Modern advances in spinal cord regeneration: hydrogel combined with neural stem cells

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июнь 27, 2024

Severe spinal cord injuries (SCI) lead to loss of functional activity the body below injury site, affect a person’s ability self-care and have direct impact on performance. Due structural features role in body, consequences SCI cannot be completely overcome at expense endogenous regenerative potential and, developing over time, severe complications years after injury. Thus, primary task this type treatment is create artificial conditions for growth damaged nerve fibers through area SCI. Solving problem possible using tissue neuroengineering involving technology replacing natural environment with synthetic matrices (for example, hydrogels) combination stem cells, particular, neural/progenitor cells (NSPCs). This approach can provide maximum stimulation support axons neurons their myelination. In review, we consider currently available options improving condition (use NSC transplantation or/and replacement matrix, specifically hydrogel). We emphasise expediency effectiveness hydrogel matrix + NSCs complex system used reconstruction Since such (a engineering cell therapy), our opinion, allows not only creation supporting regeneration or mechanical cord, but also strengthen regeneration, prevent spread inflammatory process, promote restoration lost reflex, motor sensory functions injured cord.

Язык: Английский

---

Conductive Hydrogels as an “Innovative Healer” for the treatment of diabetic wounds

Materials Chemistry Frontiers, Год журнала: 2024, Номер 8(18), С. 2944 - 2977

Опубликована: Янв. 1, 2024

Conductive hydrogels, with their electrical stimulation effects and properties, are potential candidates for treating diabetic wounds.

Язык: Английский

---

Multifunctional magneto-electric and exosome-loaded hydrogel enhances neuronal differentiation and immunoregulation through remote non-invasive electrical stimulation for neurological recovery after spinal cord injury

Bioactive Materials, Год журнала: 2025, Номер 48, С. 510 - 528

Опубликована: Фев. 28, 2025

Язык: Английский

---

DNA Hydrogels in Tissue Engineering: From Molecular Design to Next‐Generation Biomedical Applications

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Abstract DNA hydrogels have emerged as promising materials in tissue engineering due to their biocompatibility, programmability, and responsiveness stimuli. Synthesized through physical chemical crosslinking, these can be categorized into functionalized types, such those based on aptamers, stimuli‐responsive types that react pH, temperature, light. This review highlights applications engineering, including drug delivery, cell culture, biosensing, gene editing. encapsulate therapeutic agents, support growth, respond dynamically environmental changes, making them ideal for engineering. A comprehensive bibliometric analysis is included, identifying key research trends emerging areas of interest hydrogel design, synthesis, biomedical applications. The provides a deeper understanding the field's development future directions. Challenges mechanical strength, stability, biosafety persist, but integration AI design shows promise advancing functionality clinical

Язык: Английский

---

Advancements and Prospects of pH-Responsive Hydrogels in Biomedicine

Gels, Год журнала: 2025, Номер 11(4), С. 293 - 293

Опубликована: Апрель 15, 2025

As an intelligent polymer material, pH-sensitive hydrogels exhibit the capability to dynamically sense alterations in ambient pH levels and subsequently initiate corresponding physical or chemical responses, including swelling, contraction, degradation, ion exchange. Given significant variations inherent human pathophysiological microenvironments, particularly tumor tissues, inflammatory lesions, gastrointestinal system, these smart materials demonstrate remarkable application potential across diverse domains such as targeted drug delivery systems, regenerative medicine engineering, biosensing, disease diagnostics. Recent breakthroughs nanotechnology precision have substantially propelled advancements design of pH-responsive hydrogels. This review systematically elaborates on current research progress future challenges regarding biomedical applications, with particular emphasis their stimulus–response mechanisms, fabrication methodologies, multifunctional integration strategies, scenarios.

Язык: Английский

---

Bone marrow mesenchymal stem cells-derived exosomal miR-24-3p alleviates spinal cord injury by targeting MAPK9 to inhibit the JNK/c-Jun/c-Fos pathway

Archives of Biochemistry and Biophysics, Год журнала: 2025, Номер 769, С. 110434 - 110434

Опубликована: Апрель 22, 2025

Язык: Английский

---

Multidimensional exploration of hydrogels as biological scaffolds for spinal cord regeneration: mechanisms and future perspectives

