Ganoderma lucidum polysaccharide attenuates retinal ischemia-reperfusion injury by regulating microglial M1/M2 polarization, suppressing neuroinflammation and inhibiting JAK2/STAT3 pathway
Biochemistry and Biophysics Reports,
Год журнала:
2025,
Номер
41, С. 101926 - 101926
Опубликована: Янв. 29, 2025
Язык: Английский
Tetrahedral Framework Nucleic Acid‐Based Delivery of DJ‐1‐saRNA Prevent Retinal Ischaemia–Reperfusion Injury via Inhibiting Ferroptosis
Cell Proliferation,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 20, 2025
ABSTRACT
Retinal
ischaemia/reperfusion
injury
(RI/RI)
is
the
primary
pathophysiological
mechanism
underlying
retinal
ischaemic
diseases,
potentially
resulting
in
significant
and
irreversible
visual
impairment.
Currently,
there
are
no
effective
treatments
available
for
RI/RI,
oxidative
stress
a
critical
factor
that
contributes
to
associated
damage.
DJ‐1,
an
important
endogenous
antioxidant,
has
been
proposed
as
promising
therapeutic
agent
RI/RI
owing
its
potential
overexpression.
In
this
study,
tetrahedral
frame
nucleic
acids
(tFNAs)
were
utilised
delivery
vehicle
DJ‐1
small
activating
RNA
(saRNA),
synthesis
of
novel
nanocomposite
(tFNAs‐DJ‐1‐saRNA).
vitro
experiments
demonstrated
tFNAs
effectively
delivered
DJ‐1‐saRNA
R28
cells,
thus
exerting
repair
effect
on
injury.
vivo
investigations
revealed
intravitreal
injection
tFNAs‐DJ‐1‐saRNA
facilitated
gene
expression
mitigated
atrophy
induced
by
RI/RI.
Mechanistically,
activated
xCT/GPX4
pathway,
thereby
inhibiting
ferroptosis,
reducing
ganglion
cell
damage
protecting
tissue.
conclusion,
study
complex
can
ameliorate
suggesting
treatment
diseases.
Язык: Английский
Frontier applications of retinal nanomedicine: progress, challenges and perspectives
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Фев. 25, 2025
The
human
retina
is
a
fragile
and
sophisticated
light-sensitive
tissue
in
the
central
nervous
system.
Unhealthy
retinas
can
cause
irreversible
visual
deterioration
permanent
vision
loss.
Effective
therapeutic
strategies
are
restricted
to
treatment
or
reversal
of
these
conditions.
In
recent
years,
nanoscience
nanotechnology
have
revolutionized
targeted
management
retinal
diseases.
Pharmaceuticals,
theranostics,
regenerative
medicine,
gene
therapy,
prostheses
indispensable
for
interventions
been
significantly
advanced
by
nanomedical
innovations.
Hence,
this
review
presents
novel
insights
into
use
versatile
nanomaterial-based
nanocomposites
frontier
applications,
including
non-invasive
drug
delivery,
theranostic
contrast
agents,
nanoagents,
stem
cell-based
optogenetics
prostheses,
which
mainly
reported
within
last
5
years.
Furthermore,
progress,
potential
challenges,
future
perspectives
field
highlighted
discussed
detail,
may
shed
light
on
clinical
translations
ultimately,
benefit
patients
with
disorders.
Язык: Английский
Glial connexins in glaucoma
Frontiers in Neuroscience,
Год журнала:
2025,
Номер
19
Опубликована: Апрель 9, 2025
Glial
cells
play
a
crucial
role
in
maintaining
central
nervous
system
(CNS)
homeostasis
and
facilitating
the
repair
of
neural
tissue
following
injury.
The
regulation
neuroglia
may
serve
as
safe
effective
strategy
for
modulating
neuroinflammatory
responses
restoring
glial
defense
functions.
Given
that
network
is
composed
connexin
(CX)
proteins,
its
neuroprotective
extensive.
Therefore,
connexins
should
be
considered
functional
“bridges”
within
this
network.
This
review
examines
evidence
active
involvement
networks
neuroinflammation
under
both
physiological
pathological
conditions
summarizes
CXs
glaucoma.
Finally,
potential
therapeutic
strategies
glaucoma
are
explored.
Язык: Английский
Lycium Barbarum Glycopeptide Alleviates Retinal Inflammation by Suppressing Microglial M1 Polarization via NF-κB/MAPK Pathways
Опубликована: Май 29, 2024
Microglia-related
retinal
inflammation
is
associated
with
a
variety
of
diseases.
Lycium
barbarum
Glycopeptide
(LBGP)
the
main
active
component
polysaccharide
(LBP)
isolated
from
barbarum,
which
possesses
antioxidation,
neuroprotection,
and
immunoregulation.
However,
whether
LBGP
has
beneficial
effects
on
LPS/IFN-γ
(L/I)
induced
microglial
activation
its
underlying
mechanism
remains
to
be
elucidated.
In
this
study,
we
observed
anti-inflammatory
properties
in
microglia
activated
by
LPS
IFN-γ
(L/I),
examining
both
primary
BV2
cells.
Cells
were
pretreated
for
2h
before
stimulated
24h.
The
dosages
cells
evaluated
using
CCK-8
assay.
morphological
changes
an
optical
microscope.
expression
cytokines
inflammatory
mediators
measured
Griess
assay,
ELISA,
qPCR,
immunofluorescence.
Western
blotting
was
used
assess
MAPK,
NF-κB
signal
transducer.
pre-treatment
significantly
reversed
L/I-induced
alterations
area,
perimeter,
Feret's
diameter,
roundness.
alleviated
switched
M1
pro-inflammatory
phenotype
M2
downregulating
markers
(iNOS,
IL-1β,
IL-6,
COX2,
CD16,
CD86)
upregulating
(arginase
1
CD206).
Furthermore,
attenuated
MAPK
pathways
L/I-activated
summary,
it
suggested
that
pretreatment
regulating
microglial(M1/M2)
polarization
via
signaling
pathways.
Язык: Английский