,
owing
to
restricted
drug
delivery
insufficient
immune
activation.
As
carriers,
exosomes
offer
advantages
low
toxicity,
good
biocompatibility,
intrinsic
cell
targeting,
which
could
enhance
glioma
efficacy.
a
review
exosome-based
systems
has
not
presented.
This
introduces
current
problems
role
addressing
issues.
Meanwhile,
preparation
application
strategies
are
discussed,
especially
enhancing
immunogenicity
reversing
immunosuppressive
tumor
microenvironment.
Finally,
we
briefly
describe
challenges
translation.
We
anticipate
that
this
will
guide
use
as
carriers
immunotherapy.
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