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Апрель 23, 2025

Spinal cord injury (SCI) is a severe condition that frequently leads to permanent disabilities and neurological dysfunction. Its progression driven by multifaceted pathophysiology, encompassing direct trauma, secondary cascades, intricate cellular molecular responses. While current therapies focus on alleviating symptoms restoring functionality, achieving effective neural regeneration in the spinal continues be significant challenge. Hydrogels, recognized for their exceptional biocompatibility, conductivity, injectability, have shown great potential as advanced scaffolds support neuronal axonal regeneration. Recently, these materials attracted interest field of SCI rehabilitation research. This review concludes recent progress hydrogel-based strategies rehabilitation, emphasizing distinct properties, underlying mechanisms, integration with bioactive molecules, stem cells, complementary biomaterials. Hydrogels foster providing tailored microenvironment, while features such self-repair, electrical controlled drug release significantly enhance therapeutic experimental models. explores hydrogel technologies applications, underscoring address challenges treatment paving way future clinical implementation.

Язык: Английский

---

cxcl18b-defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina

Опубликована: Апрель 30, 2025

In lower vertebrates, retinal Müller glia (MG) exhibit a life-long capacity of cell-cycle re-entry to regenerate neurons following the injury. However, mechanism driving such injury-induced MG remains incompletely understood. Combining single-cell transcriptomic analysis and in-vivo clonal analysis, we identified previously undescribed cxcl18b -defined transitional states as essential routes towards proliferation green/red cone (G/R cone) ablation. Microglial inflammation was necessary for triggering these states, which expressed gene modules shared by cells ciliary marginal zone (CMZ) where adult neurogenesis takes place. Functional studies redox properties further demonstrated regulatory role nitric oxide (NO) produced Nos2b in proliferation. Finally, developed viral-based strategy specifically disrupt nos2b revealed effect state-specific NO signaling. Our findings elucidate redox-related underlying re-entry, providing insights into species-specific mechanisms vertebrate retina regeneration.

Язык: Английский

---

cxcl18b-defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina

Опубликована: Апрель 30, 2025

In lower vertebrates, retinal Müller glia (MG) exhibit a life-long capacity of cell-cycle re-entry to regenerate neurons following the injury. However, mechanism driving such injury-induced MG remains incompletely understood. Combining single-cell transcriptomic analysis and in-vivo clonal analysis, we identified previously undescribed cxcl18b -defined transitional states as essential routes towards proliferation green/red cone (G/R cone) ablation. Microglial inflammation was necessary for triggering these states, which expressed gene modules shared by cells ciliary marginal zone (CMZ) where adult neurogenesis takes place. Functional studies redox properties further demonstrated regulatory role nitric oxide (NO) produced Nos2b in proliferation. Finally, developed viral-based strategy specifically disrupt nos2b revealed effect state-specific NO signaling. Our findings elucidate redox-related underlying re-entry, providing insights into species-specific mechanisms vertebrate retina regeneration.

Язык: Английский

---

Transcranial magnetic stimulation through attenuating blood-spinal cord barrier disruption and reducing inflammatory response to improve motor function in rats with spinal cord injury

Brain Research Bulletin, Год журнала: 2025, Номер 226, С. 111385 - 111385

Опубликована: Май 13, 2025

Previous therapies for spinal cord injury (SCI) typically focus on the lesion site, neglecting interconnected brain areas. Transcranial magnetic stimulation (TMS) is an emerging non-invasive neuromodulation technique, demonstrating potential in modulating primary motor cortex to enhance SCI recovery. The modified Allen's method was used establish rat model. High-frequency repetitive TMS (HF-rTMS) intervention initiated second day after modeling and continued 56 days. Bioinformatics analysis identified key genes involved pathological process, including MMP9, IL-1β, IL-18. This study explored functions mechanisms of these HF-rTMS-mediated recovery rats. Western blotting reveals that HF-rTMS decreases active-MMP9/pro-MMP9, TNF-α, IL-18 proteins' expression, while increases β-DG, Occludin, Claudin-5, ZO-1 expression injured (P < 0.001). Immunofluorescence staining further shows reduces MMP9 positive cells, enhances cells Evans Blue indicates blood-spinal barrier (BSCB) permeability following injury, ELISA results demonstrate attenuates serum levels pro-inflammatory cytokines. Motor-evoked potentials (MEP) assessment, HE staining, BBB score show shortens MEP latency, amplitude, damage improves function These findings reveal may be associated with suppressing activation, protecting tight junction proteins, diminishing basement membrane destruction, maintaining BSCB integrity. Simultaneously, it alleviate cytokine-induced inflammation, thereby reducing tissue promoting SCI.

Язык: Английский

---